A Clearance Period after Soluble Lead Nanoparticle Inhalation Did Not Ameliorate the Negative Effects on Target Tissues Due to Decreased Immune Response
The inhalation of metal (including lead) nanoparticles poses a real health issue to people and animals living in polluted and/or industrial areas. In this study, we exposed mice to lead(II) nitrate nanoparticles [Pb(NO<sub>3</sub>)<sub>2</sub> NPs], which represent a highly s...
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MDPI AG
2020-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/22/8738 |
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| author | Jana Dumková Tereza Smutná Lucie Vrlíková Bohumil Dočekal Daniela Kristeková Zbyněk Večeřa Zuzana Husáková Veronika Jakešová Adriena Jedličková Pavel Mikuška Lukáš Alexa Pavel Coufalík Michaela Tvrdoňová Kamil Křůmal Tomáš Vaculovič Viktor Kanický Aleš Hampl Marcela Buchtová |
| author_facet | Jana Dumková Tereza Smutná Lucie Vrlíková Bohumil Dočekal Daniela Kristeková Zbyněk Večeřa Zuzana Husáková Veronika Jakešová Adriena Jedličková Pavel Mikuška Lukáš Alexa Pavel Coufalík Michaela Tvrdoňová Kamil Křůmal Tomáš Vaculovič Viktor Kanický Aleš Hampl Marcela Buchtová |
| author_sort | Jana Dumková |
| collection | DOAJ |
| description | The inhalation of metal (including lead) nanoparticles poses a real health issue to people and animals living in polluted and/or industrial areas. In this study, we exposed mice to lead(II) nitrate nanoparticles [Pb(NO<sub>3</sub>)<sub>2</sub> NPs], which represent a highly soluble form of lead, by inhalation. We aimed to uncover the effects of their exposure on individual target organs and to reveal potential variability in the lead clearance. We examined (i) lead biodistribution in target organs using laser ablation and inductively coupled plasma mass spectrometry (LA-ICP-MS) and atomic absorption spectrometry (AAS), (ii) lead effect on histopathological changes and immune cells response in secondary target organs and (iii) the clearance ability of target organs. In the lungs and liver, Pb(NO<sub>3</sub>)<sub>2</sub> NP inhalation induced serious structural changes and their damage was present even after a 5-week clearance period despite the lead having been almost completely eliminated from the tissues. The numbers of macrophages significantly decreased after 11-week Pb(NO<sub>3</sub>)<sub>2</sub> NP inhalation; conversely, abundance of alpha-smooth muscle actin (α-SMA)-positive cells, which are responsible for augmented collagen production, increased in both tissues. Moreover, the expression of nuclear factor κB (<i>NF-κB</i>) and selected cytokines, such as tumor necrosis factor alpha (<i>TNFα</i>), transforming growth factor beta 1 (<i>TGFβ1</i>), interleukin 6(<i>IL-6</i>), <i>IL-1α</i> and <i>IL-1β</i> , displayed a tissue-specific response to lead exposure. In summary, diminished inflammatory response in tissues after Pb(NO<sub>3</sub>)<sub>2</sub> NPs inhalation was associated with prolonged negative effect of lead on tissues, as demonstrated by sustained pathological changes in target organs, even after long clearance period. |
| first_indexed | 2024-03-10T14:44:18Z |
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| id | doaj.art-d89bda6d7d5f4dccbd11629e4f763e01 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T14:44:18Z |
| publishDate | 2020-11-01 |
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| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-d89bda6d7d5f4dccbd11629e4f763e012023-11-20T21:31:42ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-11-012122873810.3390/ijms21228738A Clearance Period after Soluble Lead Nanoparticle Inhalation Did Not Ameliorate the Negative Effects on Target Tissues Due to Decreased Immune ResponseJana Dumková0Tereza Smutná1Lucie Vrlíková2Bohumil Dočekal3Daniela Kristeková4Zbyněk Večeřa5Zuzana Husáková6Veronika Jakešová7Adriena Jedličková8Pavel Mikuška9Lukáš Alexa10Pavel Coufalík11Michaela Tvrdoňová12Kamil Křůmal13Tomáš Vaculovič14Viktor Kanický15Aleš Hampl16Marcela Buchtová17Department of Histology and Embryology, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech RepublicDepartment of Histology and Embryology, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech RepublicLaboratory of Molecular Morphogenesis, Institute of Animal Physiology and Genetics, v.v.i., Czech Academy of Sciences, 602 00 Brno, Czech RepublicDepartment of Environmental Analytical Chemistry, Institute of Analytical Chemistry, v.v.i., Czech Academy of Sciences, 602 00 Brno, Czech RepublicLaboratory of Molecular Morphogenesis, Institute of Animal Physiology and Genetics, v.v.i., Czech Academy of Sciences, 602 00 Brno, Czech RepublicDepartment of Environmental Analytical Chemistry, Institute of Analytical Chemistry, v.v.i., Czech Academy of Sciences, 602 00 Brno, Czech RepublicDepartment of Chemistry, Faculty of Science, Masaryk University, 625 00 Brno, Czech RepublicLaboratory of Molecular Morphogenesis, Institute of Animal Physiology and Genetics, v.v.i., Czech Academy