A pan-cancer analysis of the oncogenic role of ribonucleotide reductase subunit M2 in human tumors
Background Recent studies have identified ribonucleotide reductase subunit M2 (RRM2) as a putative promoter of tumors. However, no systematic analysis of its carcinogenicity has been conducted. Methods The potential functions of RRM2 in various tumor types were investigated using data from the Genot...
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PeerJ Inc.
2022-11-01
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| author | Yaqun Li Wenhuan Fu Zikai Geng Yun Song Xionggang Yang Tianye He Jian Wu Bin Wang |
| author_facet | Yaqun Li Wenhuan Fu Zikai Geng Yun Song Xionggang Yang Tianye He Jian Wu Bin Wang |
| author_sort | Yaqun Li |
| collection | DOAJ |
| description | Background Recent studies have identified ribonucleotide reductase subunit M2 (RRM2) as a putative promoter of tumors. However, no systematic analysis of its carcinogenicity has been conducted. Methods The potential functions of RRM2 in various tumor types were investigated using data from the Genotype-Tissue Expression (GTEx), the Clinical Proteomic Tumor Analysis Consortium (CPTAC), the Cancer Genome Atlas (TCGA), the Human Protein Atlas (HPA), cBioPortal, GEPIA, String, and Gene Set Enrichment Analysis (GSEA). We analyzed the difference in mRNA and protein expression, pathological stage, survival, mutation, tumor microenvironment (TME), and immune cell infiltration in relation to RRM2. Meanwhile, using TCGA and the Tumor Immune Estimation Resource 2 (TIMER 2), the associations between RRM2 expression, immune infiltration, and immune-related genes were assessed. Additionally, CCK-8, Edu and RT-PCR assays were used to validate that RRM2 acts as an oncogene in liver cancer cells and its association with HBx. A cohort of liver hepatocellular carcinoma (LIHC) patients (n=154) from Huashan Hospital was analyzed for the expression of RRM2 and the association between RRM2 and immune infiltration. Results Using the GTEx and TCGA databases, we discovered that 28 tumors expressed RRM2 at significantly higher levels than the corresponding normal tissues. Increased RRM2 expression may be predictive of a poor overall survival (OS) in patients with seven different cancers. GO, KEGG, and GSEA analyses revealed that the biological process of RRM2 was associated with the regulation of carcinogenic processes and immune pathways in a variety of tumor types. The expression of RRM2 was highly correlated with maker genes involved in immune activation and immunosuppression, immune checkpoints, DNA mismatch repair system (MMR), and the infiltration levels of Tregs and macrophages (TAMs), suggesting that the carcinogenic effect of RRM2 may be achieved by regulating immune related genes. Moreover, as demonstrated by CCK-8 and Edu assays, RRM2 was an oncogene in liver cancer cells. We confirmed for the first time that RRM2 was significantly upregulated by HBx, suggesting that RRM2 may be a key regulator of LIHC induced by HBV. IHC analysis validated the upregulated expression of RRM2 protein and its correlation with immune infiltration makers in a LIHC patient cohort. Conclusion RRM2 may be a valuable molecular biomarker for predicting prognosis and immunotherapeutic efficacy in pan-cancer, particularly in LIHC. |
| first_indexed | 2024-03-09T08:18:58Z |
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| language | English |
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| spelling | doaj.art-d8a5badc74b04dec95c6b4cbd32dcbb12023-12-02T21:55:26ZengPeerJ Inc.PeerJ2167-83592022-11-0110e1443210.7717/peerj.14432A pan-cancer analysis of the oncogenic role of ribonucleotide reductase subunit M2 in human tumorsYaqun Li0Wenhuan Fu1Zikai Geng2Yun Song3Xionggang Yang4Tianye He5Jian Wu6Bin Wang7Department of Pharmacy, Huashan Hospital, Fudan University, Shanghai, ChinaDepartment of Pharmacy, Huashan Hospital, Fudan University, Shanghai, ChinaPharmacy School, Binzhou Medical University, Shandong, ChinaDepartment of Pharmacy, Huashan Hospital, Fudan University, Shanghai, ChinaDepartment of Orthopedic