LncRNA7467 participated in shell biomineralization in pearl oyster Pinctada fucata martensii
Long noncoding RNAs (LncRNAs) are reported to have multiple roles in various biological processes, including epigenetic regulation, transcriptional regulation and post-transcriptional regulation. However, the current studies for lncRNAs are mainly limited to mammalian species, and the investigations...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Aquaculture Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513422003945 |
| _version_ | 1797985377356611584 |
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| author | Hua Zhang Gaoyou Yao Maoxian He |
| author_facet | Hua Zhang Gaoyou Yao Maoxian He |
| author_sort | Hua Zhang |
| collection | DOAJ |
| description | Long noncoding RNAs (LncRNAs) are reported to have multiple roles in various biological processes, including epigenetic regulation, transcriptional regulation and post-transcriptional regulation. However, the current studies for lncRNAs are mainly limited to mammalian species, and the investigations of functional roles for lncRNAs in bivalve species are still lacking. Here, we identified a novel lncRNA named lncRNA7467 from pearl oyster Pinctada fucata martensii, located in chromosome 9 at 14962920–14963218 bp. Intriguingly, lncRNA7467 showed the highest expression level in mantle tissue among tested tissues, and responded to repair and regeneration of the injured shell. After knockdown gene expression by injecting lncRNA7467-specific double-stranded RNA, the inner surface of the prismatic layer changed significantly, and then the N44 gene was correspondingly inhibited. In addition, lncRNA7467 mainly distributed in the cytoplasm, and it mays primarily play a role in post-transcriptional regulation. These works indicated that lncRNA7467 was involved in the biomineralization process in pearl oyster P. f. martensii. |
| first_indexed | 2024-04-11T07:16:01Z |
| format | Article |
| id | doaj.art-d8b2d468ea614f77b89dae6ebc929c52 |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-04-11T07:16:01Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-d8b2d468ea614f77b89dae6ebc929c522022-12-22T04:37:56ZengElsevierAquaculture Reports2352-51342022-12-0127101398LncRNA7467 participated in shell biomineralization in pearl oyster Pinctada fucata martensiiHua Zhang0Gaoyou Yao1Maoxian He2CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, China; Innovation Academy of South China Sea Ecology and Environmental, Engineering, Chinese Academy of Sciences, Guangzhou 510301, ChinaCAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; University of Chinese Academy of Sciences, Beijing 100049, China; Innovation Academy of South China Sea Ecology and Environmental, Engineering, Chinese Academy of Sciences, Guangzhou 510301, ChinaCAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, China; Innovation Academy of South China Sea Ecology and Environmental, Engineering, Chinese Academy of Sciences, Guangzhou 510301, China; Correspondence to: Key Laboratory of Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China.Long noncoding RNAs (LncRNAs) are reported to have multiple roles in various biological processes, including epigenetic regulation, transcriptional regulation and post-transcriptional regulation. However, the current studies for lncRNAs are mainly limited to mammalian species, and the investigations of functional roles for lncRNAs in bivalve species are still lacking. Here, we identified a novel lncRNA named lncRNA7467 from pearl oyster Pinctada fucata martensii, located in chromosome 9 at 14962920–14963218 bp. Intriguingly, lncRNA7467 showed the highest expression level in mantle tissue among tested tissues, and responded to repair and regeneration of the injured shell. After knockdown gene expression by injecting lncRNA7467-specific double-stranded RNA, the inner surface of the prismatic layer changed significantly, and then the N44 gene was correspondingly inhibited. In addition, lncRNA7467 mainly distributed in the cytoplasm, and it mays primarily play a role in post-transcriptional regulation. These works indicated that lncRNA7467 was involved in the biomineralization process in pearl oyster P. f. martensii.http://www.sciencedirect.com/science/article/pii/S2352513422003945Pinctada fucata martensiiLncRNA7467Gene expressionBiomineralization |
| spellingShingle | Hua Zhang Gaoyou Yao Maoxian He LncRNA7467 participated in shell biomineralization in pearl oyster Pinctada fucata martensii Aquaculture Reports Pinctada fucata martensii LncRNA7467 Gene expression Biomineralization |
| title | LncRNA7467 participated in shell biomineralization in pearl oyster Pinctada fucata martensii |
| title_full | LncRNA7467 participated in shell biomineralization in pearl oyster Pinctada fucata martensii |
| title_fullStr | LncRNA7467 participated in shell biomineralization in pearl oyster Pinctada fucata martensii |
| title_full_unstemmed | LncRNA7467 participated in shell biomineralization in pearl oyster Pinctada fucata martensii |
| title_short | LncRNA7467 participated in shell biomineralization in pearl oyster Pinctada fucata martensii |
| title_sort | lncrna7467 participated in shell biomineralization in pearl oyster pinctada fucata martensii |
| topic | Pinctada fucata martensii LncRNA7467 Gene expression Biomineralization |
| url | http://www.sciencedirect.com/science/article/pii/S2352513422003945 |
| work_keys_str_mv | AT huazhang lncrna7467participatedinshellbiomineralizationinpearloysterpinctadafucatamartensii AT gaoyouyao lncrna7467participatedinshellbiomineralizationinpearloysterpinctadafucatamartensii AT maoxianhe lncrna7467participatedinshellbiomineralizationinpearloysterpinctadafucatamartensii |