Cyanobacterial neurotoxin BMAA and brain pathology in stranded dolphins.
Dolphin stranding events occur frequently in Florida and Massachusetts. Dolphins are an excellent sentinel species for toxin exposures in the marine environment. In this report we examine whether cyanobacterial neurotoxin, β-methylamino-L-alanine (BMAA), is present in stranded dolphins. BMAA has bee...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2019-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0213346 |
| _version_ | 1818361100196904960 |
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| author | David A Davis Kiyo Mondo Erica Stern Ama K Annor Susan J Murch Thomas M Coyne Larry E Brand Misty E Niemeyer Sarah Sharp Walter G Bradley Paul Alan Cox Deborah C Mash |
| author_facet | David A Davis Kiyo Mondo Erica Stern Ama K Annor Susan J Murch Thomas M Coyne Larry E Brand Misty E Niemeyer Sarah Sharp Walter G Bradley Paul Alan Cox Deborah C Mash |
| author_sort | David A Davis |
| collection | DOAJ |
| description | Dolphin stranding events occur frequently in Florida and Massachusetts. Dolphins are an excellent sentinel species for toxin exposures in the marine environment. In this report we examine whether cyanobacterial neurotoxin, β-methylamino-L-alanine (BMAA), is present in stranded dolphins. BMAA has been shown to bioaccumulate in the marine food web, including in the muscles and fins of sharks. Dietary exposure to BMAA is associated with the occurrence of neurofibrillary tangles and β-amyloid plaques in nonhuman primates. The findings of protein-bound BMAA in brain tissues from patients with Alzheimer's disease has advanced the hypothesis that BMAA may be linked to dementia. Since dolphins are apex predators and consume prey containing high amounts of BMAA, we examined necropsy specimens to determine if dietary and environmental exposures may result in the accumulation of BMAA in the brains of dolphins. To test this hypothesis, we measured BMAA in a series of brains collected from dolphins stranded in Florida and Massachusetts using two orthogonal analytical methods: 1) high performance liquid chromatography, and 2) ultra-performance liquid chromatography with tandem mass spectrometry. We detected high levels of BMAA (20-748 μg/g) in the brains of 13 of 14 dolphins. To correlate neuropathological changes with toxin exposure, gross and microscopic examinations were performed on cortical brain regions responsible for acoustico-motor navigation. We observed increased numbers of β-amyloid+ plaques and dystrophic neurites in the auditory cortex compared to the visual cortex and brainstem. The presence of BMAA and neuropathological changes in the stranded dolphin brain may help to further our understanding of cyanotoxin exposure and its potential impact on human health. |
| first_indexed | 2024-12-13T21:11:19Z |
| format | Article |
| id | doaj.art-d8caefdf1ee341f08d8b8832c464c514 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T21:11:19Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-d8caefdf1ee341f08d8b8832c464c5142022-12-21T23:31:21ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01143e021334610.1371/journal.pone.0213346Cyanobacterial neurotoxin BMAA and brain pathology in stranded dolphins.David A DavisKiyo MondoErica SternAma K AnnorSusan J MurchThomas M CoyneLarry E BrandMisty E NiemeyerSarah SharpWalter G BradleyPaul Alan CoxDeborah C MashDolphin stranding events occur frequently in Florida and Massachusetts. Dolphins are an excellent sentinel species for toxin exposures in the marine environment. In this report we examine whether cyanobacterial neurotoxin, β-methylamino-L-alanine (BMAA), is present in stranded dolphins. BMAA has been shown to bioaccumulate in the marine food web, including in the muscles and fins of sharks. Dietary exposure to BMAA is associated with the occurrence of neurofibrillary tangles and β-amyloid plaques in nonhuman primates. The findings of protein-bound BMAA in brain tissues from patients with Alzheimer's disease has advanced the hypothesis that BMAA may be linked to dementia. Since dolphins are apex predators and consume prey containing high amounts of BMAA, we examined necropsy specimens to determine if dietary and environmental exposures may result in the accumulation of BMAA in the brains of dolphins. To test this hypothesis, we measured BMAA in a series of brains collected from dolphins stranded in Florida and Massachusetts using two orthogonal analytical methods: 1) high performance liquid chromatography, and 2) ultra-performance liquid chromatography with tandem mass spectrometry. We detected high levels of BMAA (20-748 μg/g) in the brains of 13 of 14 dolphins. To correlate neuropathological changes with toxin exposure, gross and microscopic examinations were performed on cortical brain regions responsible for acoustico-motor navigation. We observed increased numbers of β-amyloid+ plaques and dystrophic neurites in the auditory cortex compared to the visual cortex and brainstem. The presence of BMAA and neuropathological changes in the stranded dolphin brain may help to further our understanding of cyanotoxin exposure and its potential impact on human health.https://doi.org/10.1371/journal.pone.0213346 |
| spellingShingle | David A Davis Kiyo Mondo Erica Stern Ama K Annor Susan J Murch Thomas M Coyne Larry E Brand Misty E Niemeyer Sarah Sharp Walter G Bradley Paul Alan Cox Deborah C Mash Cyanobacterial neurotoxin BMAA and brain pathology in stranded dolphins. PLoS ONE |
| title | Cyanobacterial neurotoxin BMAA and brain pathology in stranded dolphins. |
| title_full | Cyanobacterial neurotoxin BMAA and brain pathology in stranded dolphins. |
| title_fullStr | Cyanobacterial neurotoxin BMAA and brain pathology in stranded dolphins. |
| title_full_unstemmed | Cyanobacterial neurotoxin BMAA and brain pathology in stranded dolphins. |
| title_short | Cyanobacterial neurotoxin BMAA and brain pathology in stranded dolphins. |
| title_sort | cyanobacterial neurotoxin bmaa and brain pathology in stranded dolphins |
| url | https://doi.org/10.1371/journal.pone.0213346 |
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