THE RAPID CULTURE METHOD FOR THE INDICATION OF RABIES VIRUS ANTIGEN IN INFECTED CELL CULTURES
Abstract. The rapid culture method for the indication of antigens of rabies virus has been improved. It allows to detect the antigen of rabies virus within 5 hours from the moment the cell cultures was infected. To achieve this aim it is necessary to use cell culture BHK-F, polyclonal antirabies FIT...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | Russian |
| Published: |
Sankt-Peterburg : NIIÈM imeni Pastera
2014-07-01
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| Series: | Инфекция и иммунитет |
| Subjects: | |
| Online Access: | https://www.iimmun.ru/iimm/article/view/140 |
| Summary: | Abstract. The rapid culture method for the indication of antigens of rabies virus has been improved. It allows to detect the antigen of rabies virus within 5 hours from the moment the cell cultures was infected. To achieve this aim it is necessary to use cell culture BHK-F, polyclonal antirabies FITC labelled immunoglobulin and direct method of membrane immunofluorescense. Applying of twofold freshly filtrated 3% paraformaldehyde as fixing solution allows to detect early virus proteins of rabies virus. |
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| ISSN: | 2220-7619 2313-7398 |