Antisense Peptide Technology for Diagnostic Tests and Bioengineering Research
Antisense peptide technology (APT) is based on a useful heuristic algorithm for rational peptide design. It was deduced from empirical observations that peptides consisting of complementary (sense and antisense) amino acids interact with higher probability and affinity than the randomly selected one...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/17/9106 |
| _version_ | 1797521404628828160 |
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| author | Nikola Štambuk Paško Konjevoda Josip Pavan |
| author_facet | Nikola Štambuk Paško Konjevoda Josip Pavan |
| author_sort | Nikola Štambuk |
| collection | DOAJ |
| description | Antisense peptide technology (APT) is based on a useful heuristic algorithm for rational peptide design. It was deduced from empirical observations that peptides consisting of complementary (sense and antisense) amino acids interact with higher probability and affinity than the randomly selected ones. This phenomenon is closely related to the structure of the standard genetic code table, and at the same time, is unrelated to the direction of its codon sequence translation. The concept of <i>complementary peptide interaction</i> is discussed, and its possible applications to diagnostic tests and bioengineering research are summarized. Problems and difficulties that may arise using APT are discussed, and possible solutions are proposed. The methodology was tested on the example of SARS-CoV-2. It is shown that the CABS-dock server accurately predicts the binding of antisense peptides to the SARS-CoV-2 receptor binding domain without requiring predefinition of the binding site. It is concluded that the benefits of APT outweigh the costs of random peptide screening and could lead to considerable savings in time and resources, especially if combined with other computational and immunochemical methods. |
| first_indexed | 2024-03-10T08:12:07Z |
| format | Article |
| id | doaj.art-d8c6ca34e6b740d78371b7a9496a9a2d |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T08:12:07Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-d8c6ca34e6b740d78371b7a9496a9a2d2023-11-22T10:38:10ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-08-012217910610.3390/ijms22179106Antisense Peptide Technology for Diagnostic Tests and Bioengineering ResearchNikola Štambuk0Paško Konjevoda1Josip Pavan2Center for Nuclear Magnetic Resonance, Ruđer Bošković Institute, Bijenička cesta 54, HR-10000 Zagreb, CroatiaLaboratory for Epigenomics, Division of Molecular Medicine, Ruđer Bošković Institute, Bijenička cesta 54, HR-10000 Zagreb, CroatiaDepartment of Ophthalmology, University Hospital Dubrava, Avenija Gojka Šuška 6, HR-10000 Zagreb, CroatiaAntisense peptide technology (APT) is based on a useful heuristic algorithm for rational peptide design. It was deduced from empirical observations that peptides consisting of complementary (sense and antisense) amino acids interact with higher probability and affinity than the randomly selected ones. This phenomenon is closely related to the structure of the standard genetic code table, and at the same time, is unrelated to the direction of its codon sequence translation. The concept of <i>complementary peptide interaction</i> is discussed, and its possible applications to diagnostic tests and bioengineering research are summarized. Problems and difficulties that may arise using APT are discussed, and possible solutions are proposed. The methodology was tested on the example of SARS-CoV-2. It is shown that the CABS-dock server accurately predicts the binding of antisense peptides to the SARS-CoV-2 receptor binding domain without requiring predefinition of the binding site. It is concluded that the benefits of APT outweigh the costs of random peptide screening and could lead to considerable savings in time and resources, especially if combined with other computational and immunochemical methods.https://www.mdpi.com/1422-0067/22/17/9106antisensecomplementarypeptidebindinggenetic codetechnology |
| spellingShingle | Nikola Štambuk Paško Konjevoda Josip Pavan Antisense Peptide Technology for Diagnostic Tests and Bioengineering Research International Journal of Molecular Sciences antisense complementary peptide binding genetic code technology |
| title | Antisense Peptide Technology for Diagnostic Tests and Bioengineering Research |
| title_full | Antisense Peptide Technology for Diagnostic Tests and Bioengineering Research |
| title_fullStr | Antisense Peptide Technology for Diagnostic Tests and Bioengineering Research |
| title_full_unstemmed | Antisense Peptide Technology for Diagnostic Tests and Bioengineering Research |
| title_short | Antisense Peptide Technology for Diagnostic Tests and Bioengineering Research |
| title_sort | antisense peptide technology for diagnostic tests and bioengineering research |
| topic | antisense complementary peptide binding genetic code technology |
| url | https://www.mdpi.com/1422-0067/22/17/9106 |
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