Comparative evaluation under routine conditions of the nitrate reduction assay, the proportion assay and the MGIT 960 assay for drug susceptibility testing of clinical isolates of Mycobacterium tuberculosis
The performance of the nitrate reductase assay (NRA) was compared with the proportion method (PM) on Lowenstein-Jensen medium and the BACTEC MGIT960 assay under routine conditions using 160 clinical isolates of Mycobacterium tuberculosis with a high proportion of resistant strains. The mean time to...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2012-02-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000100021&lng=en&tlng=en |
| _version_ | 1797756736002588672 |
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| author | Leila de Souza Fonseca Gisele Betzler de Oliveira Vieira Luciana Fonseca Sobral Elizabete Oliveira Ribeiro Anna Grazia Marsico |
| author_facet | Leila de Souza Fonseca Gisele Betzler de Oliveira Vieira Luciana Fonseca Sobral Elizabete Oliveira Ribeiro Anna Grazia Marsico |
| author_sort | Leila de Souza Fonseca |
| collection | DOAJ |
| description | The performance of the nitrate reductase assay (NRA) was compared with the proportion method (PM) on Lowenstein-Jensen medium and the BACTEC MGIT960 assay under routine conditions using 160 clinical isolates of Mycobacterium tuberculosis with a high proportion of resistant strains. The mean time to obtain results was 8.8 days and the overall agreements between NRA and PM and NRA and M960 were 95% and 94%, respectively. NRA was easy to perform and represents a useful tool for the rapid screening of drug-resistant M. tuberculosis strains in low-resource countries. |
| first_indexed | 2024-03-12T18:05:50Z |
| format | Article |
| id | doaj.art-d87cf6904ba04173a9ffcacf4fd41593 |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:05:50Z |
| publishDate | 2012-02-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-d87cf6904ba04173a9ffcacf4fd415932023-08-02T09:28:29ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602012-02-01107114214410.1590/S0074-02762012000100021S0074-02762012000100021Comparative evaluation under routine conditions of the nitrate reduction assay, the proportion assay and the MGIT 960 assay for drug susceptibility testing of clinical isolates of Mycobacterium tuberculosisLeila de Souza Fonseca0Gisele Betzler de Oliveira Vieira1Luciana Fonseca Sobral2Elizabete Oliveira Ribeiro3Anna Grazia Marsico4Complexo Hospitalar Instituto de Doenças do TóraxComplexo Hospitalar Instituto de Doenças do TóraxComplexo Hospitalar Instituto de Doenças do TóraxComplexo Hospitalar Instituto de Doenças do TóraxComplexo Hospitalar Instituto de Doenças do TóraxThe performance of the nitrate reductase assay (NRA) was compared with the proportion method (PM) on Lowenstein-Jensen medium and the BACTEC MGIT960 assay under routine conditions using 160 clinical isolates of Mycobacterium tuberculosis with a high proportion of resistant strains. The mean time to obtain results was 8.8 days and the overall agreements between NRA and PM and NRA and M960 were 95% and 94%, respectively. NRA was easy to perform and represents a useful tool for the rapid screening of drug-resistant M. tuberculosis strains in low-resource countries.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000100021&lng=en&tlng=entuberculosisnitrate reductase assaydrug susceptibility |
| spellingShingle | Leila de Souza Fonseca Gisele Betzler de Oliveira Vieira Luciana Fonseca Sobral Elizabete Oliveira Ribeiro Anna Grazia Marsico Comparative evaluation under routine conditions of the nitrate reduction assay, the proportion assay and the MGIT 960 assay for drug susceptibility testing of clinical isolates of Mycobacterium tuberculosis Memorias do Instituto Oswaldo Cruz tuberculosis nitrate reductase assay drug susceptibility |
| title | Comparative evaluation under routine conditions of the nitrate reduction assay, the proportion assay and the MGIT 960 assay for drug susceptibility testing of clinical isolates of Mycobacterium tuberculosis |
| title_full | Comparative evaluation under routine conditions of the nitrate reduction assay, the proportion assay and the MGIT 960 assay for drug susceptibility testing of clinical isolates of Mycobacterium tuberculosis |
| title_fullStr | Comparative evaluation under routine conditions of the nitrate reduction assay, the proportion assay and the MGIT 960 assay for drug susceptibility testing of clinical isolates of Mycobacterium tuberculosis |
| title_full_unstemmed | Comparative evaluation under routine conditions of the nitrate reduction assay, the proportion assay and the MGIT 960 assay for drug susceptibility testing of clinical isolates of Mycobacterium tuberculosis |
| title_short | Comparative evaluation under routine conditions of the nitrate reduction assay, the proportion assay and the MGIT 960 assay for drug susceptibility testing of clinical isolates of Mycobacterium tuberculosis |
| title_sort | comparative evaluation under routine conditions of the nitrate reduction assay the proportion assay and the mgit 960 assay for drug susceptibility testing of clinical isolates of mycobacterium tuberculosis |
| topic | tuberculosis nitrate reductase assay drug susceptibility |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000100021&lng=en&tlng=en |
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