Development of 10 single‐copy nuclear DNA markers for Euchresta horsfieldii (Fabaceae), a rare medicinal plant
Premise of the Study Euchresta horsfieldii (Fabaceae) is a rare and endangered medicinal plant in Indonesia with restricted distribution. Single‐copy nuclear DNA (scnDNA) markers were developed for this species to facilitate further investigation of genetic diversity and population structure. Method...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2018-09-01
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| Series: | Applications in Plant Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/aps3.1178 |
| _version_ | 1811226375821983744 |
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| author | Arief Priyadi Chao Feng Ming Kang Hongwen Huang |
| author_facet | Arief Priyadi Chao Feng Ming Kang Hongwen Huang |
| author_sort | Arief Priyadi |
| collection | DOAJ |
| description | Premise of the Study Euchresta horsfieldii (Fabaceae) is a rare and endangered medicinal plant in Indonesia with restricted distribution. Single‐copy nuclear DNA (scnDNA) markers were developed for this species to facilitate further investigation of genetic diversity and population structure. Methods and Results We performed RNA‐Seq and de novo assembly of the transcriptome. Ten primer sets were developed for E. horsfieldii, all of which also amplified in E. japonica and E. tubulosa. Conclusions These scnDNA markers will be an important resource for the study of genetic diversity and population structure of E. horsfieldii and other species in the genus Euchresta. |
| first_indexed | 2024-04-12T09:24:04Z |
| format | Article |
| id | doaj.art-d8ba83214b6c41bdabe7698a95bb9904 |
| institution | Directory Open Access Journal |
| issn | 2168-0450 |
| language | English |
| last_indexed | 2024-04-12T09:24:04Z |
| publishDate | 2018-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Applications in Plant Sciences |
| spelling | doaj.art-d8ba83214b6c41bdabe7698a95bb99042022-12-22T03:38:33ZengWileyApplications in Plant Sciences2168-04502018-09-0169n/an/a10.1002/aps3.1178Development of 10 single‐copy nuclear DNA markers for Euchresta horsfieldii (Fabaceae), a rare medicinal plantArief Priyadi0Chao Feng1Ming Kang2Hongwen Huang3Key Laboratory of Plant Resources Conservation and Sustainable Utilization South China Botanical Garden Chinese Academy of Sciences Guangzhou 510650 People's Republic of ChinaKey Laboratory of Plant Resources Conservation and Sustainable Utilization South China Botanical Garden Chinese Academy of Sciences Guangzhou 510650 People's Republic of ChinaKey Laboratory of Plant Resources Conservation and Sustainable Utilization South China Botanical Garden Chinese Academy of Sciences Guangzhou 510650 People's Republic of ChinaKey Laboratory of Plant Resources Conservation and Sustainable Utilization South China Botanical Garden Chinese Academy of Sciences Guangzhou 510650 People's Republic of ChinaPremise of the Study Euchresta horsfieldii (Fabaceae) is a rare and endangered medicinal plant in Indonesia with restricted distribution. Single‐copy nuclear DNA (scnDNA) markers were developed for this species to facilitate further investigation of genetic diversity and population structure. Methods and Results We performed RNA‐Seq and de novo assembly of the transcriptome. Ten primer sets were developed for E. horsfieldii, all of which also amplified in E. japonica and E. tubulosa. Conclusions These scnDNA markers will be an important resource for the study of genetic diversity and population structure of E. horsfieldii and other species in the genus Euchresta.https://doi.org/10.1002/aps3.1178Euchresta horsfieldiiFabaceaegenetic diversityRNA‐Seqsingle‐copy nuclear DNA (scnDNA) markers |
| spellingShingle | Arief Priyadi Chao Feng Ming Kang Hongwen Huang Development of 10 single‐copy nuclear DNA markers for Euchresta horsfieldii (Fabaceae), a rare medicinal plant Applications in Plant Sciences Euchresta horsfieldii Fabaceae genetic diversity RNA‐Seq single‐copy nuclear DNA (scnDNA) markers |
| title | Development of 10 single‐copy nuclear DNA markers for Euchresta horsfieldii (Fabaceae), a rare medicinal plant |
| title_full | Development of 10 single‐copy nuclear DNA markers for Euchresta horsfieldii (Fabaceae), a rare medicinal plant |
| title_fullStr | Development of 10 single‐copy nuclear DNA markers for Euchresta horsfieldii (Fabaceae), a rare medicinal plant |
| title_full_unstemmed | Development of 10 single‐copy nuclear DNA markers for Euchresta horsfieldii (Fabaceae), a rare medicinal plant |
| title_short | Development of 10 single‐copy nuclear DNA markers for Euchresta horsfieldii (Fabaceae), a rare medicinal plant |
| title_sort | development of 10 single copy nuclear dna markers for euchresta horsfieldii fabaceae a rare medicinal plant |
| topic | Euchresta horsfieldii Fabaceae genetic diversity RNA‐Seq single‐copy nuclear DNA (scnDNA) markers |
| url | https://doi.org/10.1002/aps3.1178 |
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