Development of 10 single‐copy nuclear DNA markers for Euchresta horsfieldii (Fabaceae), a rare medicinal plant
Premise of the Study Euchresta horsfieldii (Fabaceae) is a rare and endangered medicinal plant in Indonesia with restricted distribution. Single‐copy nuclear DNA (scnDNA) markers were developed for this species to facilitate further investigation of genetic diversity and population structure. Method...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
Wiley
2018-09-01
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Series: | Applications in Plant Sciences |
Subjects: | |
Online Access: | https://doi.org/10.1002/aps3.1178 |
Summary: | Premise of the Study Euchresta horsfieldii (Fabaceae) is a rare and endangered medicinal plant in Indonesia with restricted distribution. Single‐copy nuclear DNA (scnDNA) markers were developed for this species to facilitate further investigation of genetic diversity and population structure. Methods and Results We performed RNA‐Seq and de novo assembly of the transcriptome. Ten primer sets were developed for E. horsfieldii, all of which also amplified in E. japonica and E. tubulosa. Conclusions These scnDNA markers will be an important resource for the study of genetic diversity and population structure of E. horsfieldii and other species in the genus Euchresta. |
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ISSN: | 2168-0450 |