Fatty Acid Profile of Mature Red Blood Cell Membranes and Dietary Intake as a New Approach to Characterize Children with Overweight and Obesity
Obesity is a chronic metabolic disease of high complexity and of multifactorial origin. Understanding the effects of nutrition on childhood obesity metabolism remains a challenge. The aim of this study was to determine the fatty acid (FA) profile of red blood cell (RBC) membranes as a comprehensive...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-11-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/12/11/3446 |
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| author | Iker Jauregibeitia Kevin Portune Itxaso Rica Itziar Tueros Olaia Velasco Gema Grau Nerea Trebolazabala Luis Castaño Anna Vita Larocca Carla Ferreri Sara Arranz |
| author_facet | Iker Jauregibeitia Kevin Portune Itxaso Rica Itziar Tueros Olaia Velasco Gema Grau Nerea Trebolazabala Luis Castaño Anna Vita Larocca Carla Ferreri Sara Arranz |
| author_sort | Iker Jauregibeitia |
| collection | DOAJ |
| description | Obesity is a chronic metabolic disease of high complexity and of multifactorial origin. Understanding the effects of nutrition on childhood obesity metabolism remains a challenge. The aim of this study was to determine the fatty acid (FA) profile of red blood cell (RBC) membranes as a comprehensive biomarker of children’s obesity metabolism, together with the evaluation of their dietary intake. An observational study was carried out on 209 children (107 healthy controls, 41 who were overweight and 61 with obesity) between 6 and 16 years of age. Mature RBC membrane phospholipids were analyzed for FA composition by gas chromatography-mass spectrometry (GC-MS). Dietary habits were evaluated using validated food frequency questionnaires (FFQ) and the Mediterranean Diet Quality Index for children (KIDMED) test. Compared to children with normal weight, children with obesity showed an inflammatory profile in mature RBC FAs, evidenced by higher levels of ω-6 polyunsaturated FAs (mainly arachidonic acid, <i>p</i> < 0.001). Children who were overweight or obese presented lower levels of monounsaturated FA (MUFA) compared to children with normal weight (<i>p</i> = 0.001 and <i>p</i> = 0.03, respectively), resulting in an increased saturated fatty acid (SFA)/MUFA ratio. A lower intake of nuts was observed for children with obesity. A comprehensive membrane lipidomic profile approach in children with obesity will contribute to a better understanding of the metabolic differences present in these individuals. |
| first_indexed | 2024-03-10T14:58:19Z |
| format | Article |
| id | doaj.art-d8a2051d4b624f7a9351a447d19fd506 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T14:58:19Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-d8a2051d4b624f7a9351a447d19fd5062023-11-20T20:28:23ZengMDPI AGNutrients2072-66432020-11-011211344610.3390/nu12113446Fatty Acid Profile of Mature Red Blood Cell Membranes and Dietary Intake as a New Approach to Characterize Children with Overweight and ObesityIker Jauregibeitia0Kevin Portune1Itxaso Rica2Itziar Tueros3Olaia Velasco4Gema Grau5Nerea Trebolazabala6Luis Castaño7Anna Vita Larocca8Carla Ferreri9Sara Arranz10AZTI, Food Research, Basque Research and Technology Alliance (BRTA), Parque Tecnológico de Bizkaia, Astondo Bidea, Edificio 609, 48160 Derio, SpainAZTI, Food Research, Basque Research and Technology Alliance (BRTA), Parque Tecnológico de Bizkaia, Astondo Bidea, Edificio 609, 48160 Derio, SpainBiocruces Bizkaia Health Research Institute, Cruces University Hospital, CIBERDEM/CIBERER, UPV/EHU, 48903 Barakaldo, SpainAZTI, Food Research, Basque Research and Technology Alliance (BRTA), Parque Tecnológico de Bizkaia, Astondo Bidea, Edificio 609, 48160 Derio, SpainBiocruces Bizkaia Health Research Institute, Cruces University Hospital, CIBERDEM/CIBERER, UPV/EHU, 48903 Barakaldo, SpainBiocruces