Prevalence, species diversity, and antimicrobial susceptibility of Campylobacter strains in patients with diarrhea and poultry meat samples: one-year prospective study
Background and Objectives: Source tracking of antimicrobial resistance in Campylobacter is useful for control measures. In this study, Campylobacter-associated diarrhea and homology in antimicrobial resistance of humans and poultry meat isolates were investigated. Materials and Methods: A total of...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2022-06-01
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| Series: | Iranian Journal of Microbiology |
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| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/3520 |
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| author | Atena Sadeghi Leila Ganji Fereshteh Fani Gholamreza Pouladfar Parisa Eslami Fatemeh Doregiraee Parviz Owlia Masoud Alebouyeh |
| author_facet | Atena Sadeghi Leila Ganji Fereshteh Fani Gholamreza Pouladfar Parisa Eslami Fatemeh Doregiraee Parviz Owlia Masoud Alebouyeh |
| author_sort | Atena Sadeghi |
| collection | DOAJ |
| description |
Background and Objectives: Source tracking of antimicrobial resistance in Campylobacter is useful for control measures. In this study, Campylobacter-associated diarrhea and homology in antimicrobial resistance of humans and poultry meat isolates were investigated.
Materials and Methods: A total of 400 stools of patients and 100 poultry meat samples were analyzed. Susceptibility of the isolates was detected by disk diffusion, Etest, and agar dilution methods. Mismatch amplification mutation assay was used for the detection of mutations in the gyrA quinolone resistance determining region (QRDR).
Results: Campylobacter spp., including C. jejuni, C. coli, and C. lari, were detected in 35% of the chicken meat and 6.75% of the stool samples, respectively. The QRDR mutation was detected in most of the stool and chicken meat samples. Although the frequency of resistance to tetracycline (53.5% and 62.8%), erythromycin (39.2% and 37.1%), and gentamicin (32.1% and 31.4%) was relatively similar, higher frequency of resistance to ciprofloxacin (51.4% vs 28.6%) and nalidixic acid (42.15% vs 28.6%) among the chicken meat, and ampicillin (50% and 17.1%) among the human stool was detected.
Conclusion: High percentage of poultry meat samples is contaminated with different Campylobacter species, which shows homology with the patients’ isolates in Tehran.
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| first_indexed | 2024-04-13T18:59:36Z |
| format | Article |
| id | doaj.art-d8c12f4358e548edbbdae9651dd56d9b |
| institution | Directory Open Access Journal |
| issn | 2008-3289 2008-4447 |
| language | English |
| last_indexed | 2024-04-13T18:59:36Z |
| publishDate | 2022-06-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj.art-d8c12f4358e548edbbdae9651dd56d9b2022-12-22T02:34:09ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472022-06-01143Prevalence, species diversity, and antimicrobial susceptibility of Campylobacter strains in patients with diarrhea and poultry meat samples: one-year prospective studyAtena Sadeghi0Leila Ganji1Fereshteh Fani2Gholamreza Pouladfar3Parisa Eslami4Fatemeh Doregiraee5Parviz Owlia6Masoud Alebouyeh7Department of Microbiology, Faculty of Medicine, Shahed University, Tehran, IranDepartment of Microbiology, Iranian Reference Health Laboratory Research Center, Ministry of Health and Medical Education, Tehran, IranProfessor Alborzi Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, IranProfessor Alborzi Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Microbiology, Central Laboratory, Milad Hospital, Tehran, IranDepartment of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, IranMolecular Microbiology Research Center, Faculty of Medicine, Shahed University, Tehran, IranPediatric Infections Research Center, Research Institute for Children’s Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran Background and Objectives: Source tracking of antimicrobial resistance in Campylobacter is useful for control measures. In this study, Campylobacter-associated diarrhea and homology in antimicrobial resistance of humans and poultry meat isolates were investigated. Materials and Methods: A total of 400 stools of patients and 100 poultry meat samples were analyzed. Susceptibility of the isolates was detected by disk diffusion, Etest, and agar dilution methods. Mismatch amplification mutation assay was used for the detection of mutations in the gyrA quinolone resistance determining region (QRDR). Results: Campylobacter spp., including C. jejuni, C. coli, and C. lari, were detected in 35% of the chicken meat and 6.75% of the stool samples, respectively. The QRDR mutation was detected in most of the stool and chicken meat samples. Although the frequency of resistance to tetracycline (53.5% and 62.8%), erythromycin (39.2% and 37.1%), and gentamicin (32.1% and 31.4%) was relatively similar, higher frequency of resistance to ciprofloxacin (51.4% vs 28.6%) and nalidixic acid (42.15% vs 28.6%) among the chicken meat, and ampicillin (50% and 17.1%) among the human stool was detected. Conclusion: High percentage of poultry meat samples is contaminated with different Campylobacter species, which shows homology with the patients’ isolates in Tehran. https://ijm.tums.ac.ir/index.php/ijm/article/view/3520Foodborne diseases;Campylobacter;Drug resistance;Diarrhea;Poultry |
| spellingShingle | Atena Sadeghi Leila Ganji Fereshteh Fani Gholamreza Pouladfar Parisa Eslami Fatemeh Doregiraee Parviz Owlia Masoud Alebouyeh Prevalence, species diversity, and antimicrobial susceptibility of Campylobacter strains in patients with diarrhea and poultry meat samples: one-year prospective study Iranian Journal of Microbiology Foodborne diseases; Campylobacter; Drug resistance; Diarrhea; Poultry |
| title | Prevalence, species diversity, and antimicrobial susceptibility of Campylobacter strains in patients with diarrhea and poultry meat samples: one-year prospective study |
| title_full | Prevalence, species diversity, and antimicrobial susceptibility of Campylobacter strains in patients with diarrhea and poultry meat samples: one-year prospective study |
| title_fullStr | Prevalence, species diversity, and antimicrobial susceptibility of Campylobacter strains in patients with diarrhea and poultry meat samples: one-year prospective study |
| title_full_unstemmed | Prevalence, species diversity, and antimicrobial susceptibility of Campylobacter strains in patients with diarrhea and poultry meat samples: one-year prospective study |
| title_short | Prevalence, species diversity, and antimicrobial susceptibility of Campylobacter strains in patients with diarrhea and poultry meat samples: one-year prospective study |
| title_sort | prevalence species diversity and antimicrobial susceptibility of campylobacter strains in patients with diarrhea and poultry meat samples one year prospective study |
| topic | Foodborne diseases; Campylobacter; Drug resistance; Diarrhea; Poultry |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/3520 |
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