Surgery, Huashan Hospital, Fudan University, Shanghai, ChinaDepartment of Pharmacy, Huashan Hospital, Fudan University, Shanghai, ChinaDepartment of Pharmacy, Huashan Hospital, Fudan University, Shanghai, ChinaDepartment of Pharmacy, Huashan Hospital, Fudan University, Shanghai, ChinaBackground Recent studies have identified ribonucleotide reductase subunit M2 (RRM2) as a putative promoter of tumors. However, no systematic analysis of its carcinogenicity has been conducted. Methods The potential functions of RRM2 in various tumor types were investigated using data from the Genotype-Tissue Expression (GTEx), the Clinical Proteomic Tumor Analysis Consortium (CPTAC), the Cancer Genome Atlas (TCGA), the Human Protein Atlas (HPA), cBioPortal, GEPIA, String, and Gene Set Enrichment Analysis (GSEA). We analyzed the difference in mRNA and protein expression, pathological stage, survival, mutation, tumor microenvironment (TME), and immune cell infiltration in relation to RRM2. Meanwhile, using TCGA and the Tumor Immune Estimation Resource 2 (TIMER 2), the associations between RRM2 expression, immune infiltration, and immune-related genes were assessed. Additionally, CCK-8, Edu and RT-PCR assays were used to validate that RRM2 acts as an oncogene in liver cancer cells and its association with HBx. A cohort of liver hepatocellular carcinoma (LIHC) patients (n=154) from Huashan Hospital was analyzed for the expression of RRM2 and the association between RRM2 and immune infiltration. Results Using the GTEx and TCGA databases, we discovered that 28 tumors expressed RRM2 at significantly higher levels than the corresponding normal tissues. Increased RRM2 expression may be predictive of a poor overall survival (OS) in patients with seven different cancers. GO, KEGG, and GSEA analyses revealed that the biological process of RRM2 was associated with the regulation of carcinogenic processes and immune pathways in a variety of tumor types. The expression of RRM2 was highly correlated with maker genes involved in immune activation and immunosuppression, immune checkpoints, DNA mismatch repair system (MMR), and the infiltration levels of Tregs and macrophages (TAMs), suggesting that the carcinogenic effect of RRM2 may be achieved by regulating immune related genes. Moreover, as demonstrated by CCK-8 and Edu assays, RRM2 was an oncogene in liver cancer cells. We confirmed for the first time that RRM2 was significantly upregulated by HBx, suggesting that RRM2 may be a key regulator of LIHC induced by HBV. IHC analysis validated the upregulated expression of RRM2 protein and its correlation with immune infiltration makers in a LIHC patient cohort. Conclusion RRM2 may be a valuable molecular biomarker for predicting prognosis and immunotherapeutic efficacy in pan-cancer, particularly in LIHC.https://peerj.com/articles/14432.pdfRRM2Pan-cancerImmune infiltrationPrognosisTumor microenvironmentHepatitis B |
| spellingShingle | Yaqun Li Wenhuan Fu Zikai Geng Yun Song Xionggang Yang Tianye He Jian Wu Bin Wang A pan-cancer analysis of the oncogenic role of ribonucleotide reductase subunit M2 in human tumors PeerJ RRM2 Pan-cancer Immune infiltration Prognosis Tumor microenvironment Hepatitis B |
| title | A pan-cancer analysis of the oncogenic role of ribonucleotide reductase subunit M2 in human tumors |
| title_full | A pan-cancer analysis of the oncogenic role of ribonucleotide reductase subunit M2 in human tumors |
| title_fullStr | A pan-cancer analysis of the oncogenic role of ribonucleotide reductase subunit M2 in human tumors |
| title_full_unstemmed | A pan-cancer analysis of the oncogenic role of ribonucleotide reductase subunit M2 in human tumors |
| title_short | A pan-cancer analysis of the oncogenic role of ribonucleotide reductase subunit M2 in human tumors |
| title_sort | pan cancer analysis of the oncogenic role of ribonucleotide reductase subunit m2 in human tumors |
| topic | RRM2 Pan-cancer Immune infiltration Prognosis Tumor microenvironment Hepatitis B |
| url | https://peerj.com/articles/14432.pdf |
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