Learning from magnetotactic bacteria: mms6 protects stem cells from oxidative damage
Oxidative damage generally exists in stroke and impairs stem cells’ survival; however, the problem is difficult to treat. In order to help stem cells to resist this damage, we inserted a magnetotactic bacteria (MB) gene, mms6, into the neural stem cell genome by lentiviral transfection. It was found...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Cellular Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fncel.2022.1075640/full |
| _version_ | 1811217469516283904 |
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| author | Nai-Li Wei Nai-Li Wei Wenjing Xu Wenjing Xu Hai-Liang Tang Qiang Xie Yuting Zhai Yuting Zhai Jian Chen Xiao-Yong Zhang Xiao-Yong Zhang Jian-Hong Zhu |
| author_facet | Nai-Li Wei Nai-Li Wei Wenjing Xu Wenjing Xu Hai-Liang Tang Qiang Xie Yuting Zhai Yuting Zhai Jian Chen Xiao-Yong Zhang Xiao-Yong Zhang Jian-Hong Zhu |
| author_sort | Nai-Li Wei |
| collection | DOAJ |
| description | Oxidative damage generally exists in stroke and impairs stem cells’ survival; however, the problem is difficult to treat. In order to help stem cells to resist this damage, we inserted a magnetotactic bacteria (MB) gene, mms6, into the neural stem cell genome by lentiviral transfection. It was found that the transfection of mms6 significantly improved the survival rate of stem cells in the condition of iron overload but not hypoxia. The bioenergetic profile also revealed that iron overloading weakened the mitochondrial respiration and spare respiration capacity of stem cells, but that these were enhanced after the expression of mms6. Additionally, Western blotting (WB) data revealed that mms6 upregulated the expression of glutathione peroxidase (GPX4), which protected stem cells from oxidative damage and ferroptosis. In order to determine the possible mechanisms, we analyzed the interactions between the MMS6 protein, Fe2+, and GPX4 via analog computation. The predicted models found that the MMS6 protein had a direct chelating site in the region of M6A with divalent iron; it also had weak binding with GPX4. Taken together, the magnetotactic bacterial gene mms6 protected stem cells from oxidative damage via binding with Fe2+, which could help them adapt to the microenvironment of stroke. |
| first_indexed | 2024-04-12T06:55:19Z |
| format | Article |
| id | doaj.art-d8bc67d5938549aaaef4625a3ae1f381 |
| institution | Directory Open Access Journal |
| issn | 1662-5102 |
| language | English |
| last_indexed | 2024-04-12T06:55:19Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular Neuroscience |
| spelling | doaj.art-d8bc67d5938549aaaef4625a3ae1f3812022-12-22T03:43:11ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022022-11-011610.3389/fncel.2022.10756401075640Learning from magnetotactic bacteria: mms6 protects stem cells from oxidative damageNai-Li Wei0Nai-Li Wei1Wenjing Xu2Wenjing Xu3Hai-Liang Tang4Qiang Xie5Yuting Zhai6Yuting Zhai7Jian Chen8Xiao-Yong Zhang9Xiao-Yong Zhang10Jian-Hong Zhu11Department of Neurosurgery, The First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, ChinaState Key Laboratory for Medical Neurobiology, Department of Neurosurgery, Institutes of Brain Science, Fudan University Huashan Hospital, Shanghai Medical College-Fudan University, Shanghai, ChinaInstitute of Science and Technology for Brain-Inspired Intelligence, Fudan University, Shanghai, ChinaMOE Key Laboratory of Computational Neuroscience and Brain-Inspired Intelligence, MOE Frontiers Center for Brain Science, Fudan University, Shanghai, ChinaState Key Laboratory for Medical Neurobiology, Department of Neurosurgery, Institutes of Brain Science, Fudan University Huashan Hospital, Shanghai Medical College-Fudan University, Shanghai, ChinaDepartment of Neurosurgery, Fudan University Zhongshan Hospital, Shanghai Medical College of Fudan University, Shanghai, ChinaInstitute of Science and Technology for Brain-Inspired Intelligence, Fudan University, Shanghai, ChinaMOE Key Laboratory of Computational Neuroscience and Brain-Inspired Intelligence, MOE Frontiers Center for Brain Science, Fudan University, Shanghai, ChinaDepartment of Neurosurgery, The First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, ChinaInstitute of Science and Technology for Brain-Inspired Intelligence, Fudan University, Shanghai, ChinaMOE Key Laboratory of Computational Neuroscience and Brain-Inspired Intelligence, MOE Frontiers Center for Brain Science, Fudan University, Shanghai, ChinaState Key Laboratory for Medical Neurobiology, Department of Neurosurgery, Institutes of Brain Science, Fudan University Huashan Hospital, Shanghai Medical College-Fudan University, Shanghai, ChinaOxidative damage generally exists in stroke and impairs stem cells’ survival; however, the problem is difficult to treat. In order to help stem cells to resist this damage, we inserted a magnetotactic bacteria (MB) gene, mms6, into the neural stem cell genome by lentiviral transfection. It was found that the transfection of mms6 significantly improved the survival rate of stem cells in the condition of iron overload but not hypoxia. The bioenergetic profile also revealed that iron overloading weakened the mitochondrial respiration and spare respiration capacity of stem cells, but that these were enhanced after the expression of mms6. Additionally, Western blotting (WB) data revealed that mms6 upregulated the expression of glutathione peroxidase (GPX4), which protected stem cells from oxidative damage and ferroptosis. In order to determine the possible mechanisms, we analyzed the interactions between the MMS6 protein, Fe2+, and GPX4 via analog computation. The predicted models found that the MMS6 protein had a direct chelating site in the region of M6A with divalent iron; it also had weak binding with GPX4. Taken together, the magnetotactic bacterial gene mms6 protected stem cells from oxidative damage via binding with Fe2+, which could help them adapt to the microenvironment of stroke.https://www.frontiersin.org/articles/10.3389/fncel.2022.1075640/fullstem cellsreactive oxygen speciesmms6antioxidantmitochondrial function |
| spellingShingle | Nai-Li Wei Nai-Li Wei Wenjing Xu Wenjing Xu Hai-Liang Tang Qiang Xie Yuting Zhai Yuting Zhai Jian Chen Xiao-Yong Zhang Xiao-Yong Zhang Jian-Hong Zhu Learning from magnetotactic bacteria: mms6 protects stem cells from oxidative damage Frontiers in Cellular Neuroscience stem cells reactive oxygen species mms6 antioxidant mitochondrial function |
| title | Learning from magnetotactic bacteria: mms6 protects stem cells from oxidative damage |
| title_full | Learning from magnetotactic bacteria: mms6 protects stem cells from oxidative damage |
| title_fullStr | Learning from magnetotactic bacteria: mms6 protects stem cells from oxidative damage |
| title_full_unstemmed | Learning from magnetotactic bacteria: mms6 protects stem cells from oxidative damage |
| title_short | Learning from magnetotactic bacteria: mms6 protects stem cells from oxidative damage |
| title_sort | learning from magnetotactic bacteria mms6 protects stem cells from oxidative damage |
| topic | stem cells reactive oxygen species mms6 antioxidant mitochondrial function |
| url | https://www.frontiersin.org/articles/10.3389/fncel.2022.1075640/full |
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