Establishment and characterization of a highly metastatic human osteosarcoma cell line from osteosarcoma lung metastases
OS (Osteosarcoma) is the most common malignant tumor in adolescents, and lung metastasis limits its therapeutic outcome. The present study aimed to establish a highly metastatic human OS cell line directly from lung metastases and characterize its biological functions. In this study, epithelioid tum...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-08-01
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| Series: | Journal of Bone Oncology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212137421000336 |
| _version_ | 1819110703378726912 |
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| author | Zepei Fan Guanyu Huang Jupeng Zhao Wuguo Li Tiao Lin Qiao Su Junqiang Yin Jingnan Shen |
| author_facet | Zepei Fan Guanyu Huang Jupeng Zhao Wuguo Li Tiao Lin Qiao Su Junqiang Yin Jingnan Shen |
| author_sort | Zepei Fan |
| collection | DOAJ |
| description | OS (Osteosarcoma) is the most common malignant tumor in adolescents, and lung metastasis limits its therapeutic outcome. The present study aimed to establish a highly metastatic human OS cell line directly from lung metastases and characterize its biological functions. In this study, epithelioid tumor cells with large nucleo-cytoplasmic ratio and abundant organelles were obtained by the tissue mass adherent and repeated digestion adherent method and named ZOSL-1 cells. ZOSL-1 cells had the potential to proliferate in vitro with a doubling time of 39.28 ± 3.04 h and migrate with or without a matrix. ZOSL-1 cells were tumorigenic in vivo, and had the ability to develop lung metastasis after intratibial injection. ZOSL-1 cells expressed the osteogenic-related genes osteocalcin and osteopontin. In addition, the expression of ZOSL-1 in Fas cell surface death receptor (FAS), CD44 molecule (CD44), GNAS complex locus (GNAS), scavenger receptor class B member 1 (SCARB1), C-X-C motif chemokine receptor 4 (CXCR4), cadherin 11 (CDH11), neurofibromin 2 (NF2) and ezrin (EZR) genes may be related to its transfer efficiency. Taken together, these results indicated the high metastatic capability and important biological functions of ZOSL-1 cells. ZOSL-1 establishment provided a relevant model for the study of osteosarcoma lung metastasis. |
| first_indexed | 2024-12-22T03:45:56Z |
| format | Article |
| id | doaj.art-d888e9e524d94eb2a8d94de26306a57b |
| institution | Directory Open Access Journal |
| issn | 2212-1374 |
| language | English |
| last_indexed | 2024-12-22T03:45:56Z |
| publishDate | 2021-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Bone Oncology |
| spelling | doaj.art-d888e9e524d94eb2a8d94de26306a57b2022-12-21T18:40:09ZengElsevierJournal of Bone Oncology2212-13742021-08-0129100378Establishment and characterization of a highly metastatic human osteosarcoma cell line from osteosarcoma lung metastasesZepei Fan0Guanyu Huang1Jupeng Zhao2Wuguo Li3Tiao Lin4Qiao Su5Junqiang Yin6Jingnan Shen7Department of Musculoskeletal Oncology, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong, ChinaDepartment of Musculoskeletal Oncology, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong, ChinaDepartment of Musculoskeletal Oncology, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong, ChinaDepartment of Animal Experiment Center, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, ChinaDepartment of Musculoskeletal Oncology, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong, China; Guangdong Provincial Key Laboratory of Orthopedics and Traumatology, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong, ChinaDepartment of Animal Experiment Center, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, ChinaDepartment of Musculoskeletal Oncology, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong, China; Guangdong Provincial Key Laboratory of Orthopedics and Traumatology, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong, China; Corresponding authors at: Department of Musculoskeletal Oncology, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, China.Department of Musculoskeletal Oncology, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong, China; Guangdong Provincial Key Laboratory of Orthopedics and Traumatology, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong, China; Corresponding authors at: Department of Musculoskeletal Oncology, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, China.OS (Osteosarcoma) is the most common malignant tumor in adolescents, and lung metastasis limits its therapeutic outcome. The present study aimed to establish a highly metastatic human OS cell line directly from lung metastases and characterize its biological functions. In this study, epithelioid tumor cells with large nucleo-cytoplasmic ratio and abundant organelles were obtained by the tissue mass adherent and repeated digestion adherent method and named ZOSL-1 cells. ZOSL-1 cells had the potential to proliferate in vitro with a doubling time of 39.28 ± 3.04 h and migrate with or without a matrix. ZOSL-1 cells were tumorigenic in vivo, and had the ability to develop lung metastasis after intratibial injection. ZOSL-1 cells expressed the osteogenic-related genes osteocalcin and osteopontin. In addition, the expression of ZOSL-1 in Fas cell surface death receptor (FAS), CD44 molecule (CD44), GNAS complex locus (GNAS), scavenger receptor class B member 1 (SCARB1), C-X-C motif chemokine receptor 4 (CXCR4), cadherin 11 (CDH11), neurofibromin 2 (NF2) and ezrin (EZR) genes may be related to its transfer efficiency. Taken together, these results indicated the high metastatic capability and important biological functions of ZOSL-1 cells. ZOSL-1 establishment provided a relevant model for the study of osteosarcoma lung metastasis.http://www.sciencedirect.com/science/article/pii/S2212137421000336ZOSL-1OsteosarcomaPulmonary metastasis |
| spellingShingle | Zepei Fan Guanyu Huang Jupeng Zhao Wuguo Li Tiao Lin Qiao Su Junqiang Yin Jingnan Shen Establishment and characterization of a highly metastatic human osteosarcoma cell line from osteosarcoma lung metastases Journal of Bone Oncology ZOSL-1 Osteosarcoma Pulmonary metastasis |
| title | Establishment and characterization of a highly metastatic human osteosarcoma cell line from osteosarcoma lung metastases |
| title_full | Establishment and characterization of a highly metastatic human osteosarcoma cell line from osteosarcoma lung metastases |
| title_fullStr | Establishment and characterization of a highly metastatic human osteosarcoma cell line from osteosarcoma lung metastases |
| title_full_unstemmed | Establishment and characterization of a highly metastatic human osteosarcoma cell line from osteosarcoma lung metastases |
| title_short | Establishment and characterization of a highly metastatic human osteosarcoma cell line from osteosarcoma lung metastases |
| title_sort | establishment and characterization of a highly metastatic human osteosarcoma cell line from osteosarcoma lung metastases |
| topic | ZOSL-1 Osteosarcoma Pulmonary metastasis |
| url | http://www.sciencedirect.com/science/article/pii/S2212137421000336 |
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