Dynamics of alkannin/shikonin biosynthesis in response to jasmonate and salicylic acid in Lithospermum officinale
Abstract Alkannin/shikonin and their derivatives are specialised metabolites of high pharmaceutical and ecological importance exclusively produced in the periderm of members of the plant family Boraginaceae. Previous studies have shown that their biosynthesis is induced in response to methyl jasmona...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-21322-0 |
| _version_ | 1828723317212708864 |
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| author | Muhammad Ahmad Alicia Varela Alonso Antigoni E. Koletti Nebojša Rodić Michael Reichelt Philipp Rödel Andreana N. Assimopoulou Ovidiu Paun Stéphane Declerck Carolin Schneider Eva M. Molin |
| author_facet | Muhammad Ahmad Alicia Varela Alonso Antigoni E. Koletti Nebojša Rodić Michael Reichelt Philipp Rödel Andreana N. Assimopoulou Ovidiu Paun Stéphane Declerck Carolin Schneider Eva M. Molin |
| author_sort | Muhammad Ahmad |
| collection | DOAJ |
| description | Abstract Alkannin/shikonin and their derivatives are specialised metabolites of high pharmaceutical and ecological importance exclusively produced in the periderm of members of the plant family Boraginaceae. Previous studies have shown that their biosynthesis is induced in response to methyl jasmonate but not salicylic acid, two phytohormones that play important roles in plant defence. However, mechanistic understanding of induction and non-induction remains largely unknown. In the present study, we generated the first comprehensive transcriptomic dataset and metabolite profiles of Lithospermum officinale plants treated with methyl jasmonate and salicylic acid to shed light on the underlying mechanisms. Our results highlight the diverse biological processes activated by both phytohormones and reveal the important regulatory role of the mevalonate pathway in alkannin/shikonin biosynthesis in L. officinale. Furthermore, by modelling a coexpression network, we uncovered structural and novel regulatory candidate genes connected to alkannin/shikonin biosynthesis. Besides providing new mechanistic insights into alkannin/shikonin biosynthesis, the generated methyl jasmonate and salicylic acid elicited expression profiles together with the coexpression networks serve as important functional genomic resources for the scientific community aiming at deepening the understanding of alkannin/shikonin biosynthesis. |
| first_indexed | 2024-04-12T12:47:41Z |
| format | Article |
| id | doaj.art-d890f692b6e0414db49122ca0c45fcf4 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-12T12:47:41Z |
| publishDate | 2022-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-d890f692b6e0414db49122ca0c45fcf42022-12-22T03:32:34ZengNature PortfolioScientific Reports2045-23222022-10-0112111710.1038/s41598-022-21322-0Dynamics of alkannin/shikonin biosynthesis in response to jasmonate and salicylic acid in Lithospermum officinaleMuhammad Ahmad0Alicia Varela Alonso1Antigoni E. Koletti2Nebojša Rodić3Michael Reichelt4Philipp Rödel5Andreana N. Assimopoulou6Ovidiu Paun7Stéphane Declerck8Carolin Schneider9Eva M. Molin10Center for Health & Bioresources, AIT Austrian Institute of Technology GmbHInstitut für Pflanzenkultur GmbH & Co. KG.Department of Chemical Engineering, Laboratory of Organic Chemistry and Center of Interdisciplinary Research and Innovation, Natural Products Research Centre of Excellence (NatPro-AUTh), Aristotle University of ThessalonikiDepartment of Chemical Engineering, Laboratory of Organic Chemistry and Center of Interdisciplinary Research and Innovation, Natural Products Research Centre of Excellence (NatPro-AUTh), Aristotle University of ThessalonikiDepartment of Biochemistry, Max Planck Institute for Chemical EcologyInstitut für Pflanzenkultur GmbH & Co. KG.Department of Chemical Engineering, Laboratory of Organic Chemistry and Center of Interdisciplinary Research and Innovation, Natural Products Research Centre of Excellence (NatPro-AUTh), Aristotle University of ThessalonikiDepartment of Botany and Biodiversity Research, University of ViennaEarth and Life Institute, Université Catholique de LouvainInstitut für Pflanzenkultur GmbH & Co. KG.Center for Health & Bioresources, AIT Austrian Institute of Technology GmbHAbstract Alkannin/shikonin and their derivatives are specialised metabolites of high pharmaceutical and ecological importance exclusively produced in the periderm of members of the plant family Boraginaceae. Previous studies have shown that their biosynthesis is induced in response to methyl jasmonate but not salicylic acid, two phytohormones that play important roles in plant defence. However, mechanistic understanding of induction and non-induction remains largely unknown. In the present study, we generated the first comprehensive transcriptomic dataset and metabolite profiles of Lithospermum officinale plants treated with methyl jasmonate and salicylic acid to shed light on the underlying mechanisms. Our results highlight the diverse biological processes activated by both phytohormones and reveal the important regulatory role of the mevalonate pathway in alkannin/shikonin biosynthesis in L. officinale. Furthermore, by modelling a coexpression network, we uncovered structural and novel regulatory candidate genes connected to alkannin/shikonin biosynthesis. Besides providing new mechanistic insights into alkannin/shikonin biosynthesis, the generated methyl jasmonate and salicylic acid elicited expression profiles together with the coexpression networks serve as important functional genomic resources for the scientific community aiming at deepening the understanding of alkannin/shikonin biosynthesis.https://doi.org/10.1038/s41598-022-21322-0 |
| spellingShingle | Muhammad Ahmad Alicia Varela Alonso Antigoni E. Koletti Nebojša Rodić Michael Reichelt Philipp Rödel Andreana N. Assimopoulou Ovidiu Paun Stéphane Declerck Carolin Schneider Eva M. Molin Dynamics of alkannin/shikonin biosynthesis in response to jasmonate and salicylic acid in Lithospermum officinale Scientific Reports |
| title | Dynamics of alkannin/shikonin biosynthesis in response to jasmonate and salicylic acid in Lithospermum officinale |
| title_full | Dynamics of alkannin/shikonin biosynthesis in response to jasmonate and salicylic acid in Lithospermum officinale |
| title_fullStr | Dynamics of alkannin/shikonin biosynthesis in response to jasmonate and salicylic acid in Lithospermum officinale |
| title_full_unstemmed | Dynamics of alkannin/shikonin biosynthesis in response to jasmonate and salicylic acid in Lithospermum officinale |
| title_short | Dynamics of alkannin/shikonin biosynthesis in response to jasmonate and salicylic acid in Lithospermum officinale |
| title_sort | dynamics of alkannin shikonin biosynthesis in response to jasmonate and salicylic acid in lithospermum officinale |
| url | https://doi.org/10.1038/s41598-022-21322-0 |
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