A green single-step procedure to synthesize Ag-containing nanocomposite coatings with low cytotoxicity and efficient antibacterial properties
Kena Ma,1,2,* Lingling Gong,1,* Xinjie Cai,1,2 Pin Huang,1 Jing Cai,1 Dan Huang,1 Tao Jiang1,2 1The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, 2Department of P...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2017-05-01
|
| Series: | International Journal of Nanomedicine |
| Subjects: | |
| Online Access: | https://www.dovepress.com/a-green-single-step-procedure-to-synthesize-ag-containing-nanocomposit-peer-reviewed-article-IJN |
| _version_ | 1818841077039235072 |
|---|---|
| author | Ma K Gong L Cai X Huang P Cai J Huang D Jiang T |
| author_facet | Ma K Gong L Cai X Huang P Cai J Huang D Jiang T |
| author_sort | Ma K |
| collection | DOAJ |
| description | Kena Ma,1,2,* Lingling Gong,1,* Xinjie Cai,1,2 Pin Huang,1 Jing Cai,1 Dan Huang,1 Tao Jiang1,2 1The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, 2Department of Prosthodontics, Hospital of Stomatology, Wuhan University, Wuhan, People’s Republic of China*These authors contributed equally to this workAbstract: Implant-associated infections still pose a serious threat leading to several complications. This study reported an environmentally benign Ag-containing nanocomposite coating with efficient antibacterial property fabricated on the metal implant via electrophoretic deposition (EPD). In such coatings, Ag2O/AgCl mixed with chitosan/gelatin (CS/G) polymers work together to exert the antibacterial property which could act as an alternative to traditional Ag nanoparticles. Scanning electron microscopy images showed the shuttle fiber-like morphology distributed lamellarly and some nanoparticles carved uniformly into the cross section. Transmission electron microscopy results revealed a core–shell-like structure of the released nanoparticles in experimental groups. The Ag-containing coatings exhibited strong antibacterial properties against Staphylococcus aureus strains and Escherichia coli strains. Meanwhile, the CCK-8 tests showed that after assembling with chitosan and gelatin polymers, the cytotoxicity of Ag was largely decreased. In addition, such coatings also exhibited strong bond strength with metal substrates and good degradable properties. Therefore, such Ag-containing CS/G coatings fabricated via EPD may be a promising candidate to be administrated in controlling the implant-associated infections.Keywords: infection, green chemistry, chitosan, gelatin, EPD |
| first_indexed | 2024-12-19T04:20:20Z |
| format | Article |
| id | doaj.art-d8c6548ad6fa4126b9530c84ac8f2600 |
| institution | Directory Open Access Journal |
| issn | 1178-2013 |
| language | English |
| last_indexed | 2024-12-19T04:20:20Z |
| publishDate | 2017-05-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-d8c6548ad6fa4126b9530c84ac8f26002022-12-21T20:36:10ZengDove Medical PressInternational Journal of Nanomedicine1178-20132017-05-01Volume 123665367932813A green single-step procedure to synthesize Ag-containing nanocomposite coatings with low cytotoxicity and efficient antibacterial propertiesMa KGong LCai XHuang PCai JHuang DJiang TKena Ma,1,2,* Lingling Gong,1,* Xinjie Cai,1,2 Pin Huang,1 Jing Cai,1 Dan Huang,1 Tao Jiang1,2 1The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, 2Department of Prosthodontics, Hospital of Stomatology, Wuhan University, Wuhan, People’s Republic of China*These authors contributed equally to this workAbstract: Implant-associated infections still pose a serious threat leading to several complications. This study reported an environmentally benign Ag-containing nanocomposite coating with efficient antibacterial property fabricated on the metal implant via electrophoretic deposition (EPD). In such coatings, Ag2O/AgCl mixed with chitosan/gelatin (CS/G) polymers work together to exert the antibacterial property which could act as an alternative to traditional Ag nanoparticles. Scanning electron microscopy images showed the shuttle fiber-like morphology distributed lamellarly and some nanoparticles carved uniformly into the cross section. Transmission electron microscopy results revealed a core–shell-like