Investigating the Bioconversion Potential of Volatile Fatty Acids: Use of Oleaginous Yeasts <i>Rhodosporidium toruloides</i> and <i>Cryptococcus curvatus</i> towards the Sustainable Production of Biodiesel and Odd-Chain Fatty Acids
Oleaginous yeasts have attracted increasing scientific interest as single cell oil (SCO) producers. SCO can be used as a fossil-free fuel substitute, but also as a source of rarely found odd-chain fatty acids (OCFAs), such as C15, C17, and C25 fatty acids which have a wide range of nutritional and b...
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MDPI AG
2022-06-01
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| Online Access: | https://www.mdpi.com/2076-3417/12/13/6541 |
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| author | Eleni Krikigianni Leonidas Matsakas Ulrika Rova Paul Christakopoulos Alok Patel |
| author_facet | Eleni Krikigianni Leonidas Matsakas Ulrika Rova Paul Christakopoulos Alok Patel |
| author_sort | Eleni Krikigianni |
| collection | DOAJ |
| description | Oleaginous yeasts have attracted increasing scientific interest as single cell oil (SCO) producers. SCO can be used as a fossil-free fuel substitute, but also as a source of rarely found odd-chain fatty acids (OCFAs), such as C15, C17, and C25 fatty acids which have a wide range of nutritional and biological applications. Volatile fatty acids (VFAs) have gained interest as sustainable carbon source for yeasts. This study aims to improve current knowledge on yeast species that yield high amounts of SCO using VFAs as a carbon source. Specifically, the growth of the promising yeasts <i>Cryptococcus curvatus</i> and <i>Rhodotorula toruloides</i> was evaluated on individual VFAs, such as acetic, propionic, and butyric acid. <i>C. curvatus</i> proved to be more tolerant in higher concentrations of VFAs (up to 60 g/L), while butyric acid favored biomass and lipid conversion (0.65 and 0.23 g/g<sub>substrate</sub>, respectively). For <i>R. toruloides</i>, butyric acid favored biomass conversion (0.48 g/g<sub>substrate</sub>), but lipid conversion was favored using acetic acid, instead (0.14 g/g<sub>substrate</sub>). Propionic acid induced the formation of OCFAs, which yielded higher amounts for <i>C. curvatus</i> (up to 2.17 g/L). VFAs derived from the anaerobic digestion of brewer’s spent grain were tested as a cost-competitive carbon source and illustrated the significance of the combination of different VFAs in the quality of the produced SCO, by improving the biodiesel properties and OCFAs production. |
| first_indexed | 2024-03-09T22:07:54Z |
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| id | doaj.art-d8773f326f80444d91af26fa4b4b9023 |
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| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-09T22:07:54Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
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| series | Applied Sciences |
| spelling | doaj.art-d8773f326f80444d91af26fa4b4b90232023-11-23T19:38:28ZengMDPI AGApplied Sciences2076-34172022-06-011213654110.3390/app12136541Investigating the Bioconversion Potential of Volatile Fatty Acids: Use of Oleaginous Yeasts <i>Rhodosporidium toruloides</i> and <i>Cryptococcus curvatus</i> towards the Sustainable Production of Biodiesel and Odd-Chain Fatty AcidsEleni Krikigianni0Leonidas Matsakas1Ulrika Rova2Paul Christakopoulos3Alok Patel4Biochemical Process Engineering, Division of Chemical Engineering, Department of Civil, Environmental, and Natural Resources Engineering, Luleå University of Technology, SE-971 87 Luleå, SwedenBiochemical Process Engineering, Division of Chemical Engineering, Department of Civil, Environmental, and Natural Resources Engineering, Luleå University of Technology, SE-971 87 Luleå, SwedenBiochemical Process Engineering, Division of Chemical Engineering, Department of Civil, Environmental, and Natural Resources Engineering, Luleå University of Technology, SE-971 87 Luleå, SwedenBiochemical Process Engineering, Division of Chemical Engineering, Department of Civil, Environmental, and Natural Resources