Response of mice and ferrets to a monovalent influenza A (H7N9) split vaccine.
In early spring 2013, the emergence of the influenza A (H7N9) virus in humans in Eastern China raised concerns of a new influenza pandemic. Development of a safe and effective H7N9 influenza vaccine is urgently needed. To this end, we first synthesized the hemagglutinin (HA) and neuraminidase (NA) g...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4061005?pdf=render |
| _version_ | 1818030817213939712 |
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| author | Yueqiang Duan Hongjing Gu Rui Chen Zhongpeng Zhao Liangyan Zhang Li Xing Chengcai Lai Peirui Zhang Zhiwei Li Keming Zhang Zhouhai Wang Shaogeng Zhang Xiliang Wang Penghui Yang |
| author_facet | Yueqiang Duan Hongjing Gu Rui Chen Zhongpeng Zhao Liangyan Zhang Li Xing Chengcai Lai Peirui Zhang Zhiwei Li Keming Zhang Zhouhai Wang Shaogeng Zhang Xiliang Wang Penghui Yang |
| author_sort | Yueqiang Duan |
| collection | DOAJ |
| description | In early spring 2013, the emergence of the influenza A (H7N9) virus in humans in Eastern China raised concerns of a new influenza pandemic. Development of a safe and effective H7N9 influenza vaccine is urgently needed. To this end, we first synthesized the hemagglutinin (HA) and neuraminidase (NA) genes of the influenza A (H7N9) virus A/AnHui/1/2013. Using reverse genetics, we rescued a reassortant virus (H7N9/PR8) that contained the HA and NA genes from wild-type H7N9 and six genes encoding internal proteins from the A/Puerto Rico/8/34 (PR8) virus. Next, the pathogenicity of the reassortant virus was evaluated both in vivo and in vitro. We found that the virus was non-pathogenic in mice and was stable after serial passaging in eggs. Furthermore, we found that a monovalent influenza A (H7N9) split vaccine prepared from the virus was immunogenic in mice and ferrets. When given intramuscularly, the vaccine (two doses of at least 15-µg) completely protected mice from normally lethal wild-type H7N9 virus challenge. In summary, our H7N9 vaccine, developed over a short time, is a potential candidate for further clinical evaluation and human use. |
| first_indexed | 2024-12-10T05:41:36Z |
| format | Article |
| id | doaj.art-d88194f9deba4330ab5f45a1db891cb6 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T05:41:36Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-d88194f9deba4330ab5f45a1db891cb62022-12-22T02:00:17ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0196e9932210.1371/journal.pone.0099322Response of mice and ferrets to a monovalent influenza A (H7N9) split vaccine.Yueqiang DuanHongjing GuRui ChenZhongpeng ZhaoLiangyan ZhangLi XingChengcai LaiPeirui ZhangZhiwei LiKeming ZhangZhouhai WangShaogeng ZhangXiliang WangPenghui YangIn early spring 2013, the emergence of the influenza A (H7N9) virus in humans in Eastern China raised concerns of a new influenza pandemic. Development of a safe and effective H7N9 influenza vaccine is urgently needed. To this end, we first synthesized the hemagglutinin (HA) and neuraminidase (NA) genes of the influenza A (H7N9) virus A/AnHui/1/2013. Using reverse genetics, we rescued a reassortant virus (H7N9/PR8) that contained the HA and NA genes from wild-type H7N9 and six genes encoding internal proteins from the A/Puerto Rico/8/34 (PR8) virus. Next, the pathogenicity of the reassortant virus was evaluated both in vivo and in vitro. We found that the virus was non-pathogenic in mice and was stable after serial passaging in eggs. Furthermore, we found that a monovalent influenza A (H7N9) split vaccine prepared from the virus was immunogenic in mice and ferrets. When given intramuscularly, the vaccine (two doses of at least 15-µg) completely protected mice from normally lethal wild-type H7N9 virus challenge. In summary, our H7N9 vaccine, developed over a short time, is a potential candidate for further clinical evaluation and human use.http://europepmc.org/articles/PMC4061005?pdf=render |
| spellingShingle | Yueqiang Duan Hongjing Gu Rui Chen Zhongpeng Zhao Liangyan Zhang Li Xing Chengcai Lai Peirui Zhang Zhiwei Li Keming Zhang Zhouhai Wang Shaogeng Zhang Xiliang Wang Penghui Yang Response of mice and ferrets to a monovalent influenza A (H7N9) split vaccine. PLoS ONE |
| title | Response of mice and ferrets to a monovalent influenza A (H7N9) split vaccine. |
| title_full | Response of mice and ferrets to a monovalent influenza A (H7N9) split vaccine. |
| title_fullStr | Response of mice and ferrets to a monovalent influenza A (H7N9) split vaccine. |
| title_full_unstemmed | Response of mice and ferrets to a monovalent influenza A (H7N9) split vaccine. |
| title_short | Response of mice and ferrets to a monovalent influenza A (H7N9) split vaccine. |
| title_sort | response of mice and ferrets to a monovalent influenza a h7n9 split vaccine |
| url | http://europepmc.org/articles/PMC4061005?pdf=render |
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