Bizkaia Health Research Institute, Cruces University Hospital, CIBERDEM/CIBERER, UPV/EHU, 48903 Barakaldo, SpainBiocruces Bizkaia Health Research Institute, Cruces University Hospital, CIBERDEM/CIBERER, UPV/EHU, 48903 Barakaldo, SpainBiocruces Bizkaia Health Research Institute, Cruces University Hospital, CIBERDEM/CIBERER, UPV/EHU, 48903 Barakaldo, SpainLipidomic Laboratory, Lipinutragen srl, Via di Corticella 181/4, 40128 Bologna, ItalyConsiglio Nazionale delle Ricerche, ISOF, Via Piero Gobetti 101, 40129 Bologna, ItalyAZTI, Food Research, Basque Research and Technology Alliance (BRTA), Parque Tecnológico de Bizkaia, Astondo Bidea, Edificio 609, 48160 Derio, SpainObesity is a chronic metabolic disease of high complexity and of multifactorial origin. Understanding the effects of nutrition on childhood obesity metabolism remains a challenge. The aim of this study was to determine the fatty acid (FA) profile of red blood cell (RBC) membranes as a comprehensive biomarker of children’s obesity metabolism, together with the evaluation of their dietary intake. An observational study was carried out on 209 children (107 healthy controls, 41 who were overweight and 61 with obesity) between 6 and 16 years of age. Mature RBC membrane phospholipids were analyzed for FA composition by gas chromatography-mass spectrometry (GC-MS). Dietary habits were evaluated using validated food frequency questionnaires (FFQ) and the Mediterranean Diet Quality Index for children (KIDMED) test. Compared to children with normal weight, children with obesity showed an inflammatory profile in mature RBC FAs, evidenced by higher levels of ω-6 polyunsaturated FAs (mainly arachidonic acid, <i>p</i> < 0.001). Children who were overweight or obese presented lower levels of monounsaturated FA (MUFA) compared to children with normal weight (<i>p</i> = 0.001 and <i>p</i> = 0.03, respectively), resulting in an increased saturated fatty acid (SFA)/MUFA ratio. A lower intake of nuts was observed for children with obesity. A comprehensive membrane lipidomic profile approach in children with obesity will contribute to a better understanding of the metabolic differences present in these individuals.https://www.mdpi.com/2072-6643/12/11/3446childhood obesityinflammationmembrane lipidomeomega-6 fatty acidsred blood cell |
| spellingShingle | Iker Jauregibeitia Kevin Portune Itxaso Rica Itziar Tueros Olaia Velasco Gema Grau Nerea Trebolazabala Luis Castaño Anna Vita Larocca Carla Ferreri Sara Arranz Fatty Acid Profile of Mature Red Blood Cell Membranes and Dietary Intake as a New Approach to Characterize Children with Overweight and Obesity Nutrients childhood obesity inflammation membrane lipidome omega-6 fatty acids red blood cell |
| title | Fatty Acid Profile of Mature Red Blood Cell Membranes and Dietary Intake as a New Approach to Characterize Children with Overweight and Obesity |
| title_full | Fatty Acid Profile of Mature Red Blood Cell Membranes and Dietary Intake as a New Approach to Characterize Children with Overweight and Obesity |
| title_fullStr | Fatty Acid Profile of Mature Red Blood Cell Membranes and Dietary Intake as a New Approach to Characterize Children with Overweight and Obesity |
| title_full_unstemmed | Fatty Acid Profile of Mature Red Blood Cell Membranes and Dietary Intake as a New Approach to Characterize Children with Overweight and Obesity |
| title_short | Fatty Acid Profile of Mature Red Blood Cell Membranes and Dietary Intake as a New Approach to Characterize Children with Overweight and Obesity |
| title_sort | fatty acid profile of mature red blood cell membranes and dietary intake as a new approach to characterize children with overweight and obesity |
| topic | childhood obesity inflammation membrane lipidome omega-6 fatty acids red blood cell |
| url | https://www.mdpi.com/2072-6643/12/11/3446 |
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