Studies on oxidized low density lipoproteins. Controlled oxidation and a prostaglandin artifact.
Low density lipoproteins (LDL), isolated by ultracentrifugal flotation, were oxidized (LDLOXID) slowly during dialysis against 0.15 M NaCl and subsequent incubation in 96% air-4% CO2 at 37 degrees C. Butylated hydroxytoluene prevented LDL oxidation. LDL preparations from different sera were oxidized...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1989-02-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520383760 |
| _version_ | 1818741395836370944 |
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| author | H F Zhang W B Davis X S Chen R L Whisler D G Cornwell |
| author_facet | H F Zhang W B Davis X S Chen R L Whisler D G Cornwell |
| author_sort | H F Zhang |
| collection | DOAJ |
| description | Low density lipoproteins (LDL), isolated by ultracentrifugal flotation, were oxidized (LDLOXID) slowly during dialysis against 0.15 M NaCl and subsequent incubation in 96% air-4% CO2 at 37 degrees C. Butylated hydroxytoluene prevented LDL oxidation. LDL preparations from different sera were oxidized at different rates and the degree of lipid peroxidation was controlled by varying the incubation time. Mild oxidation did not alter the electrophoretic mobility of the LDLOXID preparations. LDLOXID contained lipid peroxides in neutral lipids, had increased amounts of lysophosphatidylcholine, and contained a number of complex oxidation products that were generated from the oxidation of free fatty acids. These oxidation products included large amounts of soluble material that cross-reacted with antibodies to PGE2 but not 6-keto-PGF1 alpha. The amount of cross-reacting material was proportional to the degree of lipid peroxidation. Cross-reacting material in LDLOXID preparations was evidently formed from the oxidation of free fatty acids released from LDL, since cross-reacting material was also formed when a synthetic fat emulsion was oxidized in the presence of free arachidonic acid. |
| first_indexed | 2024-12-18T01:55:57Z |
| format | Article |
| id | doaj.art-d87ddc40f7534b3bb615bff9f80ce427 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-18T01:55:57Z |
| publishDate | 1989-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-d87ddc40f7534b3bb615bff9f80ce4272022-12-21T21:24:54ZengElsevierJournal of Lipid Research0022-22751989-02-01302141148Studies on oxidized low density lipoproteins. Controlled oxidation and a prostaglandin artifact.H F Zhang0W B Davis1X S Chen2R L Whisler3D G Cornwell4Department of Physiological Chemistry, Ohio State University, Columbus, 43210.Department of Physiological Chemistry, Ohio State University, Columbus, 43210.Department of Physiological Chemistry, Ohio State University, Columbus, 43210.Department of Physiological Chemistry, Ohio State University, Columbus, 43210.Department of Physiological Chemistry, Ohio State University, Columbus, 43210.Low density lipoproteins (LDL), isolated by ultracentrifugal flotation, were oxidized (LDLOXID) slowly during dialysis against 0.15 M NaCl and subsequent incubation in 96% air-4% CO2 at 37 degrees C. Butylated hydroxytoluene prevented LDL oxidation. LDL preparations from different sera were oxidized at different rates and the degree of lipid peroxidation was controlled by varying the incubation time. Mild oxidation did not alter the electrophoretic mobility of the LDLOXID preparations. LDLOXID contained lipid peroxides in neutral lipids, had increased amounts of lysophosphatidylcholine, and contained a number of complex oxidation products that were generated from the oxidation of free fatty acids. These oxidation products included large amounts of soluble material that cross-reacted with antibodies to PGE2 but not 6-keto-PGF1 alpha. The amount of cross-reacting material was proportional to the degree of lipid peroxidation. Cross-reacting material in LDLOXID preparations was evidently formed from the oxidation of free fatty acids released from LDL, since cross-reacting material was also formed when a synthetic fat emulsion was oxidized in the presence of free arachidonic acid.http://www.sciencedirect.com/science/article/pii/S0022227520383760 |
| spellingShingle | H F Zhang W B Davis X S Chen R L Whisler D G Cornwell Studies on oxidized low density lipoproteins. Controlled oxidation and a prostaglandin artifact. Journal of Lipid Research |
| title | Studies on oxidized low density lipoproteins. Controlled oxidation and a prostaglandin artifact. |
| title_full | Studies on oxidized low density lipoproteins. Controlled oxidation and a prostaglandin artifact. |
| title_fullStr | Studies on oxidized low density lipoproteins. Controlled oxidation and a prostaglandin artifact. |
| title_full_unstemmed | Studies on oxidized low density lipoproteins. Controlled oxidation and a prostaglandin artifact. |
| title_short | Studies on oxidized low density lipoproteins. Controlled oxidation and a prostaglandin artifact. |
| title_sort | studies on oxidized low density lipoproteins controlled oxidation and a prostaglandin artifact |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520383760 |
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