Synthesis of Magneto-Controllable Polymer Nanocarrier Based on Poly(N-isopropylacrylamide-co-acrylic Acid) for Doxorubicin Immobilization
In this work, the preparation procedure and properties of anionic magnetic microgels loaded with antitumor drug doxorubicin are described. The functional microgels were produced via the in situ formation of iron nanoparticles in an aqueous dispersion of polymer microgels based on poly(N-isopropylacr...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/14/24/5440 |
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| author | Viktoria S. Kusaia Elena Yu. Kozhunova Darya A. Stepanova Vladislava A. Pigareva Andrey V. Sybachin Sergey B. Zezin Anastasiya V. Bolshakova Nikita M. Shchelkunov Evgeny S. Vavaev Evgeny V. Lyubin Andrey A. Fedyanin Vasiliy V. Spiridonov |
| author_facet | Viktoria S. Kusaia Elena Yu. Kozhunova Darya A. Stepanova Vladislava A. Pigareva Andrey V. Sybachin Sergey B. Zezin Anastasiya V. Bolshakova Nikita M. Shchelkunov Evgeny S. Vavaev Evgeny V. Lyubin Andrey A. Fedyanin Vasiliy V. Spiridonov |
| author_sort | Viktoria S. Kusaia |
| collection | DOAJ |
| description | In this work, the preparation procedure and properties of anionic magnetic microgels loaded with antitumor drug doxorubicin are described. The functional microgels were produced via the in situ formation of iron nanoparticles in an aqueous dispersion of polymer microgels based on poly(N-isopropylacrylamide-co-acrylic acid) (PNIPAM-PAA). The composition and morphology of the resulting composite microgels were studied by means of X-ray diffraction, Mössbauer spectroscopy, IR spectroscopy, scanning electron microscopy, atomic-force microscopy, laser microelectrophoresis, and static and dynamic light scattering. The forming nanoparticles were found to be β-FeO(OH). In physiological pH and ionic strength, the obtained composite microgels were shown to possess high colloid stability. The average size of the composites was 200 nm, while the zeta-potential was −27.5 mV. An optical tweezers study has demonstrated the possibility of manipulation with microgel using external magnetic fields. Loading of the composite microgel with doxorubicin did not lead to any change in particle size and colloidal stability. Magnetic-driven interaction of the drug-loaded microgel with model cell membranes was demonstrated by fluorescence microscopy. The described magnetic microgels demonstrate the potential for the controlled delivery of biologically active substances. |
| first_indexed | 2024-03-09T15:56:52Z |
| format | Article |
| id | doaj.art-d877549640144c7aa4244de8cfcf217c |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-09T15:56:52Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-d877549640144c7aa4244de8cfcf217c2023-11-24T17:32:09ZengMDPI AGPolymers2073-43602022-12-011424544010.3390/polym14245440Synthesis of Magneto-Controllable Polymer Nanocarrier Based on Poly(N-isopropylacrylamide-co-acrylic Acid) for Doxorubicin ImmobilizationViktoria S. Kusaia0Elena Yu. Kozhunova1Darya A. Stepanova2Vladislava A. Pigareva3Andrey V. Sybachin4Sergey B. Zezin5Anastasiya V. Bolshakova6Nikita M. Shchelkunov7Evgeny S. Vavaev8Evgeny V. Lyubin9Andrey A. Fedyanin10Vasiliy V. Spiridonov11Department of Chemistry, Lomonosov Moscow State University, Leninskie Gory 1-2, 119234 Moscow, RussiaDepartment of Chemistry, Lomonosov Moscow State University, Leninskie Gory 1-2, 119234 Moscow, RussiaDepartment of Chemistry, Lomonosov Moscow State University, Leninskie Gory 1-2, 119234 Moscow, RussiaDepartment of Chemistry, Lomonosov Moscow State University, Leninskie Gory 1-2, 119234 Moscow, RussiaDepartment of Chemistry, Lomonosov Moscow State University, Leninskie Gory 1-2, 119234 Moscow, RussiaDepartment of Chemistry, Lomonosov Moscow State University, Leninskie Gory 