of Sciences, 602 00 Brno, Czech RepublicLaboratory of Molecular Morphogenesis, Institute of Animal Physiology and Genetics, v.v.i., Czech Academy of Sciences, 602 00 Brno, Czech RepublicDepartment of Environmental Analytical Chemistry, Institute of Analytical Chemistry, v.v.i., Czech Academy of Sciences, 602 00 Brno, Czech RepublicDepartment of Environmental Analytical Chemistry, Institute of Analytical Chemistry, v.v.i., Czech Academy of Sciences, 602 00 Brno, Czech RepublicDepartment of Environmental Analytical Chemistry, Institute of Analytical Chemistry, v.v.i., Czech Academy of Sciences, 602 00 Brno, Czech RepublicDepartment of Chemistry, Faculty of Science, Masaryk University, 625 00 Brno, Czech RepublicDepartment of Environmental Analytical Chemistry, Institute of Analytical Chemistry, v.v.i., Czech Academy of Sciences, 602 00 Brno, Czech RepublicDepartment of Chemistry, Faculty of Science, Masaryk University, 625 00 Brno, Czech RepublicDepartment of Chemistry, Faculty of Science, Masaryk University, 625 00 Brno, Czech RepublicDepartment of Histology and Embryology, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech RepublicLaboratory of Molecular Morphogenesis, Institute of Animal Physiology and Genetics, v.v.i., Czech Academy of Sciences, 602 00 Brno, Czech RepublicThe inhalation of metal (including lead) nanoparticles poses a real health issue to people and animals living in polluted and/or industrial areas. In this study, we exposed mice to lead(II) nitrate nanoparticles [Pb(NO<sub>3</sub>)<sub>2</sub> NPs], which represent a highly soluble form of lead, by inhalation. We aimed to uncover the effects of their exposure on individual target organs and to reveal potential variability in the lead clearance. We examined (i) lead biodistribution in target organs using laser ablation and inductively coupled plasma mass spectrometry (LA-ICP-MS) and atomic absorption spectrometry (AAS), (ii) lead effect on histopathological changes and immune cells response in secondary target organs and (iii) the clearance ability of target organs. In the lungs and liver, Pb(NO<sub>3</sub>)<sub>2</sub> NP inhalation induced serious structural changes and their damage was present even after a 5-week clearance period despite the lead having been almost completely eliminated from the tissues. The numbers of macrophages significantly decreased after 11-week Pb(NO<sub>3</sub>)<sub>2</sub> NP inhalation; conversely, abundance of alpha-smooth muscle actin (α-SMA)-positive cells, which are responsible for augmented collagen production, increased in both tissues. Moreover, the expression of nuclear factor κB (<i>NF-κB</i>) and selected cytokines, such as tumor necrosis factor alpha (<i>TNFα</i>), transforming growth factor beta 1 (<i>TGFβ1</i>), interleukin 6(<i>IL-6</i>), <i>IL-1α</i> and <i>IL-1β</i> , displayed a tissue-specific response to lead exposure. In summary, diminished inflammatory response in tissues after Pb(NO<sub>3</sub>)<sub>2</sub> NPs inhalation was associated with prolonged negative effect of lead on tissues, as demonstrated by sustained pathological changes in target organs, even after long clearance period.https://www.mdpi.com/1422-0067/21/22/8738clearancelead nanoparticlesinhalationLA-ICP-MS imagingtoxicity |
| spellingShingle | Jana Dumková Tereza Smutná Lucie Vrlíková Bohumil Dočekal Daniela Kristeková Zbyněk Večeřa Zuzana Husáková Veronika Jakešová Adriena Jedličková Pavel Mikuška Lukáš Alexa Pavel Coufalík Michaela Tvrdoňová Kamil Křůmal Tomáš Vaculovič Viktor Kanický Aleš Hampl Marcela Buchtová A Clearance Period after Soluble Lead Nanoparticle Inhalation Did Not Ameliorate the Negative Effects on Target Tissues Due to Decreased Immune Response International Journal of Molecular Sciences clearance lead nanoparticles inhalation LA-ICP-MS imaging toxicity |
| title | A Clearance Period after Soluble Lead Nanoparticle Inhalation Did Not Ameliorate the Negative Effects on Target Tissues Due to Decreased Immune Response |
| title_full | A Clearance Period after Soluble Lead Nanoparticle Inhalation Did Not Ameliorate the Negative Effects on Target Tissues Due to Decreased Immune Response |
| title_fullStr | A Clearance Period after Soluble Lead Nanoparticle Inhalation Did Not Ameliorate the Negative Effects on Target Tissues Due to Decreased Immune Response |
| title_full_unstemmed | A Clearance Period after Soluble Lead Nanoparticle Inhalation Did Not Ameliorate the Negative Effects on Target Tissues Due to Decreased Immune Response |
| title_short | A Clearance Period after Soluble Lead Nanoparticle Inhalation Did Not Ameliorate the Negative Effects on Target Tissues Due to Decreased Immune Response |
| title_sort | clearance period after soluble lead nanoparticle inhalation did not ameliorate the negative effects on target tissues due to decreased immune response |
| topic | clearance lead nanoparticles inhalation LA-ICP-MS imaging toxicity |
| url | https://www.mdpi.com/1422-0067/21/22/8738 |
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