Biopreservation of living tissue engineered nerve grafts
Tissue engineered nerve grafts (TENGs) built from living neurons and aligned axon tracts offer a revolutionary new approach as “living scaffolds” to bridge major peripheral nerve defects. Clinical application, however, necessitates sufficient shelf-life to allow for shipping from manufacturing facil...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2021-08-01
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| Series: | Journal of Tissue Engineering |
| Online Access: | https://doi.org/10.1177/20417314211032488 |
| _version_ | 1819129294055538688 |
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| author | Robert B Shultz Kritika S Katiyar Franco A Laimo Justin C Burrell Kevin D Browne Zarina S Ali Daniel K Cullen |
| author_facet | Robert B Shultz Kritika S Katiyar Franco A Laimo Justin C Burrell Kevin D Browne Zarina S Ali Daniel K Cullen |
| author_sort | Robert B Shultz |
| collection | DOAJ |
| description | Tissue engineered nerve grafts (TENGs) built from living neurons and aligned axon tracts offer a revolutionary new approach as “living scaffolds” to bridge major peripheral nerve defects. Clinical application, however, necessitates sufficient shelf-life to allow for shipping from manufacturing facility to clinic as well as storage until use. Here, hypothermic storage in commercially available hibernation media is explored as a potential biopreservation strategy for TENGs. After up to 28 days of refrigeration at 4℃, TENGs maintain viability and structure in vitro . Following transplantation into 1 cm rat sciatic defects, biopreserved TENGs routinely survive and persist for at least 2 weeks and recapitulate pro-regenerative mechanisms of fresh TENGs, including the ability to recruit regenerating host tissue into the graft and extend neurites beyond the margins of the graft. The protocols and timelines established here serve as important foundational work for the manufacturing, storage, and translation of other neuron-based tissue engineered therapeutics. |
| first_indexed | 2024-12-22T08:41:25Z |
| format | Article |
| id | doaj.art-d8746a4133624040a6146f1113537dc1 |
| institution | Directory Open Access Journal |
| issn | 2041-7314 |
| language | English |
| last_indexed | 2024-12-22T08:41:25Z |
| publishDate | 2021-08-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Tissue Engineering |
| spelling | doaj.art-d8746a4133624040a6146f1113537dc12022-12-21T18:32:14ZengSAGE PublishingJournal of Tissue Engineering2041-73142021-08-011210.1177/20417314211032488Biopreservation of living tissue engineered nerve graftsRobert B Shultz0Kritika S Katiyar1Franco A Laimo2Justin C Burrell3Kevin D Browne4Zarina S Ali5Daniel K Cullen6Axonova Medical, LLC, Philadelphia, PA, USAAxonova Medical, LLC, Philadelphia, PA, USACenter for Brain Injury and Repair, Department of Neurosurgery, University of Pennsylvania, Philadelphia, PA, USADepartment of Bioengineering, School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, PA, USACenter for Brain Injury and Repair, Department of Neurosurgery, University of Pennsylvania, Philadelphia, PA, USAPenn Nerve Center, University of Pennsylvania, Philadelphia, PA, USAAxonova Medical, LLC, Philadelphia, PA, USATissue engineered nerve grafts (TENGs) built from living neurons and aligned axon tracts offer a revolutionary new approach as “living scaffolds” to bridge major peripheral nerve defects. Clinical application, however, necessitates sufficient shelf-life to allow for shipping from manufacturing facility to clinic as well as storage until use. Here, hypothermic storage in commercially available hibernation media is explored as a potential biopreservation strategy for TENGs. After up to 28 days of refrigeration at 4℃, TENGs maintain viability and structure in vitro . Following transplantation into 1 cm rat sciatic defects, biopreserved TENGs routinely survive and persist for at least 2 weeks and recapitulate pro-regenerative mechanisms of fresh TENGs, including the ability to recruit regenerating host tissue into the graft and extend neurites beyond the margins of the graft. The protocols and timelines established here serve as important foundational work for the manufacturing, storage, and translation of other neuron-based tissue engineered therapeutics.https://doi.org/10.1177/20417314211032488 |
| spellingShingle | Robert B Shultz Kritika S Katiyar Franco A Laimo Justin C Burrell Kevin D Browne Zarina S Ali Daniel K Cullen Biopreservation of living tissue engineered nerve grafts Journal of Tissue Engineering |
| title | Biopreservation of living tissue engineered nerve grafts |
| title_full | Biopreservation of living tissue engineered nerve grafts |
| title_fullStr | Biopreservation of living tissue engineered nerve grafts |
| title_full_unstemmed | Biopreservation of living tissue engineered nerve grafts |
| title_short | Biopreservation of living tissue engineered nerve grafts |
| title_sort | biopreservation of living tissue engineered nerve grafts |
| url | https://doi.org/10.1177/20417314211032488 |
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