Lycium barbarum polysaccharide alleviates DSS-induced chronic ulcerative colitis by restoring intestinal barrier function and modulating gut microbiota
AbstractPurpose This study examined the protective effects and mechanism of Lycium barbarum polysaccharides (LBP) in the context of intestinal barrier function and intestinal microbiota in mice with dextran sulfate sodium (DSS)-induced chronic ulcerative colitis (UC).Methods C57BL/6J male mice were...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Annals of Medicine |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/07853890.2023.2290213 |
| _version_ | 1797302173163323392 |
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| author | Zhi-Yu Li Lan-Hui Lin He-Jun Liang Ya-Qi Li Fu-Qian Zhao Ting-Yi Sun Zi-Yu Liu Jing-Yi Zhu Feng Gu Jia-Ning Xu Qi-Yuan Hao De-Shan Zhou Hui-Hong Zhai |
| author_facet | Zhi-Yu Li Lan-Hui Lin He-Jun Liang Ya-Qi Li Fu-Qian Zhao Ting-Yi Sun Zi-Yu Liu Jing-Yi Zhu Feng Gu Jia-Ning Xu Qi-Yuan Hao De-Shan Zhou Hui-Hong Zhai |
| author_sort | Zhi-Yu Li |
| collection | DOAJ |
| description | AbstractPurpose This study examined the protective effects and mechanism of Lycium barbarum polysaccharides (LBP) in the context of intestinal barrier function and intestinal microbiota in mice with dextran sulfate sodium (DSS)-induced chronic ulcerative colitis (UC).Methods C57BL/6J male mice were assigned to a standard normal diet without DSS (control group), a normal diet with DSS (DSS group, 2% DSS given discontinuously for 3 weeks) or a normal diet supplemented with LBP (1% dry feed weight, LBP group, 2% DSS given discontinuously for 3 weeks) for a total of 8 weeks, at which point colonic tissues and caecal contents were collected.Results LBP exerted a significant effect against colitis by increasing body weight, colon length, DAI and histopathological scores. LBP inhibited proinflammatory cytokines (IL-1β, IL-6, iNOS and TNF-α) expression, improved anti-inflammatory cytokine (IL-10) expression, promoted the expression of tight junction proteins (Occludin and ZO-1) via nuclear factor erythroid 2-related factor 2 (Nrf2) activation and decreased Claudin-2 expression to maintain the intestinal mucosal barrier. In addition, the abundances of some probiotics (Ruminococcaceae, Lactobacillus, Butyricicoccus, and Akkermansia) were decreased with DSS treatment but increased obviously with LBP treatment. And LBP reduced the abundance of conditional pathogens associated with UC (Mucispirillum and Sutterella). Furthermore, LBP improved the production of short-chain fatty acids (SCFAs), including acetic acid, propionic acid, butyric acid and isobutyric acid.Conclusion LBP can alleviate DSS-induced UC by regulating inflammatory cytokines and tight junction proteins. Moreover, LBP promotes probiotics, suppresses conditional pathogens and increases SCFAs production, showing a strong prebiotic effect. |
| first_indexed | 2024-03-07T23:33:00Z |
| format | Article |
| id | doaj.art-d8b0945773ba4be29c4bc7e0053d65ce |
| institution | Directory Open Access Journal |
| issn | 0785-3890 1365-2060 |
| language | English |
| last_indexed | 2024-03-07T23:33:00Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Annals of Medicine |
| spelling | doaj.art-d8b0945773ba4be29c4bc7e0053d65ce2024-02-20T11:58:24ZengTaylor & Francis GroupAnnals of Medicine0785-38901365-20602023-12-0155210.1080/07853890.2023.2290213Lycium barbarum polysaccharide alleviates DSS-induced chronic ulcerative colitis by restoring intestinal barrier function and modulating gut microbiotaZhi-Yu Li0Lan-Hui Lin1He-Jun Liang2Ya-Qi Li3Fu-Qian Zhao4Ting-Yi Sun5Zi-Yu Liu6Jing-Yi Zhu7Feng Gu8Jia-Ning Xu9Qi-Yuan Hao10De-Shan Zhou11Hui-Hong Zhai12Department of Gastroenterology, Beijing Friendship Hospital Affiliated to Capital Medical University, Beijing, ChinaNational Clinical Research Center for Digestive Diseases, Beijing, ChinaNational Clinical Research Center for Digestive Diseases, Beijing, ChinaDepartment of Histology and Embryology, School of Basic Medical Sciences, Capital Medical University, Beijing, ChinaDepartment of Histology and Embryology, School of Basic Medical Sciences, Capital Medical University, Beijing, ChinaDepartment of Histology and Embryology, School of