structure of the released nanoparticles in experimental groups. The Ag-containing coatings exhibited strong antibacterial properties against Staphylococcus aureus strains and Escherichia coli strains. Meanwhile, the CCK-8 tests showed that after assembling with chitosan and gelatin polymers, the cytotoxicity of Ag was largely decreased. In addition, such coatings also exhibited strong bond strength with metal substrates and good degradable properties. Therefore, such Ag-containing CS/G coatings fabricated via EPD may be a promising candidate to be administrated in controlling the implant-associated infections.Keywords: infection, green chemistry, chitosan, gelatin, EPDhttps://www.dovepress.com/a-green-single-step-procedure-to-synthesize-ag-containing-nanocomposit-peer-reviewed-article-IJNnanocomposite coatingssilverchitosangelatinEPD |
| spellingShingle | Ma K Gong L Cai X Huang P Cai J Huang D Jiang T A green single-step procedure to synthesize Ag-containing nanocomposite coatings with low cytotoxicity and efficient antibacterial properties International Journal of Nanomedicine nanocomposite coatings silver chitosan gelatin EPD |
| title | A green single-step procedure to synthesize Ag-containing nanocomposite coatings with low cytotoxicity and efficient antibacterial properties |
| title_full | A green single-step procedure to synthesize Ag-containing nanocomposite coatings with low cytotoxicity and efficient antibacterial properties |
| title_fullStr | A green single-step procedure to synthesize Ag-containing nanocomposite coatings with low cytotoxicity and efficient antibacterial properties |
| title_full_unstemmed | A green single-step procedure to synthesize Ag-containing nanocomposite coatings with low cytotoxicity and efficient antibacterial properties |
| title_short | A green single-step procedure to synthesize Ag-containing nanocomposite coatings with low cytotoxicity and efficient antibacterial properties |
| title_sort | green single step procedure to synthesize ag containing nanocomposite coatings with low cytotoxicity and efficient antibacterial properties |
| topic | nanocomposite coatings silver chitosan gelatin EPD |
| url | https://www.dovepress.com/a-green-single-step-procedure-to-synthesize-ag-containing-nanocomposit-peer-reviewed-article-IJN |
| work_keys_str_mv | AT mak agreensinglestepproceduretosynthesizeagcontainingnanocompositecoatingswithlowcytotoxicityandefficientantibacterialproperties AT gongl agreensinglestepproceduretosynthesizeagcontainingnanocompositecoatingswithlowcytotoxicityandefficientantibacterialproperties AT caix agreensinglestepproceduretosynthesizeagcontainingnanocompositecoatingswithlowcytotoxicityandefficientantibacterialproperties AT huangp agreensinglestepproceduretosynthesizeagcontainingnanocompositecoatingswithlowcytotoxicityandefficientantibacterialproperties AT caij agreensinglestepproceduretosynthesizeagcontainingnanocompositecoatingswithlowcytotoxicityandefficientantibacterialproperties AT huangd agreensinglestepproceduretosynthesizeagcontainingnanocompositecoatingswithlowcytotoxicityandefficientantibacterialproperties AT jiangt agreensinglestepproceduretosynthesizeagcontainingnanocompositecoatingswithlowcytotoxicityandefficientantibacterialproperties AT mak greensinglestepproceduretosynthesizeagcontainingnanocompositecoatingswithlowcytotoxicityandefficientantibacterialproperties AT gongl greensinglestepproceduretosynthesizeagcontainingnanocompositecoatingswithlowcytotoxicityandefficientantibacterialproperties AT caix greensinglestepproceduretosynthesizeagcontainingnanocompositecoatingswithlowcytotoxicityandefficientantibacterialproperties AT huangp greensinglestepproceduretosynthesizeagcontainingnanocompositecoatingswithlowcytotoxicityandefficientantibacterialproperties AT caij greensinglestepproceduretosynthesizeagcontainingnanocompositecoatingswithlowcytotoxicityandefficientantibacterialproperties AT huangd greensinglestepproceduretosynthesizeagcontainingnanocompositecoatingswithlowcytotoxicityandefficientantibacterialproperties AT jiangt greensinglestepproceduretosynthesizeagcontainingnanocompositecoatingswithlowcytotoxicityandefficientantibacterialproperties |