Engineering, Luleå University of Technology, SE-971 87 Luleå, SwedenBiochemical Process Engineering, Division of Chemical Engineering, Department of Civil, Environmental, and Natural Resources Engineering, Luleå University of Technology, SE-971 87 Luleå, SwedenOleaginous yeasts have attracted increasing scientific interest as single cell oil (SCO) producers. SCO can be used as a fossil-free fuel substitute, but also as a source of rarely found odd-chain fatty acids (OCFAs), such as C15, C17, and C25 fatty acids which have a wide range of nutritional and biological applications. Volatile fatty acids (VFAs) have gained interest as sustainable carbon source for yeasts. This study aims to improve current knowledge on yeast species that yield high amounts of SCO using VFAs as a carbon source. Specifically, the growth of the promising yeasts <i>Cryptococcus curvatus</i> and <i>Rhodotorula toruloides</i> was evaluated on individual VFAs, such as acetic, propionic, and butyric acid. <i>C. curvatus</i> proved to be more tolerant in higher concentrations of VFAs (up to 60 g/L), while butyric acid favored biomass and lipid conversion (0.65 and 0.23 g/g<sub>substrate</sub>, respectively). For <i>R. toruloides</i>, butyric acid favored biomass conversion (0.48 g/g<sub>substrate</sub>), but lipid conversion was favored using acetic acid, instead (0.14 g/g<sub>substrate</sub>). Propionic acid induced the formation of OCFAs, which yielded higher amounts for <i>C. curvatus</i> (up to 2.17 g/L). VFAs derived from the anaerobic digestion of brewer’s spent grain were tested as a cost-competitive carbon source and illustrated the significance of the combination of different VFAs in the quality of the produced SCO, by improving the biodiesel properties and OCFAs production.https://www.mdpi.com/2076-3417/12/13/6541biodieselodd-chain fatty acids (OCFAs)oleaginous yeasts (OYs)single cell oil (SCO)volatile fatty acids (VFAs) |
| spellingShingle | Eleni Krikigianni Leonidas Matsakas Ulrika Rova Paul Christakopoulos Alok Patel Investigating the Bioconversion Potential of Volatile Fatty Acids: Use of Oleaginous Yeasts <i>Rhodosporidium toruloides</i> and <i>Cryptococcus curvatus</i> towards the Sustainable Production of Biodiesel and Odd-Chain Fatty Acids Applied Sciences biodiesel odd-chain fatty acids (OCFAs) oleaginous yeasts (OYs) single cell oil (SCO) volatile fatty acids (VFAs) |
| title | Investigating the Bioconversion Potential of Volatile Fatty Acids: Use of Oleaginous Yeasts <i>Rhodosporidium toruloides</i> and <i>Cryptococcus curvatus</i> towards the Sustainable Production of Biodiesel and Odd-Chain Fatty Acids |
| title_full | Investigating the Bioconversion Potential of Volatile Fatty Acids: Use of Oleaginous Yeasts <i>Rhodosporidium toruloides</i> and <i>Cryptococcus curvatus</i> towards the Sustainable Production of Biodiesel and Odd-Chain Fatty Acids |
| title_fullStr | Investigating the Bioconversion Potential of Volatile Fatty Acids: Use of Oleaginous Yeasts <i>Rhodosporidium toruloides</i> and <i>Cryptococcus curvatus</i> towards the Sustainable Production of Biodiesel and Odd-Chain Fatty Acids |
| title_full_unstemmed | Investigating the Bioconversion Potential of Volatile Fatty Acids: Use of Oleaginous Yeasts <i>Rhodosporidium toruloides</i> and <i>Cryptococcus curvatus</i> towards the Sustainable Production of Biodiesel and Odd-Chain Fatty Acids |
| title_short | Investigating the Bioconversion Potential of Volatile Fatty Acids: Use of Oleaginous Yeasts <i>Rhodosporidium toruloides</i> and <i>Cryptococcus curvatus</i> towards the Sustainable Production of Biodiesel and Odd-Chain Fatty Acids |
| title_sort | investigating the bioconversion potential of volatile fatty acids use of oleaginous yeasts i rhodosporidium toruloides i and i cryptococcus curvatus i towards the sustainable production of biodiesel and odd chain fatty acids |
| topic | biodiesel odd-chain fatty acids (OCFAs) oleaginous yeasts (OYs) single cell oil (SCO) volatile fatty acids (VFAs) |
| url | https://www.mdpi.com/2076-3417/12/13/6541 |
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