Response of bacterial community metabolites to bacterial wilt caused by Ralstonia solanacearum: a multi-omics analysis
The soil microbial community plays a critical role in promoting robust plant growth and serves as an effective defence mechanism against root pathogens. Current research has focused on unravelling the compositions and functions of diverse microbial taxa in plant rhizospheres invaded by Ralstonia sol...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1339478/full |
| _version_ | 1797349464826970112 |
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| author | Chengjian Wei Chengjian Wei Jinchang Liang Rui Wang Luping Chi Wenjing Wang Jun Tan Heli Shi Xueru Song Zhenzhen Cui Qiang Xie Dejie Cheng Xiaoqiang Wang |
| author_facet | Chengjian Wei Chengjian Wei Jinchang Liang Rui Wang Luping Chi Wenjing Wang Jun Tan Heli Shi Xueru Song Zhenzhen Cui Qiang Xie Dejie Cheng Xiaoqiang Wang |
| author_sort | Chengjian Wei |
| collection | DOAJ |
| description | The soil microbial community plays a critical role in promoting robust plant growth and serves as an effective defence mechanism against root pathogens. Current research has focused on unravelling the compositions and functions of diverse microbial taxa in plant rhizospheres invaded by Ralstonia solanacearum, however, the specific mechanisms by which key microbial groups with distinct functions exert their effects remain unclear. In this study, we employed a combination of amplicon sequencing and metabolomics analysis to investigate the principal metabolic mechanisms of key microbial taxa in plant rhizosphere soil. Compared to the healthy tobacco rhizosphere samples, the bacterial diversity and co-occurrence network of the diseased tobacco rhizosphere soil were significantly reduced. Notably, certain genera, including Gaiella, Rhodoplanes, and MND1 (Nitrosomonadaceae), were found to be significantly more abundant in the rhizosphere of healthy plants than in that of diseased plants. Eight environmental factors, including exchangeable magnesium, available phosphorus, and pH, were found to be crucial factors influencing the composition of the microbial community. Ralstonia displayed negative correlations with pH, exchangeable magnesium, and cation exchange flux, but showed a positive correlation with available iron. Furthermore, metabolomic analysis revealed that the metabolic pathways related to the synthesis of various antibacterial compounds were significantly enriched in the healthy group. The correlation analysis results indicate that the bacterial genera Polycyclovorans, Lysobacter, Pseudomonas, and Nitrosospira may participate in the synthesis of antibacterial compounds. Collectively, our findings contribute to a more in-depth understanding of disease resistance mechanisms within healthy microbial communities and provide a theoretical foundation for the development of targeted strategies using beneficial microorganisms to suppress disease occurrence. |
| first_indexed | 2024-03-08T12:30:38Z |
| format | Article |
| id | doaj.art-d8a46197f107431c8e0462102e8ddc67 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-08T12:30:38Z |
| publishDate | 2024-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-d8a46197f107431c8e0462102e8ddc672024-01-22T04:18:43ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2024-01-011410.3389/fpls.2023.13394781339478Response of bacterial community metabolites to bacterial wilt caused by Ralstonia solanacearum: a multi-omics analysisChengjian Wei0Chengjian Wei1Jinchang Liang2Rui Wang3Luping Chi4Wenjing Wang5Jun Tan6Heli Shi7Xueru Song8Zhenzhen Cui9Qiang Xie10Dejie Cheng11Xiaoqiang Wang12College of Agriculture, Guangxi University, Nanning, ChinaKey Laboratory of Tobacco Pest Monitoring & Integrated Management, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, ChinaKey Laboratory of Tobacco Pest Monitoring & Integrated Management, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, ChinaEnshi Tobacco Science and Technology Center, Enshi, ChinaKey Laboratory of Tobacco Pest Monitoring & Integrated Management, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, ChinaKey Laboratory of Tobacco Pest Monitoring & Integrated Management, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, ChinaEnshi Tobacco Science and Technology Center, Enshi, ChinaEnshi Tobacco Science and Technology Center, Enshi, ChinaEngineering Center for Biological Control of Diseases and Pests in Tobacco Industry, Yuxi, ChinaKey Laboratory of Tobacco Pest Monitoring & Integrated Management, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, ChinaSichuan Tobacco Science and Technology Center, Chengdu, ChinaCollege of Agriculture, Guangxi University, Nanning, ChinaKey Laboratory of Tobacco Pest Monitoring & Integrated Management, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, ChinaThe soil microbial community plays a critical role in promoting robust plant growth and serves as an effective defence mechanism against root pathogens. Current research has focused on unravelling the compositions and functions of diverse microbial taxa in plant rhizospheres invaded by Ralstonia solanacearum, however, the specific mechanisms by which key microbial groups with distinct functions exert their effects remain unclear. In this study, we employed a combination of amplicon sequencing and metabolomics analysis to investigate the principal metabolic mechanisms of key microbial taxa in plant rhizosphere soil. Compared to the healthy tobacco rhizosphere samples, the bacterial diversity and co-occurrence network of the diseased tobacco rhizosphere soil were significantly reduced. Notably, certain genera, including Gaiella, Rhodoplanes, and MND1 (Nitrosomonadaceae), were found to be significantly more abundant in the rhizosphere of healthy plants than in that of diseased plants. Eight environmental factors, including exchangeable magnesium, available phosphorus, and pH, were found to be crucial factors influencing the composition of the microbial community. Ralstonia displayed negative correlations with pH, exchangeable magnesium, and cation exchange flux, but showed a positive correlation with available iron. Furthermore, metabolomic analysis revealed that the metabolic pathways related to the synthesis of various antibacterial compounds were significantly enriched in the healthy group. The correlation analysis results indicate that the bacterial genera Polycyclovorans, Lysobacter, Pseudomonas, and Nitrosospira may participate in the synthesis of antibacterial compounds. Collectively, our findings contribute to a more in-depth understanding of disease resistance mechanisms within healthy microbial communities and provide a theoretical foundation for the development of targeted strategies using beneficial microorganisms to suppress disease occurrence.https://www.frontiersin.org/articles/10.3389/fpls.2023.1339478/fullkeystone taxametabolitesbacterial wiltrhizospheremicrobiomes |
| spellingShingle | Chengjian Wei Chengjian Wei Jinchang Liang Rui Wang Luping Chi Wenjing Wang Jun Tan Heli Shi Xueru Song Zhenzhen Cui Qiang Xie Dejie Cheng Xiaoqiang Wang Response of bacterial community metabolites to bacterial wilt caused by Ralstonia solanacearum: a multi-omics analysis Frontiers in Plant Science keystone taxa metabolites bacterial wilt rhizosphere microbiomes |
| title | Response of bacterial community metabolites to bacterial wilt caused by Ralstonia solanacearum: a multi-omics analysis |
| title_full | Response of bacterial community metabolites to bacterial wilt caused by Ralstonia solanacearum: a multi-omics analysis |
| title_fullStr | Response of bacterial community metabolites to bacterial wilt caused by Ralstonia solanacearum: a multi-omics analysis |
| title_full_unstemmed | Response of bacterial community metabolites to bacterial wilt caused by Ralstonia solanacearum: a multi-omics analysis |
| title_short | Response of bacterial community metabolites to bacterial wilt caused by Ralstonia solanacearum: a multi-omics analysis |
| title_sort | response of bacterial community metabolites to bacterial wilt caused by ralstonia solanacearum a multi omics analysis |
| topic | keystone taxa metabolites bacterial wilt rhizosphere microbiomes |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1339478/full |
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