Basic Medical Sciences, Capital Medical University, Beijing, ChinaNational Clinical Research Center for Digestive Diseases, Beijing, ChinaNational Clinical Research Center for Digestive Diseases, Beijing, ChinaNational Clinical Research Center for Digestive Diseases, Beijing, ChinaNational Clinical Research Center for Digestive Diseases, Beijing, ChinaNational Clinical Research Center for Digestive Diseases, Beijing, ChinaDepartment of Histology and Embryology, School of Basic Medical Sciences, Capital Medical University, Beijing, ChinaNational Clinical Research Center for Digestive Diseases, Beijing, ChinaAbstractPurpose This study examined the protective effects and mechanism of Lycium barbarum polysaccharides (LBP) in the context of intestinal barrier function and intestinal microbiota in mice with dextran sulfate sodium (DSS)-induced chronic ulcerative colitis (UC).Methods C57BL/6J male mice were assigned to a standard normal diet without DSS (control group), a normal diet with DSS (DSS group, 2% DSS given discontinuously for 3 weeks) or a normal diet supplemented with LBP (1% dry feed weight, LBP group, 2% DSS given discontinuously for 3 weeks) for a total of 8 weeks, at which point colonic tissues and caecal contents were collected.Results LBP exerted a significant effect against colitis by increasing body weight, colon length, DAI and histopathological scores. LBP inhibited proinflammatory cytokines (IL-1β, IL-6, iNOS and TNF-α) expression, improved anti-inflammatory cytokine (IL-10) expression, promoted the expression of tight junction proteins (Occludin and ZO-1) via nuclear factor erythroid 2-related factor 2 (Nrf2) activation and decreased Claudin-2 expression to maintain the intestinal mucosal barrier. In addition, the abundances of some probiotics (Ruminococcaceae, Lactobacillus, Butyricicoccus, and Akkermansia) were decreased with DSS treatment but increased obviously with LBP treatment. And LBP reduced the abundance of conditional pathogens associated with UC (Mucispirillum and Sutterella). Furthermore, LBP improved the production of short-chain fatty acids (SCFAs), including acetic acid, propionic acid, butyric acid and isobutyric acid.Conclusion LBP can alleviate DSS-induced UC by regulating inflammatory cytokines and tight junction proteins. Moreover, LBP promotes probiotics, suppresses conditional pathogens and increases SCFAs production, showing a strong prebiotic effect.https://www.tandfonline.com/doi/10.1080/07853890.2023.2290213Lycium barbarum polysaccharidesulcerative colitistight junction proteinsgut microbiota |
| spellingShingle | Zhi-Yu Li Lan-Hui Lin He-Jun Liang Ya-Qi Li Fu-Qian Zhao Ting-Yi Sun Zi-Yu Liu Jing-Yi Zhu Feng Gu Jia-Ning Xu Qi-Yuan Hao De-Shan Zhou Hui-Hong Zhai Lycium barbarum polysaccharide alleviates DSS-induced chronic ulcerative colitis by restoring intestinal barrier function and modulating gut microbiota Annals of Medicine Lycium barbarum polysaccharides ulcerative colitis tight junction proteins gut microbiota |
| title | Lycium barbarum polysaccharide alleviates DSS-induced chronic ulcerative colitis by restoring intestinal barrier function and modulating gut microbiota |
| title_full | Lycium barbarum polysaccharide alleviates DSS-induced chronic ulcerative colitis by restoring intestinal barrier function and modulating gut microbiota |
| title_fullStr | Lycium barbarum polysaccharide alleviates DSS-induced chronic ulcerative colitis by restoring intestinal barrier function and modulating gut microbiota |
| title_full_unstemmed | Lycium barbarum polysaccharide alleviates DSS-induced chronic ulcerative colitis by restoring intestinal barrier function and modulating gut microbiota |
| title_short | Lycium barbarum polysaccharide alleviates DSS-induced chronic ulcerative colitis by restoring intestinal barrier function and modulating gut microbiota |
| title_sort | lycium barbarum polysaccharide alleviates dss induced chronic ulcerative colitis by restoring intestinal barrier function and modulating gut microbiota |
| topic | Lycium barbarum polysaccharides ulcerative colitis tight junction proteins gut microbiota |
| url | https://www.tandfonline.com/doi/10.1080/07853890.2023.2290213 |
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