Characterization of Novel Integrons, In1085 and In1086, and the Surrounding Genes in Plasmids from Enterobacteriaceae, and the Role for attCaadA16 Structural Features during attI1 × attC Integration
Novel class 1 integrons In1085 and In1086, containing the class D β-lactamase -encoding gene blaOXA, were identified in clinical enterobacterial strains. In this study, we aimed to characterize the genetic contexts of In1085 and In1086, with the goal of identifying putative mechanisms of integron mo...
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| Language: | English |
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Frontiers Media S.A.
2017-06-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2017.01003/full |
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| author | Dongguo Wang Wei Hou Jiayu Chen Linjun Yang Zhihui Liu Zhe Yin Jiao Feng Dongsheng Zhou |
| author_facet | Dongguo Wang Wei Hou Jiayu Chen Linjun Yang Zhihui Liu Zhe Yin Jiao Feng Dongsheng Zhou |
| author_sort | Dongguo Wang |
| collection | DOAJ |
| description | Novel class 1 integrons In1085 and In1086, containing the class D β-lactamase -encoding gene blaOXA, were identified in clinical enterobacterial strains. In this study, we aimed to characterize the genetic contexts of In1085 and In1086, with the goal of identifying putative mechanisms of integron mobilization. Four plasmids, approximately 5.3, 5.3, 5.7, and 6.6 kb, from 71 clinical Enterobacteriaceae strains were found to contain class 1 integrons (In37, In62, In1085, and In1086, respectively). Two of these plasmids, pEco336 and pNsa292, containing In1085 and In1086, respectively, were further characterized by antibiotic susceptibility testing, conjugation experiments, PCR, sequencing, and gene mapping. The OXA-type carbapenemase activities of the parental strains were also assessed. The results revealed that the novel integrons had different genetic environments, and therefore demonstrated diverse biochemical characteristics. Using evolutionary inferences based on the recombination of gene cassettes, we also identified a role for attCaadA16 structural features during attI1 × attC insertion reactions. Our analysis showed that gene cassette insertions in the bottom strand of attCaadA16 in the correct orientation lead to the expression the encoded genes from the Pc promoter. Our study suggests that the genetic features harbored within the integrons are inserted in a discernable pattern, involving the stepwise and parallel evolution of class 1 integron variations under antibiotic selection pressures in a clinical setting. |
| first_indexed | 2024-12-11T01:49:52Z |
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| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-11T01:49:52Z |
| publishDate | 2017-06-01 |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-d8b2c5fe50604de983c86e7418cb89952022-12-22T01:24:47ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-06-01810.3389/fmicb.2017.01003264719Characterization of Novel Integrons, In1085 and In1086, and the Surrounding Genes in Plasmids from Enterobacteriaceae, and the Role for attCaadA16 Structural Features during attI1 × attC IntegrationDongguo Wang0Wei Hou1Jiayu Chen2Linjun Yang3Zhihui Liu4Zhe Yin5Jiao Feng6Dongsheng Zhou7Department of Clinical Laboratory Medicine, Taizhou Municipal Hospital Affiliated with Taizhou University and the Institute of Molecular Diagnostics of Taizhou UniversityTaizhou, ChinaDepartment of Infection, Taizhou Municipal Hospital Affiliated with Taizhou UniversityTaizhou, ChinaBasic Department, Shaoxing UniversityTaizhou, ChinaDepartment of the Thyroid Gland and Breast Surgery, Taizhou Municipal Hospital Affiliated with Taizhou UniversityTaizhou, ChinaDepartment of Stomatology, Taizhou Municipal Hospital Affiliated with the Medical College of Taizhou UniversityTaizhou, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and EpidemiologyBeijing, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and EpidemiologyBeijing, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and EpidemiologyBeijing, ChinaNovel class 1 integrons In1085 and In1086, containing the class D β-lactamase -encoding gene blaOXA, were identified in clinical enterobacterial strains. In this study, we aimed to characterize the genetic contexts of In1085 and In1086, with the goal of identifying putative mechanisms of integron mobilization. Four plasmids, approximately 5.3, 5.3, 5.7, and 6.6 kb, from 71 clinical Enterobacteriaceae strains were found to contain class 1 integrons (In37, In62, In1085, and In1086, respectively). Two of these plasmids, pEco336 and pNsa292, containing In1085 and In1086, respectively, were further characterized by antibiotic susceptibility testing, conjugation experiments, PCR, sequencing, and gene mapping. The OXA-type carbapenemase activities of the parental strains were also assessed. The results revealed that the novel integrons had different genetic environments, and therefore demonstrated diverse biochemical characteristics. Using evolutionary inferences based on the recombination of gene cassettes, we also identified a role for attCaadA16 structural features during attI1 × attC insertion reactions. Our analysis showed that gene cassette insertions in the bottom strand of attCaadA16 in the correct orientation lead to the expression the encoded genes from the Pc promoter. Our study suggests that the genetic features harbored within the integrons are inserted in a discernable pattern, involving the stepwise and parallel evolution of class 1 integron variations under antibiotic selection pressures in a clinical setting.http://journal.frontiersin.org/article/10.3389/fmicb.2017.01003/fullclass 1 integronIn1085In1086evolutionary inferencesgene cassettes |
| spellingShingle | Dongguo Wang Wei Hou Jiayu Chen Linjun Yang Zhihui Liu Zhe Yin Jiao Feng Dongsheng Zhou Characterization of Novel Integrons, In1085 and In1086, and the Surrounding Genes in Plasmids from Enterobacteriaceae, and the Role for attCaadA16 Structural Features during attI1 × attC Integration Frontiers in Microbiology class 1 integron In1085 In1086 evolutionary inferences gene cassettes |
| title | Characterization of Novel Integrons, In1085 and In1086, and the Surrounding Genes in Plasmids from Enterobacteriaceae, and the Role for attCaadA16 Structural Features during attI1 × attC Integration |
| title_full | Characterization of Novel Integrons, In1085 and In1086, and the Surrounding Genes in Plasmids from Enterobacteriaceae, and the Role for attCaadA16 Structural Features during attI1 × attC Integration |
| title_fullStr | Characterization of Novel Integrons, In1085 and In1086, and the Surrounding Genes in Plasmids from Enterobacteriaceae, and the Role for attCaadA16 Structural Features during attI1 × attC Integration |
| title_full_unstemmed | Characterization of Novel Integrons, In1085 and In1086, and the Surrounding Genes in Plasmids from Enterobacteriaceae, and the Role for attCaadA16 Structural Features during attI1 × attC Integration |
| title_short | Characterization of Novel Integrons, In1085 and In1086, and the Surrounding Genes in Plasmids from Enterobacteriaceae, and the Role for attCaadA16 Structural Features during attI1 × attC Integration |
| title_sort | characterization of novel integrons in1085 and in1086 and the surrounding genes in plasmids from enterobacteriaceae and the role for attcaada16 structural features during atti1 attc integration |
| topic | class 1 integron In1085 In1086 evolutionary inferences gene cassettes |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2017.01003/full |
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