Poly-phenolic fraction of Chrysophyllum cainito extract induces cell death in osteosarcoma cells
Osteosarcoma shows resistance to chemotherapy and many side effects. The ethyl acetate fraction of ethanolic extract of Chrysophyllum cainito was taken in the present work. The extract was characterized for its phenolic content, antioxidant capacity (ferric reducing antioxidant power and 2,2-dipheny...
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| Format: | Article |
| Language: | English |
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Bangladesh Pharmacological Society
2015-11-01
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| Series: | Bangladesh Journal of Pharmacology |
| Subjects: | |
| Online Access: | https://www.banglajol.info/index.php/BJP/article/view/24576 |
| _version_ | 1818210709226389504 |
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| author | Liu-Bing Li Subin Lin Jun Yan Qian-Liang Wang Zhi-Ying Fan Qi-Rong Dong Jian-Zhong Qin Zong-Gang Xie |
| author_facet | Liu-Bing Li Subin Lin Jun Yan Qian-Liang Wang Zhi-Ying Fan Qi-Rong Dong Jian-Zhong Qin Zong-Gang Xie |
| author_sort | Liu-Bing Li |
| collection | DOAJ |
| description | Osteosarcoma shows resistance to chemotherapy and many side effects. The ethyl acetate fraction of ethanolic extract of Chrysophyllum cainito was taken in the present work. The extract was characterized for its phenolic content, antioxidant capacity (ferric reducing antioxidant power and 2,2-diphenyl-1-picrylhydrazyl assays), ability to form reactive oxygen species and annexin V assay. The extract contained phenolic content of 30.1 ± 0.2 mg GAE/g extract. Ferric reducing antioxidant power assay and DPPH assay showed 213.2 ± 12.4 mM Fe2+ equivalent per gram extract and 85.6 ± 4.9 TE/g respectively. For production of reactive oxygen species in the osteosarcoma cells, extract at 50 µg GAE/mLconcentration was statistically equivalent to 100 µM H2O2. EC50 of extract was calculated to be 133 µg GAE/mL in cell viability studies. |
| first_indexed | 2024-12-12T05:20:55Z |
| format | Article |
| id | doaj.art-d87483edb1bf4f4faab9cce0a7237756 |
| institution | Directory Open Access Journal |
| issn | 1991-0088 |
| language | English |
| last_indexed | 2024-12-12T05:20:55Z |
| publishDate | 2015-11-01 |
| publisher | Bangladesh Pharmacological Society |
| record_format | Article |
| series | Bangladesh Journal of Pharmacology |
| spelling | doaj.art-d87483edb1bf4f4faab9cce0a72377562022-12-22T00:36:38ZengBangladesh Pharmacological SocietyBangladesh Journal of Pharmacology1991-00882015-11-0110410.3329/bjp.v10i4.24576Poly-phenolic fraction of Chrysophyllum cainito extract induces cell death in osteosarcoma cellsLiu-Bing Li0Subin Lin1Jun Yan2Qian-Liang Wang3Zhi-Ying Fan4Qi-Rong Dong5Jian-Zhong Qin6Zong-Gang Xie7Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004Osteosarcoma shows resistance to chemotherapy and many side effects. The ethyl acetate fraction of ethanolic extract of Chrysophyllum cainito was taken in the present work. The extract was characterized for its phenolic content, antioxidant capacity (ferric reducing antioxidant power and 2,2-diphenyl-1-picrylhydrazyl assays), ability to form reactive oxygen species and annexin V assay. The extract contained phenolic content of 30.1 ± 0.2 mg GAE/g extract. Ferric reducing antioxidant power assay and DPPH assay showed 213.2 ± 12.4 mM Fe2+ equivalent per gram extract and 85.6 ± 4.9 TE/g respectively. For production of reactive oxygen species in the osteosarcoma cells, extract at 50 µg GAE/mLconcentration was statistically equivalent to 100 µM H2O2. EC50 of extract was calculated to be 133 µg GAE/mL in cell viability studies.https://www.banglajol.info/index.php/BJP/article/view/24576Cell deathChrysophyllum cainitoOsteosarcoma cellPoly-phenolic |
| spellingShingle | Liu-Bing Li Subin Lin Jun Yan Qian-Liang Wang Zhi-Ying Fan Qi-Rong Dong Jian-Zhong Qin Zong-Gang Xie Poly-phenolic fraction of Chrysophyllum cainito extract induces cell death in osteosarcoma cells Bangladesh Journal of Pharmacology Cell death Chrysophyllum cainito Osteosarcoma cell Poly-phenolic |
| title | Poly-phenolic fraction of Chrysophyllum cainito extract induces cell death in osteosarcoma cells |
| title_full | Poly-phenolic fraction of Chrysophyllum cainito extract induces cell death in osteosarcoma cells |
| title_fullStr | Poly-phenolic fraction of Chrysophyllum cainito extract induces cell death in osteosarcoma cells |
| title_full_unstemmed | Poly-phenolic fraction of Chrysophyllum cainito extract induces cell death in osteosarcoma cells |
| title_short | Poly-phenolic fraction of Chrysophyllum cainito extract induces cell death in osteosarcoma cells |
| title_sort | poly phenolic fraction of chrysophyllum cainito extract induces cell death in osteosarcoma cells |
| topic | Cell death Chrysophyllum cainito Osteosarcoma cell Poly-phenolic |
| url | https://www.banglajol.info/index.php/BJP/article/view/24576 |
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