Expression of different L1 isoforms of Mastomys natalensis papillomavirus as mechanism to circumvent adaptive immunity
Although many high-risk mucosal and cutaneous human papillomaviruses (HPVs) theoretically have the potential to synthesize L1 isoforms differing in length, previous seroepidemiological studies only focused on the short L1 variants, co-assembling with L2 to infectious virions. Using the multimammate...
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2020-08-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/57626 |
| _version_ | 1811236001096400896 |
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| author | Yingying Fu Rui Cao Miriam Schäfer Sonja Stephan Ilona Braspenning-Wesch Laura Schmitt Ralf Bischoff Martin Müller Kai Schäfer Sabrina E Vinzón Frank Rösl Daniel Hasche |
| author_facet | Yingying Fu Rui Cao Miriam Schäfer Sonja Stephan Ilona Braspenning-Wesch Laura Schmitt Ralf Bischoff Martin Müller Kai Schäfer Sabrina E Vinzón Frank Rösl Daniel Hasche |
| author_sort | Yingying Fu |
| collection | DOAJ |
| description | Although many high-risk mucosal and cutaneous human papillomaviruses (HPVs) theoretically have the potential to synthesize L1 isoforms differing in length, previous seroepidemiological studies only focused on the short L1 variants, co-assembling with L2 to infectious virions. Using the multimammate mouse Mastomys coucha as preclinical model, this is the first study demonstrating seroconversion against different L1 isoforms during the natural course of papillomavirus infection. Intriguingly, positivity with the cutaneous MnPV was accompanied by a strong seroresponse against a longer L1 isoform, but to our surprise, the raised antibodies were non-neutralizing. Only after a delay of around 4 months, protecting antibodies against the short L1 appeared, enabling the virus to successfully establish an infection. This argues for a novel humoral immune escape mechanism that may also have important implications on the interpretation of epidemiological data in terms of seropositivity and protection of PV infections in general. |
| first_indexed | 2024-04-12T12:01:52Z |
| format | Article |
| id | doaj.art-d8b9e86c43164c898431dd97d6ecd671 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T12:01:52Z |
| publishDate | 2020-08-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-d8b9e86c43164c898431dd97d6ecd6712022-12-22T03:33:50ZengeLife Sciences Publications LtdeLife2050-084X2020-08-01910.7554/eLife.57626Expression of different L1 isoforms of Mastomys natalensis papillomavirus as mechanism to circumvent adaptive immunityYingying Fu0Rui Cao1Miriam Schäfer2Sonja Stephan3Ilona Braspenning-Wesch4Laura Schmitt5Ralf Bischoff6Martin Müller7Kai Schäfer8Sabrina E Vinzón9https://orcid.org/0000-0002-1330-9125Frank Rösl10Daniel Hasche11https://orcid.org/0000-0001-9306-3059Division of Viral Transformation Mechanisms, Research Program 'Infection, Inflammation and Cancer', German Cancer Research Center, Heidelberg, GermanyDivision of Viral Transformation Mechanisms, Research Program 'Infection, Inflammation and Cancer', German Cancer Research Center, Heidelberg, GermanyDivision of Viral Transformation Mechanisms, Research Program 'Infection, Inflammation and Cancer', German Cancer Research Center, Heidelberg, GermanyDivision of Viral Transformation Mechanisms, Research Program 'Infection, Inflammation and Cancer', German Cancer Research Center, Heidelberg, GermanyDivision of Viral Transformation Mechanisms, Research Program 'Infection, Inflammation and Cancer', German Cancer Research Center, Heidelberg, GermanyDivision of Viral Transformation Mechanisms, Research Program 'Infection, Inflammation and Cancer', German Cancer Research Center, Heidelberg, GermanyDivision of Functional Genome Analysis, Research Program 'Functional and Structural Genomics', German Cancer Research Center, Heidelberg, GermanyResearch Group Tumorvirus-specific Vaccination Strategies, Research Program 'Infection, Inflammation and Cancer', German Cancer Research Center, Heidelberg, GermanyDivision of Viral Transformation Mechanisms, Research Program 'Infection, Inflammation and Cancer', German Cancer Research Center, Heidelberg, GermanyDivision of Viral Transformation Mechanisms, Research Program 'Infection, Inflammation and Cancer', German Cancer Research Center, Heidelberg, GermanyDivision of Viral Transformation Mechanisms, Research Program 'Infection, Inflammation and Cancer', German Cancer Research Center, Heidelberg, GermanyDivision of Viral Transformation Mechanisms, Research Program 'Infection, Inflammation and Cancer', German Cancer Research Center, Heidelberg, GermanyAlthough many high-risk mucosal and cutaneous human papillomaviruses (HPVs) theoretically have the potential to synthesize L1 isoforms differing in length, previous seroepidemiological studies only focused on the short L1 variants, co-assembling with L2 to infectious virions. Using the multimammate mouse Mastomys coucha as preclinical model, this is the first study demonstrating seroconversion against different L1 isoforms during the natural course of papillomavirus infection. Intriguingly, positivity with the cutaneous MnPV was accompanied by a strong seroresponse against a longer L1 isoform, but to our surprise, the raised antibodies were non-neutralizing. Only after a delay of around 4 months, protecting antibodies against the short L1 appeared, enabling the virus to successfully establish an infection. This argues for a novel humoral immune escape mechanism that may also have important implications on the interpretation of epidemiological data in terms of seropositivity and protection of PV infections in general.https://elifesciences.org/articles/57626Mastomys couchapapillomavirusimmune escapeseroconversionneutralizationvirus-like particle |
| spellingShingle | Yingying Fu Rui Cao Miriam Schäfer Sonja Stephan Ilona Braspenning-Wesch Laura Schmitt Ralf Bischoff Martin Müller Kai Schäfer Sabrina E Vinzón Frank Rösl Daniel Hasche Expression of different L1 isoforms of Mastomys natalensis papillomavirus as mechanism to circumvent adaptive immunity eLife Mastomys coucha papillomavirus immune escape seroconversion neutralization virus-like particle |
| title | Expression of different L1 isoforms of Mastomys natalensis papillomavirus as mechanism to circumvent adaptive immunity |
| title_full | Expression of different L1 isoforms of Mastomys natalensis papillomavirus as mechanism to circumvent adaptive immunity |
| title_fullStr | Expression of different L1 isoforms of Mastomys natalensis papillomavirus as mechanism to circumvent adaptive immunity |
| title_full_unstemmed | Expression of different L1 isoforms of Mastomys natalensis papillomavirus as mechanism to circumvent adaptive immunity |
| title_short | Expression of different L1 isoforms of Mastomys natalensis papillomavirus as mechanism to circumvent adaptive immunity |
| title_sort | expression of different l1 isoforms of mastomys natalensis papillomavirus as mechanism to circumvent adaptive immunity |
| topic | Mastomys coucha papillomavirus immune escape seroconversion neutralization virus-like particle |
| url | https://elifesciences.org/articles/57626 |
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