Extracts and semi-purified fractions of Tagetes patula flowers in the control of root-knot nematodes
The genus Tagetes constitutes a group of antagonistic plant species that are cytotoxic against plant pathogenic nematodes, with T. patula being particularly efficient. The aim of this study was to evaluate the in vitro effect of extracts and semi-purified fractions of T. patula flowers on eggs and s...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Universidade Estadual de Londrina
2017-11-01
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| Series: | Semina: Ciências Agrárias |
| Subjects: | |
| Online Access: | http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/30092 |
| _version_ | 1818958228688470016 |
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| author | Vanessa Marquito Munhoz Fernando Cesar Baida Gisely Cristiny Lopes Debora Cristina Santiago José Roberto Pinto de Souza João Carlos Palazzo de Mello |
| author_facet | Vanessa Marquito Munhoz Fernando Cesar Baida Gisely Cristiny Lopes Debora Cristina Santiago José Roberto Pinto de Souza João Carlos Palazzo de Mello |
| author_sort | Vanessa Marquito Munhoz |
| collection | DOAJ |
| description | The genus Tagetes constitutes a group of antagonistic plant species that are cytotoxic against plant pathogenic nematodes, with T. patula being particularly efficient. The aim of this study was to evaluate the in vitro effect of extracts and semi-purified fractions of T. patula flowers on eggs and second-stage juveniles (J2) of Meloidogyne incognita, M. javanica, and M. paranaensis, as well as to verify the nematicidal effect of the flavonoids present in T. patula flowers. Extracts and semi-purified fractions were obtained from dried T. patula flowers after maceration and liquidification, yielding crude aqueous (CAE) and crude ethanol-water (CEWE) extracts. Dried flowers were also treated with n-hexane to obtain a crude defatted aqueous extract (CDAE) and a crude ethanol-water defatted extract (CEWDE). Then, the CEWE was fractionated, and the ethyl acetate (EAF), ethanol (EF), methanol (MF), and ethanol:water (EWF) fractions were obtained. CAE, CEWE, CDAE, and CEWDE were tested to evaluate their effects on hatching, mobility, and mortality of J2 of M. incognita, M. javanica, and M. paranaensis. EAF, EF, MF, and EWF fractions were tested on the same variables of M. incognita. All extracts significantly reduced J2 hatching of M. incognita, M. javanica, and M. paranaensis when compared to water and water + DMSO. CEWE had nematicidal effects on the three evaluated species, whereas CEWDE demonstrated nematicidal effects against M. incognita and M. javanica, and nematostatic effects on M. paranaensis. This toxic effect showed by CEWE may be related to the high content of quercetin, a major substance present in this sample. It was also observed that EAF accentuated the nematicidal response on Meloidogyne spp., suggesting that other medium polarity (methoxylated) flavonoids act as nematotoxic substances. Thus, these results suggest that quercetin contributes significantly to the nematicidal activity of CEWE and EAF. |
| first_indexed | 2024-12-20T11:22:25Z |
| format | Article |
| id | doaj.art-d8939c5973b744a1aa12571efe677f37 |
| institution | Directory Open Access Journal |
| issn | 1676-546X 1679-0359 |
| language | English |
| last_indexed | 2024-12-20T11:22:25Z |
| publishDate | 2017-11-01 |
| publisher | Universidade Estadual de Londrina |
| record_format | Article |
| series | Semina: Ciências Agrárias |
| spelling | doaj.art-d8939c5973b744a1aa12571efe677f372022-12-21T19:42:29ZengUniversidade Estadual de LondrinaSemina: Ciências Agrárias1676-546X1679-03592017-11-013863529353810.5433/1679-0359.2017v38n6p352916450Extracts and semi-purified fractions of Tagetes patula flowers in the control of root-knot nematodesVanessa Marquito Munhoz0Fernando Cesar Baida1Gisely Cristiny Lopes2Debora Cristina Santiago3José Roberto Pinto de Souza4João Carlos Palazzo de Mello5Universidade Estadual de MaringáUniversidade Estadual de LondrinaUniversidade Estadual de MaringáUniversidade Estadual de LondrinaUniversidade Estadual de LondrinaUniversidade Estadual de MaringáThe genus Tagetes constitutes a group of antagonistic plant species that are cytotoxic against plant pathogenic nematodes, with T. patula being particularly efficient. The aim of this study was to evaluate the in vitro effect of extracts and semi-purified fractions of T. patula flowers on eggs and second-stage juveniles (J2) of Meloidogyne incognita, M. javanica, and M. paranaensis, as well as to verify the nematicidal effect of the flavonoids present in T. patula flowers. Extracts and semi-purified fractions were obtained from dried T. patula flowers after maceration and liquidification, yielding crude aqueous (CAE) and crude ethanol-water (CEWE) extracts. Dried flowers were also treated with n-hexane to obtain a crude defatted aqueous extract (CDAE) and a crude ethanol-water defatted extract (CEWDE). Then, the CEWE was fractionated, and the ethyl acetate (EAF), ethanol (EF), methanol (MF), and ethanol:water (EWF) fractions were obtained. CAE, CEWE, CDAE, and CEWDE were tested to evaluate their effects on hatching, mobility, and mortality of J2 of M. incognita, M. javanica, and M. paranaensis. EAF, EF, MF, and EWF fractions were tested on the same variables of M. incognita. All extracts significantly reduced J2 hatching of M. incognita, M. javanica, and M. paranaensis when compared to water and water + DMSO. CEWE had nematicidal effects on the three evaluated species, whereas CEWDE demonstrated nematicidal effects against M. incognita and M. javanica, and nematostatic effects on M. paranaensis. This toxic effect showed by CEWE may be related to the high content of quercetin, a major substance present in this sample. It was also observed that EAF accentuated the nematicidal response on Meloidogyne spp., suggesting that other medium polarity (methoxylated) flavonoids act as nematotoxic substances. Thus, these results suggest that quercetin contributes significantly to the nematicidal activity of CEWE and EAF.http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/30092Atividade nematicidaFlavonoidesFracionamento químicoMeloidogyne. |
| spellingShingle | Vanessa Marquito Munhoz Fernando Cesar Baida Gisely Cristiny Lopes Debora Cristina Santiago José Roberto Pinto de Souza João Carlos Palazzo de Mello Extracts and semi-purified fractions of Tagetes patula flowers in the control of root-knot nematodes Semina: Ciências Agrárias Atividade nematicida Flavonoides Fracionamento químico Meloidogyne. |
| title | Extracts and semi-purified fractions of Tagetes patula flowers in the control of root-knot nematodes |
| title_full | Extracts and semi-purified fractions of Tagetes patula flowers in the control of root-knot nematodes |
| title_fullStr | Extracts and semi-purified fractions of Tagetes patula flowers in the control of root-knot nematodes |
| title_full_unstemmed | Extracts and semi-purified fractions of Tagetes patula flowers in the control of root-knot nematodes |
| title_short | Extracts and semi-purified fractions of Tagetes patula flowers in the control of root-knot nematodes |
| title_sort | extracts and semi purified fractions of tagetes patula flowers in the control of root knot nematodes |
| topic | Atividade nematicida Flavonoides Fracionamento químico Meloidogyne. |
| url | http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/30092 |
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