Identification of Novel miRNAs and Their Target Genes in the Response to Abscisic Acid in <i>Arabidopsis</i>

Identification of Novel miRNAs and Their Target Genes in the Response to Abscisic Acid in <i>Arabidopsis</i>

miRNAs are involved in various biological processes, including adaptive responses to abiotic stress. To understand the role of miRNAs in the response to ABA, ABA-responsive miRNAs were identified by small RNA sequencing in wild-type <i>Arabidopsis</i>, as well as in <i>abi1td</i...

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Bibliographic Details
Main Authors: Syed Muhammad Muntazir Mehdi, Sivakumar Krishnamoorthy, Michal Wojciech Szczesniak, Agnieszka Ludwików
Format: Article
Language:English
Published: MDPI AG 2021-07-01
Series:International Journal of Molecular Sciences
Subjects:
miRNA
abscisic acid
ABI1 PP2C
MAPKKK17
MAPKKK18
<i>Arabidopsis thaliana</i>
Online Access:https://www.mdpi.com/1422-0067/22/13/7153
Description
Summary:miRNAs are involved in various biological processes, including adaptive responses to abiotic stress. To understand the role of miRNAs in the response to ABA, ABA-responsive miRNAs were identified by small RNA sequencing in wild-type <i>Arabidopsis</i>, as well as in <i>abi1td</i>, <i>mkkk17</i>, and <i>mkkk18</i> mutants. We identified 10 novel miRNAs in WT after ABA treatment, while in <i>abi1td</i>, <i>mkkk17</i>, and <i>mkkk18</i> mutants, three, seven, and nine known miRNAs, respectively, were differentially expressed after ABA treatment. One novel miRNA (miRn-8) was differentially expressed in the <i>mkkk17</i> mutant. Potential target genes of the miRNA panel were identified using psRNATarget. Sequencing results were validated by quantitative RT-PCR of several known and novel miRNAs in all genotypes. Of the predicted targets of novel miRNAs, seven target genes of six novel miRNAs were further validated by 5′ RLM-RACE. Gene ontology analyses showed the potential target genes of ABA-responsive known and novel miRNAs to be involved in diverse cellular processes in plants, including development and stomatal movement. These outcomes suggest that a number of the identified miRNAs have crucial roles in plant responses to environmental stress, as well as in plant development, and might have common regulatory roles in the core ABA signaling pathway.
ISSN:1661-6596
1422-0067

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