Influence of the extraction procedure on the antioxidative activity of lentil seed extracts in a β-carotene-linoleate model system
Phenolic compounds were extracted from lentil seeds using three solvent systems: 80% (v/v) acetone, 80% (v/v) methanol, and 80% (v/v) ethanol. Each extract was subsequently separated into two fractions by chromatoghraphy on a column with Toyo Pearl HW-40 using water (fraction I) and methanol (fracti...
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| Format: | Article |
| Language: | English |
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Consejo Superior de Investigaciones Científicas
2001-04-01
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| Series: | Grasas y Aceites |
| Subjects: | |
| Online Access: | http://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/378 |
| _version_ | 1818649678990802944 |
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| author | R. Amarowicz M. Karamac U. Chavan |
| author_facet | R. Amarowicz M. Karamac U. Chavan |
| author_sort | R. Amarowicz |
| collection | DOAJ |
| description | Phenolic compounds were extracted from lentil seeds using three solvent systems: 80% (v/v) acetone, 80% (v/v) methanol, and 80% (v/v) ethanol. Each extract was subsequently separated into two fractions by chromatoghraphy on a column with Toyo Pearl HW-40 using water (fraction I) and methanol (fraction II) for elution. Antioxidative activity of extracts and their respective fractions were examined in a β-carotene-linoleate model system. All three extracts exhibited similar antioxidant activity. Considering the level of phenolic compounds in extracts it seems that phenolic compounds from the acetone extract were less active than those from either the methanolic and ethanolic ones. Because the content of phenolics was about 16-fold lower in fraction I of the methanolic and ethanolic extracts compared to fraction II, the phenolics in fraction I of the methanol and ethanol extracts from lentil seeds are much more active than these in fraction II. A stronger antioxidant activity of fraction I from the acetone extract compared to the crude acetone extract was observed during the latter incubation stage. The reason was a relatively high level of phenolic compounds in this fraction. UV spectra confirmed that the phenolic compounds from the acetone extract were different compared to methanolic and ethanolic extracts. |
| first_indexed | 2024-12-17T01:38:09Z |
| format | Article |
| id | doaj.art-d8c7109c5a914dfdb20b61a45edc60c7 |
| institution | Directory Open Access Journal |
| issn | 0017-3495 1988-4214 |
| language | English |
| last_indexed | 2024-12-17T01:38:09Z |
| publishDate | 2001-04-01 |
| publisher | Consejo Superior de Investigaciones Científicas |
| record_format | Article |
| series | Grasas y Aceites |
| spelling | doaj.art-d8c7109c5a914dfdb20b61a45edc60c72022-12-21T22:08:24ZengConsejo Superior de Investigaciones CientíficasGrasas y Aceites0017-34951988-42142001-04-01522899310.3989/gya.2001.v52.i2.378375Influence of the extraction procedure on the antioxidative activity of lentil seed extracts in a β-carotene-linoleate model systemR. Amarowicz0M. Karamac1U. Chavan2Division of Food Science, Institute of Animal Reproduction and Food Research of Polish Academy of SciencesDivision of Food Science, Institute of Animal Reproduction and Food Research of Polish Academy of SciencesDepartment of Biochemistry, Mahatma Phule Agriculture UniversityPhenolic compounds were extracted from lentil seeds using three solvent systems: 80% (v/v) acetone, 80% (v/v) methanol, and 80% (v/v) ethanol. Each extract was subsequently separated into two fractions by chromatoghraphy on a column with Toyo Pearl HW-40 using water (fraction I) and methanol (fraction II) for elution. Antioxidative activity of extracts and their respective fractions were examined in a β-carotene-linoleate model system. All three extracts exhibited similar antioxidant activity. Considering the level of phenolic compounds in extracts it seems that phenolic compounds from the acetone extract were less active than those from either the methanolic and ethanolic ones. Because the content of phenolics was about 16-fold lower in fraction I of the methanolic and ethanolic extracts compared to fraction II, the phenolics in fraction I of the methanol and ethanol extracts from lentil seeds are much more active than these in fraction II. A stronger antioxidant activity of fraction I from the acetone extract compared to the crude acetone extract was observed during the latter incubation stage. The reason was a relatively high level of phenolic compounds in this fraction. UV spectra confirmed that the phenolic compounds from the acetone extract were different compared to methanolic and ethanolic extracts.http://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/378antioxidative activityβ-carotene linoleateextractionlentil seed (extract) |
| spellingShingle | R. Amarowicz M. Karamac U. Chavan Influence of the extraction procedure on the antioxidative activity of lentil seed extracts in a β-carotene-linoleate model system Grasas y Aceites antioxidative activity β-carotene linoleate extraction lentil seed (extract) |
| title | Influence of the extraction procedure on the antioxidative activity of lentil seed extracts in a β-carotene-linoleate model system |
| title_full | Influence of the extraction procedure on the antioxidative activity of lentil seed extracts in a β-carotene-linoleate model system |
| title_fullStr | Influence of the extraction procedure on the antioxidative activity of lentil seed extracts in a β-carotene-linoleate model system |
| title_full_unstemmed | Influence of the extraction procedure on the antioxidative activity of lentil seed extracts in a β-carotene-linoleate model system |
| title_short | Influence of the extraction procedure on the antioxidative activity of lentil seed extracts in a β-carotene-linoleate model system |
| title_sort | influence of the extraction procedure on the antioxidative activity of lentil seed extracts in a β carotene linoleate model system |
| topic | antioxidative activity β-carotene linoleate extraction lentil seed (extract) |
| url | http://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/378 |
| work_keys_str_mv | AT ramarowicz influenceoftheextractionprocedureontheantioxidativeactivityoflentilseedextractsinabcarotenelinoleatemodelsystem AT mkaramac influenceoftheextractionprocedureontheantioxidativeactivityoflentilseedextractsinabcarotenelinoleatemodelsystem AT uchavan influenceoftheextractionprocedureontheantioxidativeactivityoflentilseedextractsinabcarotenelinoleatemodelsystem |