Specific Expression of Antimicrobial Peptides from the Black Soldier Fly in the Midgut of Silkworms (<i>Bombyx mori</i>) Regulates Silkworm Immunity
Antimicrobial peptides are molecules with strong antimicrobial activity and are of substantial interest for the immunization of insects. As a type of dipteran insect that can turn organic waste into animal feed, the black soldier fly (BSF) can “turn waste into treasure”. In this study, we investigat...
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MDPI AG
2023-05-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/14/5/443 |
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| author | Xuan Deng Lianlian Liu Jing Deng Xingfu Zha |
| author_facet | Xuan Deng Lianlian Liu Jing Deng Xingfu Zha |
| author_sort | Xuan Deng |
| collection | DOAJ |
| description | Antimicrobial peptides are molecules with strong antimicrobial activity and are of substantial interest for the immunization of insects. As a type of dipteran insect that can turn organic waste into animal feed, the black soldier fly (BSF) can “turn waste into treasure”. In this study, we investigated the antimicrobial activity of the antimicrobial peptide genes, <i>HiCG13551</i> and <i>Hidiptericin-1,</i> of BSF in silkworms, by overexpressing the genes specifically in the midgut. Changes in the mRNA levels of the transgenic silkworms after infection with <i>Staphylococcus aureus</i> were evaluated using transcriptome sequencing. The results showed that <i>Hidiptericin-1</i> had stronger antimicrobial activity than <i>HiCG13551</i>. KEGG enrichment analysis showed that the differentially expressed genes in the transgenic overexpressed <i>Hidiptericin-1</i> silkworm lines from the D9L strain were mainly enriched in the starch and sucrose metabolism, pantothenate and CoA biosynthesis, drug metabolism (other enzymes), biotin metabolism, platinum drug resistance, galactose metabolism, and pancreatic secretion pathways. In addition, immune-related genes were up-regulated in this transgenic silkworm strain. Our study may provide new insights for future immune studies on insects. |
| first_indexed | 2024-03-11T03:39:00Z |
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| id | doaj.art-d8c0b75804e04160a2774a0232c51f6d |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-11T03:39:00Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-d8c0b75804e04160a2774a0232c51f6d2023-11-18T01:50:00ZengMDPI AGInsects2075-44502023-05-0114544310.3390/insects14050443Specific Expression of Antimicrobial Peptides from the Black Soldier Fly in the Midgut of Silkworms (<i>Bombyx mori</i>) Regulates Silkworm ImmunityXuan Deng0Lianlian Liu1Jing Deng2Xingfu Zha3State Key Laboratory of Resource Insects, College of Sericulture, Textile and Biomass Sciences, Southwest University, Chongqing 400715, ChinaState Key Laboratory of Resource Insects, College of Sericulture, Textile and Biomass Sciences, Southwest University, Chongqing 400715, ChinaState Key Laboratory of Resource Insects, College of Sericulture, Textile and Biomass Sciences, Southwest University, Chongqing 400715, ChinaState Key Laboratory of Resource Insects, College of Sericulture, Textile and Biomass Sciences, Southwest University, Chongqing 400715, ChinaAntimicrobial peptides are molecules with strong antimicrobial activity and are of substantial interest for the immunization of insects. As a type of dipteran insect that can turn organic waste into animal feed, the black soldier fly (BSF) can “turn waste into treasure”. In this study, we investigated the antimicrobial activity of the antimicrobial peptide genes, <i>HiCG13551</i> and <i>Hidiptericin-1,</i> of BSF in silkworms, by overexpressing the genes specifically in the midgut. Changes in the mRNA levels of the transgenic silkworms after infection with <i>Staphylococcus aureus</i> were evaluated using transcriptome sequencing. The results showed that <i>Hidiptericin-1</i> had stronger antimicrobial activity than <i>HiCG13551</i>. KEGG enrichment analysis showed that the differentially expressed genes in the transgenic overexpressed <i>Hidiptericin-1</i> silkworm lines from the D9L strain were mainly enriched in the starch and sucrose metabolism, pantothenate and CoA biosynthesis, drug metabolism (other enzymes), biotin metabolism, platinum drug resistance, galactose metabolism, and pancreatic secretion pathways. In addition, immune-related genes were up-regulated in this transgenic silkworm strain. Our study may provide new insights for future immune studies on insects.https://www.mdpi.com/2075-4450/14/5/443transgenic silkwormmidgutblack soldier flyantimicrobial peptidetranscriptome sequencing |
| spellingShingle | Xuan Deng Lianlian Liu Jing Deng Xingfu Zha Specific Expression of Antimicrobial Peptides from the Black Soldier Fly in the Midgut of Silkworms (<i>Bombyx mori</i>) Regulates Silkworm Immunity Insects transgenic silkworm midgut black soldier fly antimicrobial peptide transcriptome sequencing |
| title | Specific Expression of Antimicrobial Peptides from the Black Soldier Fly in the Midgut of Silkworms (<i>Bombyx mori</i>) Regulates Silkworm Immunity |
| title_full | Specific Expression of Antimicrobial Peptides from the Black Soldier Fly in the Midgut of Silkworms (<i>Bombyx mori</i>) Regulates Silkworm Immunity |
| title_fullStr | Specific Expression of Antimicrobial Peptides from the Black Soldier Fly in the Midgut of Silkworms (<i>Bombyx mori</i>) Regulates Silkworm Immunity |
| title_full_unstemmed | Specific Expression of Antimicrobial Peptides from the Black Soldier Fly in the Midgut of Silkworms (<i>Bombyx mori</i>) Regulates Silkworm Immunity |
| title_short | Specific Expression of Antimicrobial Peptides from the Black Soldier Fly in the Midgut of Silkworms (<i>Bombyx mori</i>) Regulates Silkworm Immunity |
| title_sort | specific expression of antimicrobial peptides from the black soldier fly in the midgut of silkworms i bombyx mori i regulates silkworm immunity |
| topic | transgenic silkworm midgut black soldier fly antimicrobial peptide transcriptome sequencing |
| url | https://www.mdpi.com/2075-4450/14/5/443 |
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