Development of a Simple Protocol for Zymogram-Based Isolation and Characterization of Gingipains from <i>Porphyromonas gingivalis</i>: The Causative Agent of Periodontitis

Development of a Simple Protocol for Zymogram-Based Isolation and Characterization of Gingipains from <i>Porphyromonas gingivalis</i>: The Causative Agent of Periodontitis

Gingipains (RgpA, RgpB, and Kgp) are major virulence factors of the periodontitis-causing bacterium <i>Porphyromonas gingivalis</i>. Isolation of gingipains from the crude protein sample of <i>P. gingivalis</i> is critical for studying the underlying invasion mechanism that c...

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Bibliographic Details
Main Authors: Eng Sze Wei, Ramasamy Kavitha, Mohammad Auwal Sa’ad, Pattabhiraman Lalitha, Neeraj Kumar Fuloria, Manickam Ravichandran, Shivkanya Fuloria
Format: Article
Language:English
Published: MDPI AG 2023-03-01
Series:Applied Sciences
Subjects:
isolation
characterization
zymography
gingipains
<i>Porphyromonas gingivalis</i>
Rgp
Online Access:https://www.mdpi.com/2076-3417/13/7/4314

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