Antibacterial, Antioxidant, and Phytotoxic Potential of Phytosynthesized Silver Nanoparticles Using <i>Elaeagnus umbellata</i> Fruit Extract
Due to its eco-friendliness, cost-effectiveness, ability to be handled safely, and a wide variety of biological activities, the green plant-mediated synthesis of nanoparticles has become increasingly popular. The present work deals with the green synthesis and characterization of silver nanoparticle...
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MDPI AG
2022-09-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/18/5847 |
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| author | Hafsa Zulfiqar Muhammad Shoaib Amjad Ansar Mehmood Ghazala Mustafa Zakia Binish Samiullah Khan Huma Arshad Jarosław Proćków José Manuel Pérez de la Lastra |
| author_facet | Hafsa Zulfiqar Muhammad Shoaib Amjad Ansar Mehmood Ghazala Mustafa Zakia Binish Samiullah Khan Huma Arshad Jarosław Proćków José Manuel Pérez de la Lastra |
| author_sort | Hafsa Zulfiqar |
| collection | DOAJ |
| description | Due to its eco-friendliness, cost-effectiveness, ability to be handled safely, and a wide variety of biological activities, the green plant-mediated synthesis of nanoparticles has become increasingly popular. The present work deals with the green synthesis and characterization of silver nanoparticles (AgNPs) using <i>Elaeagnus umbellata</i> (fruit) and the evaluation of its antibacterial, antioxidant, and phytotoxic activities. For the synthesis of AgNPs, fruit extract was treated with a 4 mM AgNO<sub>3</sub> solution at room temperature, and a color change was observed. In UV-Visible spectroscopy, an absorption peak formation at 456 nm was the sign that AgNPs were present in the reaction solution. Scanning electron microscopy and physicochemical X-ray diffraction were used to characterize AgNPs, which revealed that they were crystalline, spherical, and had an average size of 11.94 ± 7.325 nm. The synthesized AgNPs showed excellent antibacterial activity against <i>Klebsiella pneumoniae</i> (14 mm), <i>Staphylococcus aureus</i> (13.5 mm), <i>Proteus mirabilis</i> (13 mm), and <i>Pseudomonas aeruginosa</i> (12.5 mm), as well as considerable antioxidant activity against DPPH with 69% inhibition at an IC<sub>50</sub> value of 43.38 µg/mL. AgNPs also exhibited a concentration-dependent effect on rice plants. Root and shoot length were found to be positively impacted at all concentrations, i.e., 12.5 µg/mL, 25 µg/mL, 50 µg/mL, and 100 µg/mL. Among these concentrations, the 50 µg/mL concentration of AgNPs was found to be most effective. The plant biomass decreased at higher AgNP exposure levels (i.e., 100 µg/mL), whereas 50 µg/mL caused a significant increase in plant biomass as compared to the control. This study provides an eco-friendly method for the synthesis of AgNPs which can be used for their antibacterial and antioxidant activities and also as growth promoters of crop plants. |
| first_indexed | 2024-03-09T23:02:24Z |
| format | Article |
| id | doaj.art-d883ded5330a4aa2b73cbe146ed22808 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T23:02:24Z |
| publishDate | 2022-09-01 |
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| series | Molecules |
| spelling | doaj.art-d883ded5330a4aa2b73cbe146ed228082023-11-23T17:59:54ZengMDPI AGMolecules1420-30492022-09-012718584710.3390/molecules27185847Antibacterial, Antioxidant, and Phytotoxic Potential of Phytosynthesized Silver Nanoparticles Using <i>Elaeagnus umbellata</i> Fruit ExtractHafsa Zulfiqar0Muhammad Shoaib Amjad1Ansar Mehmood2Ghazala Mustafa3Zakia Binish4Samiullah Khan5Huma Arshad6Jarosław Proćków7José Manuel Pérez de la Lastra8Department of Botany, Women University of Azad Jammu & Kashmir Bagh, Azad Kashmir 12500, PakistanDepartment of Botany, Women University of Azad Jammu & Kashmir Bagh, Azad Kashmir 12500, PakistanDepartment of Botany, University of Poonch Rawlakot, Rawalakot 12350, PakistanDepartment of Plant Sciences, Quaid-i-Azam University, Islamabad 45320, PakistanDepartment of Botany, Women University of Azad Jammu & Kashmir Bagh, Azad Kashmir 12500, PakistanDepartment of Botany, Women University of Azad Jammu & Kashmir Bagh, Azad Kashmir 12500, PakistanDepartment of Plant Sciences, Quaid-i-Azam University, Islamabad 45320, PakistanDepartment of Plant Biology, Institute of Environmental Biology, Wrocław University of Environmental and Life Sciences, Kożuchowska 5b, 51-631 Wrocław, PolandBiotecnología de Macromoléculas, Instituto de Productos Naturales y Agrobiología, (IPNA-CSIC), 38206 San Cristóbal de la Laguna, SpainDue to its eco-friendliness, cost-effectiveness, ability to be handled safely, and a wide variety of biological activities, the green plant-mediated synthesis of nanoparticles has become increasingly popular. The present work deals with the green synthesis and characterization of silver nanoparticles (AgNPs) using <i>Elaeagnus umbellata</i> (fruit) and the evaluation of its antibacterial, antioxidant, and phytotoxic activities. For the synthesis of AgNPs, fruit extract was treated with a 4 mM AgNO<sub>3</sub> solution at room temperature, and a color change was observed. In UV-Visible spectroscopy, an absorption peak formation at 456 nm was the sign that AgNPs were present in the reaction solution. Scanning electron microscopy and physicochemical X-ray diffraction were used to characterize AgNPs, which revealed that they were crystalline, spherical, and had an average size of 11.94 ± 7.325 nm. The synthesized AgNPs showed excellent antibacterial activity against <i>Klebsiella pneumoniae</i> (14 mm), <i>Staphylococcus aureus</i> (13.5 mm), <i>Proteus mirabilis</i> (13 mm), and <i>Pseudomonas aeruginosa</i> (12.5 mm), as well as considerable antioxidant activity against DPPH with 69% inhibition at an IC<sub>50</sub> value of 43.38 µg/mL. AgNPs also exhibited a concentration-dependent effect on rice plants. Root and shoot length were found to be positively impacted at all concentrations, i.e., 12.5 µg/mL, 25 µg/mL, 50 µg/mL, and 100 µg/mL. Among these concentrations, the 50 µg/mL concentration of AgNPs was found to be most effective. The plant biomass decreased at higher AgNP exposure levels (i.e., 100 µg/mL), whereas 50 µg/mL caused a significant increase in plant biomass as compared to the control. This study provides an eco-friendly method for the synthesis of AgNPs which can be used for their antibacterial and antioxidant activities and also as growth promoters of crop plants.https://www.mdpi.com/1420-3049/27/18/5847Ag nanoparticlesantibacterial<i>Elaeagnus umbellata</i>phytotoxic activity |
| spellingShingle | Hafsa Zulfiqar Muhammad Shoaib Amjad Ansar Mehmood Ghazala Mustafa Zakia Binish Samiullah Khan Huma Arshad Jarosław Proćków José Manuel Pérez de la Lastra Antibacterial, Antioxidant, and Phytotoxic Potential of Phytosynthesized Silver Nanoparticles Using <i>Elaeagnus umbellata</i> Fruit Extract Molecules Ag nanoparticles antibacterial <i>Elaeagnus umbellata</i> phytotoxic activity |
| title | Antibacterial, Antioxidant, and Phytotoxic Potential of Phytosynthesized Silver Nanoparticles Using <i>Elaeagnus umbellata</i> Fruit Extract |
| title_full | Antibacterial, Antioxidant, and Phytotoxic Potential of Phytosynthesized Silver Nanoparticles Using <i>Elaeagnus umbellata</i> Fruit Extract |
| title_fullStr | Antibacterial, Antioxidant, and Phytotoxic Potential of Phytosynthesized Silver Nanoparticles Using <i>Elaeagnus umbellata</i> Fruit Extract |
| title_full_unstemmed | Antibacterial, Antioxidant, and Phytotoxic Potential of Phytosynthesized Silver Nanoparticles Using <i>Elaeagnus umbellata</i> Fruit Extract |
| title_short | Antibacterial, Antioxidant, and Phytotoxic Potential of Phytosynthesized Silver Nanoparticles Using <i>Elaeagnus umbellata</i> Fruit Extract |
| title_sort | antibacterial antioxidant and phytotoxic potential of phytosynthesized silver nanoparticles using i elaeagnus umbellata i fruit extract |
| topic | Ag nanoparticles antibacterial <i>Elaeagnus umbellata</i> phytotoxic activity |
| url | https://www.mdpi.com/1420-3049/27/18/5847 |
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