1-2, 119234 Moscow, RussiaDepartment of Chemistry, Lomonosov Moscow State University, Leninskie Gory 1-2, 119234 Moscow, RussiaDepartment of Physics, Lomonosov Moscow State University, Leninskie Gory 1-3, 119991 Moscow, RussiaDepartment of Physics, Lomonosov Moscow State University, Leninskie Gory 1-3, 119991 Moscow, RussiaDepartment of Physics, Lomonosov Moscow State University, Leninskie Gory 1-3, 119991 Moscow, RussiaDepartment of Physics, Lomonosov Moscow State University, Leninskie Gory 1-3, 119991 Moscow, RussiaDepartment of Chemistry, Lomonosov Moscow State University, Leninskie Gory 1-2, 119234 Moscow, RussiaIn this work, the preparation procedure and properties of anionic magnetic microgels loaded with antitumor drug doxorubicin are described. The functional microgels were produced via the in situ formation of iron nanoparticles in an aqueous dispersion of polymer microgels based on poly(N-isopropylacrylamide-co-acrylic acid) (PNIPAM-PAA). The composition and morphology of the resulting composite microgels were studied by means of X-ray diffraction, Mössbauer spectroscopy, IR spectroscopy, scanning electron microscopy, atomic-force microscopy, laser microelectrophoresis, and static and dynamic light scattering. The forming nanoparticles were found to be β-FeO(OH). In physiological pH and ionic strength, the obtained composite microgels were shown to possess high colloid stability. The average size of the composites was 200 nm, while the zeta-potential was −27.5 mV. An optical tweezers study has demonstrated the possibility of manipulation with microgel using external magnetic fields. Loading of the composite microgel with doxorubicin did not lead to any change in particle size and colloidal stability. Magnetic-driven interaction of the drug-loaded microgel with model cell membranes was demonstrated by fluorescence microscopy. The described magnetic microgels demonstrate the potential for the controlled delivery of biologically active substances.https://www.mdpi.com/2073-4360/14/24/5440microgelmagnetosensitivitycontrolled deliveryoptical tweezersstatic light scatteringmodel cell membranes |
| spellingShingle | Viktoria S. Kusaia Elena Yu. Kozhunova Darya A. Stepanova Vladislava A. Pigareva Andrey V. Sybachin Sergey B. Zezin Anastasiya V. Bolshakova Nikita M. Shchelkunov Evgeny S. Vavaev Evgeny V. Lyubin Andrey A. Fedyanin Vasiliy V. Spiridonov Synthesis of Magneto-Controllable Polymer Nanocarrier Based on Poly(N-isopropylacrylamide-co-acrylic Acid) for Doxorubicin Immobilization Polymers microgel magnetosensitivity controlled delivery optical tweezers static light scattering model cell membranes |
| title | Synthesis of Magneto-Controllable Polymer Nanocarrier Based on Poly(N-isopropylacrylamide-co-acrylic Acid) for Doxorubicin Immobilization |
| title_full | Synthesis of Magneto-Controllable Polymer Nanocarrier Based on Poly(N-isopropylacrylamide-co-acrylic Acid) for Doxorubicin Immobilization |
| title_fullStr | Synthesis of Magneto-Controllable Polymer Nanocarrier Based on Poly(N-isopropylacrylamide-co-acrylic Acid) for Doxorubicin Immobilization |
| title_full_unstemmed | Synthesis of Magneto-Controllable Polymer Nanocarrier Based on Poly(N-isopropylacrylamide-co-acrylic Acid) for Doxorubicin Immobilization |
| title_short | Synthesis of Magneto-Controllable Polymer Nanocarrier Based on Poly(N-isopropylacrylamide-co-acrylic Acid) for Doxorubicin Immobilization |
| title_sort | synthesis of magneto controllable polymer nanocarrier based on poly n isopropylacrylamide co acrylic acid for doxorubicin immobilization |
| topic | microgel magnetosensitivity controlled delivery optical tweezers static light scattering model cell membranes |
| url | https://www.mdpi.com/2073-4360/14/24/5440 |
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