Alginate-Based Patch for Middle Ear Delivery of Probiotics: A Preliminary Study Using Electrospray and Electrospinning
Antimicrobial resistance poses a growing challenge in respiratory tract diseases like otitis media, often necessitating surgical interventions due to pharmacological treatment limitations. Bacteriotherapy, involving probiotics and/or their bioproducts, emerges as a promising alternative in such a sc...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/13/23/12750 |
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| author | Beatrice Cecchini Roberta Rovelli Lorenzo Zavagna Bahareh Azimi Teresa Macchi Esingül Kaya Semih Esin Luca Bruschini Mario Milazzo Giovanna Batoni Serena Danti |
| author_facet | Beatrice Cecchini Roberta Rovelli Lorenzo Zavagna Bahareh Azimi Teresa Macchi Esingül Kaya Semih Esin Luca Bruschini Mario Milazzo Giovanna Batoni Serena Danti |
| author_sort | Beatrice Cecchini |
| collection | DOAJ |
| description | Antimicrobial resistance poses a growing challenge in respiratory tract diseases like otitis media, often necessitating surgical interventions due to pharmacological treatment limitations. Bacteriotherapy, involving probiotics and/or their bioproducts, emerges as a promising alternative in such a scenario. This study aims to pave the way to middle ear bacteriotherapy by developing an innovative sodium alginate (SA)-based probiotic delivery system using electrospinning and electrospray techniques. Electrospray enabled the precise production of probiotic-laden SA microparticles, demonstrating potential for targeted bacterial delivery. By overcoming challenges due to the SA molecular structure, we successfully electrospun SA-based fiber meshes with poly(ethylene oxide) (PEO) as a support polymer. The rheologic behavior of the probiotic/SA solutions and the morphology of the obtained microparticles and fibers was evaluated, along with the diameter variation over time. The cytocompatibility of the produced microparticles and fibers was assessed using human dermal keratinocytes and their antimicrobial activity was tested against <i>E. coli</i>. The incorporation of probiotic-laden SA microparticles within electrospun SA/PEO fiber meshes finally offered a patch-like structure to be applied on the tympanic membrane or on the outer auditory canal, which could be a versatile and ideally safe treatment strategy in chronic otitis media. This innovative approach holds promise for clinical applications dealing with inflammatory processes, infections and dysbiosis, thus possibly addressing the complex healing process of chronic upper respiratory diseases while mitigating antimicrobial resistance. |
| first_indexed | 2024-03-08T13:09:39Z |
| format | Article |
| id | doaj.art-d89217f0612a440eb4ed63869e95d02e |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-08T13:09:39Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-d89217f0612a440eb4ed63869e95d02e2024-01-18T15:50:33ZengMDPI AGApplied Sciences2076-34172023-11-0113231275010.3390/app132312750Alginate-Based Patch for Middle Ear Delivery of Probiotics: A Preliminary Study Using Electrospray and ElectrospinningBeatrice Cecchini0Roberta Rovelli1Lorenzo Zavagna2Bahareh Azimi3Teresa Macchi4Esingül Kaya5Semih Esin6Luca Bruschini7Mario Milazzo8Giovanna Batoni9Serena Danti10Department of Civil and Industrial Engineering, University of Pisa, 56122 Pisa, ItalyDepartment of Civil and Industrial Engineering, University of Pisa, 56122 Pisa, ItalyPEGASO Doctoral School of Life Sciences, University of Siena, 53100 Siena, ItalyDepartment of Civil and Industrial Engineering, University of Pisa, 56122 Pisa, ItalyDepartment of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, 56126 Pisa, ItalyDepartment of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, 56126 Pisa, ItalyDepartment of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, 56126 Pisa, ItalyDepartment of Surgical, Medical, Molecular Pathology and Emergency Medicine, University of Pisa, 56126 Pisa, ItalyDepartment of Civil and Industrial Engineering, University of Pisa, 56122 Pisa, ItalyDepartment of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, 56126 Pisa, ItalyDepartment of Civil and Industrial Engineering, University of Pisa, 56122 Pisa, ItalyAntimicrobial resistance poses a growing challenge in respiratory tract diseases like otitis media, often necessitating surgical interventions due to pharmacological treatment limitations. Bacteriotherapy, involving probiotics and/or their bioproducts, emerges as a promising alternative in such a scenario. This study aims to pave the way to middle ear bacteriotherapy by developing an innovative sodium alginate (SA)-based probiotic delivery system using electrospinning and electrospray techniques. Electrospray enabled the precise production of probiotic-laden SA microparticles, demonstrating potential for targeted bacterial delivery. By overcoming challenges due to the SA molecular structure, we successfully electrospun SA-based fiber meshes with poly(ethylene oxide) (PEO) as a support polymer. The rheologic behavior of the probiotic/SA solutions and the morphology of the obtained microparticles and fibers was evaluated, along with the diameter variation over time. The cytocompatibility of the produced microparticles and fibers was assessed using human dermal keratinocytes and their antimicrobial activity was tested against <i>E. coli</i>. The incorporation of probiotic-laden SA microparticles within electrospun SA/PEO fiber meshes finally offered a patch-like structure to be applied on the tympanic membrane or on the outer auditory canal, which could be a versatile and ideally safe treatment strategy in chronic otitis media. This innovative approach holds promise for clinical applications dealing with inflammatory processes, infections and dysbiosis, thus possibly addressing the complex healing process of chronic upper respiratory diseases while mitigating antimicrobial resistance.https://www.mdpi.com/2076-3417/13/23/12750additive manufacturingbacteriotherapyfiber meshesencapsulationeardrum |
| spellingShingle | Beatrice Cecchini Roberta Rovelli Lorenzo Zavagna Bahareh Azimi Teresa Macchi Esingül Kaya Semih Esin Luca Bruschini Mario Milazzo Giovanna Batoni Serena Danti Alginate-Based Patch for Middle Ear Delivery of Probiotics: A Preliminary Study Using Electrospray and Electrospinning Applied Sciences additive manufacturing bacteriotherapy fiber meshes encapsulation eardrum |
| title | Alginate-Based Patch for Middle Ear Delivery of Probiotics: A Preliminary Study Using Electrospray and Electrospinning |
| title_full | Alginate-Based Patch for Middle Ear Delivery of Probiotics: A Preliminary Study Using Electrospray and Electrospinning |
| title_fullStr | Alginate-Based Patch for Middle Ear Delivery of Probiotics: A Preliminary Study Using Electrospray and Electrospinning |
| title_full_unstemmed | Alginate-Based Patch for Middle Ear Delivery of Probiotics: A Preliminary Study Using Electrospray and Electrospinning |
| title_short | Alginate-Based Patch for Middle Ear Delivery of Probiotics: A Preliminary Study Using Electrospray and Electrospinning |
| title_sort | alginate based patch for middle ear delivery of probiotics a preliminary study using electrospray and electrospinning |
| topic | additive manufacturing bacteriotherapy fiber meshes encapsulation eardrum |
| url | https://www.mdpi.com/2076-3417/13/23/12750 |
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