Saphenous vein phlebotomy alleviates neuroinflammatory response and oxidative stress following traumatic brain injury
Objective: In spite of the great advances in the field of traumatic brain injury (TBI), it still remains as a global health problem. Phlebotomy has been mentioned as an effective treatment for TBI in Persian medicine. This study aimed to evaluate the possible effect of saphenous vein phlebotomy (SVP...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Interdisciplinary Neurosurgery |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214751922001402 |
| _version_ | 1811183753668591616 |
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| author | Reza Vaghebin Mohsen Khalili Sedigheh Amiresmaili Mehrdad Roghani Seyed Saeid Esmaeili Saber Hasan Namdar |
| author_facet | Reza Vaghebin Mohsen Khalili Sedigheh Amiresmaili Mehrdad Roghani Seyed Saeid Esmaeili Saber Hasan Namdar |
| author_sort | Reza Vaghebin |
| collection | DOAJ |
| description | Objective: In spite of the great advances in the field of traumatic brain injury (TBI), it still remains as a global health problem. Phlebotomy has been mentioned as an effective treatment for TBI in Persian medicine. This study aimed to evaluate the possible effect of saphenous vein phlebotomy (SVP) on the alleviation of neuroinflammation and oxidative stress after TBI. Methods: Seventy-two adult male Wistar rats were randomly divided into three main groups: sham, TBI and TBI + SVP group. Animal model of TBI was made through a controlled cortical impactor (CCI) device and SVP treatment was applied 10 min after TBI. Then, neurological function, body temperature, cerebral edema, blood–brain barrier integrity, brain apoptosis, oxidative stress, and inflammation were assessed. Results: Our results indicated that SVP was associated with improvement of neurological scores 24 h post-TBI. In addition, SVP application significantly alleviated proinflammatory cytokines (IL- 1β, IL-17, and TNF-α) and oxidative stress factors as shown by reduction of malondialdehyde (MDA) and elevation of superoxide dismutase (SOD) with no significant effect on glutathione peroxidase (GPx) and nitric oxide (NO) in the brain 6 h after TBI. Besides, it reduced body temperature, attenuated severity of brain edema, and also reduced caspase-3 and number of apoptotic neurons. Conclusion: This study showed that SVP could ameliorate the severity of acute TBI, partly through alleviation of neuroinflammation, oxidative stress, and apoptosis. Further studies are necessary for more understanding the involved mechanism(s). |
| first_indexed | 2024-04-11T09:51:10Z |
| format | Article |
| id | doaj.art-d87cde89350a4ca6b85f4d1cfcdc59ad |
| institution | Directory Open Access Journal |
| issn | 2214-7519 |
| language | English |
| last_indexed | 2024-04-11T09:51:10Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Interdisciplinary Neurosurgery |
| spelling | doaj.art-d87cde89350a4ca6b85f4d1cfcdc59ad2022-12-22T04:30:47ZengElsevierInterdisciplinary Neurosurgery2214-75192022-12-0130101626Saphenous vein phlebotomy alleviates neuroinflammatory response and oxidative stress following traumatic brain injuryReza Vaghebin0Mohsen Khalili1Sedigheh Amiresmaili2Mehrdad Roghani3Seyed Saeid Esmaeili Saber4Hasan Namdar5Department of Traditional Persian Medicine, School of Medicine, Shahed University, Tehran, IranNeurophysiology Research Center, Shahed University, Tehran, IranDepartment of Physiology, Bam University of Medical Sciences, Bam, IranNeurophysiology Research Center, Shahed University, Tehran, IranDepartment of Traditional Persian Medicine, School of Medicine, Shahed University, Tehran, IranTraditional Medicine Clinical Trial Research Center, Shahed University, Tehran, Iran; Department of Iranian Traditional Medicine, School of Medicine, Shahed University, Tehran, Iran; Corresponding author.Objective: In spite of the great advances in the field of traumatic brain injury (TBI), it still remains as a global health problem. Phlebotomy has been mentioned as an effective treatment for TBI in Persian medicine. This study aimed to evaluate the possible effect of saphenous vein phlebotomy (SVP) on the alleviation of neuroinflammation and oxidative stress after TBI. Methods: Seventy-two adult male Wistar rats were randomly divided into three main groups: sham, TBI and TBI + SVP group. Animal model of TBI was made through a controlled cortical impactor (CCI) device and SVP treatment was applied 10 min after TBI. Then, neurological function, body temperature, cerebral edema, blood–brain barrier integrity, brain apoptosis, oxidative stress, and inflammation were assessed. Results: Our results indicated that SVP was associated with improvement of neurological scores 24 h post-TBI. In addition, SVP application significantly alleviated proinflammatory cytokines (IL- 1β, IL-17, and TNF-α) and oxidative stress factors as shown by reduction of malondialdehyde (MDA) and elevation of superoxide dismutase (SOD) with no significant effect on glutathione peroxidase (GPx) and nitric oxide (NO) in the brain 6 h after TBI. Besides, it reduced body temperature, attenuated severity of brain edema, and also reduced caspase-3 and number of apoptotic neurons. Conclusion: This study showed that SVP could ameliorate the severity of acute TBI, partly through alleviation of neuroinflammation, oxidative stress, and apoptosis. Further studies are necessary for more understanding the involved mechanism(s).http://www.sciencedirect.com/science/article/pii/S2214751922001402Traumatic brain injuryNeuroinflammationPersian medicinePhlebotomyBloodletting |
| spellingShingle | Reza Vaghebin Mohsen Khalili Sedigheh Amiresmaili Mehrdad Roghani Seyed Saeid Esmaeili Saber Hasan Namdar Saphenous vein phlebotomy alleviates neuroinflammatory response and oxidative stress following traumatic brain injury Interdisciplinary Neurosurgery Traumatic brain injury Neuroinflammation Persian medicine Phlebotomy Bloodletting |
| title | Saphenous vein phlebotomy alleviates neuroinflammatory response and oxidative stress following traumatic brain injury |
| title_full | Saphenous vein phlebotomy alleviates neuroinflammatory response and oxidative stress following traumatic brain injury |
| title_fullStr | Saphenous vein phlebotomy alleviates neuroinflammatory response and oxidative stress following traumatic brain injury |
| title_full_unstemmed | Saphenous vein phlebotomy alleviates neuroinflammatory response and oxidative stress following traumatic brain injury |
| title_short | Saphenous vein phlebotomy alleviates neuroinflammatory response and oxidative stress following traumatic brain injury |
| title_sort | saphenous vein phlebotomy alleviates neuroinflammatory response and oxidative stress following traumatic brain injury |
| topic | Traumatic brain injury Neuroinflammation Persian medicine Phlebotomy Bloodletting |
| url | http://www.sciencedirect.com/science/article/pii/S2214751922001402 |
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