Effect of Bariatric Surgery on Plasma Cell-Free Mitochondrial DNA, Insulin Sensitivity and Metabolic Changes in Obese Patients
While improvement of mitochondrial function after bariatric surgery has been demonstrated, there is limited evidence about the effects of bariatric surgery on circulatory cell-free (cf) mitochondrial DNA (mtDNA) and intracellular mtDNA abundance. Plasma and peripheral blood mononuclear (PBM) cells w...
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MDPI AG
2023-09-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/11/9/2514 |
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| author | Larysa V. Yuzefovych Viktor M. Pastukh Madhuri S. Mulekar Kate Ledbetter William O. Richards Lyudmila I. Rachek |
| author_facet | Larysa V. Yuzefovych Viktor M. Pastukh Madhuri S. Mulekar Kate Ledbetter William O. Richards Lyudmila I. Rachek |
| author_sort | Larysa V. Yuzefovych |
| collection | DOAJ |
| description | While improvement of mitochondrial function after bariatric surgery has been demonstrated, there is limited evidence about the effects of bariatric surgery on circulatory cell-free (cf) mitochondrial DNA (mtDNA) and intracellular mtDNA abundance. Plasma and peripheral blood mononuclear (PBM) cells were isolated from healthy controls (HC) and bariatric surgery patients before surgery and 2 weeks, 3 months, and 6 months after surgery. At baseline, the plasma level of short cf-mtDNA (<i>ND6</i>, ~100 bp) fragments was significantly higher in obese patients compared to HC. But there was no significant variation in mean <i>ND6</i> values post-surgery. A significant positive correlation was observed between preop plasma <i>ND6</i> levels and HgbA1c, <i>ND6</i> and HOMA-IR 2 weeks post-surgery, and mtDNA content 6 months post-surgery. Interestingly, plasma from both HC and obese groups at all time points post-surgery contains long (~8 kb) cf-mtDNA fragments, suggesting the presence of near-intact and/or whole mitochondrial genomes. No significant variation was observed in mtDNA content post-surgery compared to baseline data in both PBM and skeletal muscle samples. Overall, bariatric surgery improved insulin sensitivity and other metabolic parameters without significant changes in plasma short cf-mtDNA levels or cellular mtDNA content. Our study provides novel insights about possible molecular mechanisms underlying the metabolic effects of bariatric surgery and suggests the development of new generalized approaches to characterize cf-mtDNA. |
| first_indexed | 2024-03-10T23:00:46Z |
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| id | doaj.art-d89f4872b4f34fdea77fe06447f0b1bf |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T23:00:46Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-d89f4872b4f34fdea77fe06447f0b1bf2023-11-19T09:42:24ZengMDPI AGBiomedicines2227-90592023-09-01119251410.3390/biomedicines11092514Effect of Bariatric Surgery on Plasma Cell-Free Mitochondrial DNA, Insulin Sensitivity and Metabolic Changes in Obese PatientsLarysa V. Yuzefovych0Viktor M. Pastukh1Madhuri S. Mulekar2Kate Ledbetter3William O. Richards4Lyudmila I. Rachek5Department of Pharmacology, College of Medicine, University of South Alabama, Mobile, AL 36688, USADepartment of Pharmacology, College of Medicine, University of South Alabama, Mobile, AL 36688, USADepartment of Mathematics and Statistics, College of Art and Science, University of South Alabama, Mobile, AL 36688, USADepartment of Surgery, College of Medicine, University of South Alabama, Mobile, AL 36688, USADepartment of Surgery, College of Medicine, University of South Alabama, Mobile, AL 36688, USADepartment of Pharmacology, College of Medicine, University of South Alabama, Mobile, AL 36688, USAWhile improvement of mitochondrial function after bariatric surgery has been demonstrated, there is limited evidence about the effects of bariatric surgery on circulatory cell-free (cf) mitochondrial DNA (mtDNA) and intracellular mtDNA abundance. Plasma and peripheral blood mononuclear (PBM) cells were isolated from healthy controls (HC) and bariatric surgery patients before surgery and 2 weeks, 3 months, and 6 months after surgery. At baseline, the plasma level of short cf-mtDNA (<i>ND6</i>, ~100 bp) fragments was significantly higher in obese patients compared to HC. But there was no significant variation in mean <i>ND6</i> values post-surgery. A significant positive correlation was observed between preop plasma <i>ND6</i> levels and HgbA1c, <i>ND6</i> and HOMA-IR 2 weeks post-surgery, and mtDNA content 6 months post-surgery. Interestingly, plasma from both HC and obese groups at all time points post-surgery contains long (~8 kb) cf-mtDNA fragments, suggesting the presence of near-intact and/or whole mitochondrial genomes. No significant variation was observed in mtDNA content post-surgery compared to baseline data in both PBM and skeletal muscle samples. Overall, bariatric surgery improved insulin sensitivity and other metabolic parameters without significant changes in plasma short cf-mtDNA levels or cellular mtDNA content. Our study provides novel insights about possible molecular mechanisms underlying the metabolic effects of bariatric surgery and suggests the development of new generalized approaches to characterize cf-mtDNA.https://www.mdpi.com/2227-9059/11/9/2514circulating cell-free mitochondrial DNAobesityinsulin resistancetype 2 diabetes |
| spellingShingle | Larysa V. Yuzefovych Viktor M. Pastukh Madhuri S. Mulekar Kate Ledbetter William O. Richards Lyudmila I. Rachek Effect of Bariatric Surgery on Plasma Cell-Free Mitochondrial DNA, Insulin Sensitivity and Metabolic Changes in Obese Patients Biomedicines circulating cell-free mitochondrial DNA obesity insulin resistance type 2 diabetes |
| title | Effect of Bariatric Surgery on Plasma Cell-Free Mitochondrial DNA, Insulin Sensitivity and Metabolic Changes in Obese Patients |
| title_full | Effect of Bariatric Surgery on Plasma Cell-Free Mitochondrial DNA, Insulin Sensitivity and Metabolic Changes in Obese Patients |
| title_fullStr | Effect of Bariatric Surgery on Plasma Cell-Free Mitochondrial DNA, Insulin Sensitivity and Metabolic Changes in Obese Patients |
| title_full_unstemmed | Effect of Bariatric Surgery on Plasma Cell-Free Mitochondrial DNA, Insulin Sensitivity and Metabolic Changes in Obese Patients |
| title_short | Effect of Bariatric Surgery on Plasma Cell-Free Mitochondrial DNA, Insulin Sensitivity and Metabolic Changes in Obese Patients |
| title_sort | effect of bariatric surgery on plasma cell free mitochondrial dna insulin sensitivity and metabolic changes in obese patients |
| topic | circulating cell-free mitochondrial DNA obesity insulin resistance type 2 diabetes |
| url | https://www.mdpi.com/2227-9059/11/9/2514 |
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