Frequency of BCR-ABL Fusion Transcript in Iranian Patients with Chronic Myeloid Leukemia
Introduction: Reverse transcriptase-polymerase chain reaction (RT-PCR) assay is a useful tool for the detection of fusion transcript resulting from specific chromosomal translocation of the leukemia cells. A specific chromosomal abnormality, the Philadelphia chromosome (Ph), is present in 90% to 95%...
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Tehran University of Medical Sciences
2005-09-01
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| Series: | International Journal of Hematology-Oncology and Stem Cell Research |
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| Online Access: | http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/3197.pdf&manuscript_id=3197 |
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| author | Yaghmaie M. Ghaffari S.H. Alimoghaddam K. Ghavamzadeh A. Mousavi S.A. Irvani M. Bahar B. Bibordi E. Jahani M |
| author_facet | Yaghmaie M. Ghaffari S.H. Alimoghaddam K. Ghavamzadeh A. Mousavi S.A. Irvani M. Bahar B. Bibordi E. Jahani M |
| author_sort | Yaghmaie M. |
| collection | DOAJ |
| description | Introduction: Reverse transcriptase-polymerase chain reaction (RT-PCR) assay is a useful tool for the detection of fusion transcript resulting from specific chromosomal translocation of the leukemia cells. A specific chromosomal abnormality, the Philadelphia chromosome (Ph), is present in 90% to 95% of CML patients.The aberration results from a reciprocal translocation between chromosome 9 and 22, creating a BCR-ABL fusion gene.There are two major forms of the BCR/ABL fusion gene, involving ABL exon 2, but including different exons of BCR gene. The transcripts b2a2 or b3a2 code for a p210 protein. Another fusion gene leads to the expression of an e1a2 transcript, which codes for a p190 pro-tein. Another, less common fusion genes are b3a3 or b2a3 (p203) and e19a2 (p230). The incidence of one or other rearrangement in chronic myeloid leukemia (CML) patients varies in different reported se-ries. In general, fusion transcripts are determined individually, a process which is labor intensive in or-der to detect all major fusion transcripts. Methods: This study was designed to determine the frequency of different fusion genes in 75 iranian patients with CML. peripheral blood samples were analyzed by multiplex reverse transcriptase poly-merase chain reaction (RT-PCR) from adult patients to detect all types of BCR-ABL transcripts of the t (9:22) and found that all cases were positive for some type of BCR/ABL rearrangement. Results: Most of our patients showed b3a2 fusion gene (62%), while the remaining showed one of the transcripts of b2a2, b3a3, b2a3, e1a2 or coexpression of b3a2 and b2a2. The rate of coexpression of the b3a2 and b2a2 was 5%. Conclusion: In contrast to the other reports, we did not see any coexpression of p210/p190. This may reflect either the sensitivity of the detection techniques used or the possibility of genetic differences be-tween the populations studied. Coexpression may be due to alternative splicing or to phenotypic varia-tion, with clinical course different from classical CML. |
| first_indexed | 2024-03-12T09:02:10Z |
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| issn | 2008-3009 2008-2207 |
| language | English |
| last_indexed | 2024-03-12T09:02:10Z |
| publishDate | 2005-09-01 |
| publisher | Tehran University of Medical Sciences |
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| series | International Journal of Hematology-Oncology and Stem Cell Research |
| spelling | doaj.art-d8cdad1e2afb4f0f92f0de449cadcbae2023-09-02T15:37:08ZengTehran University of Medical SciencesInternational Journal of Hematology-Oncology and Stem Cell Research2008-30092008-22072005-09-012315Frequency of BCR-ABL Fusion Transcript in Iranian Patients with Chronic Myeloid LeukemiaYaghmaie M.Ghaffari S.H.Alimoghaddam K.Ghavamzadeh A.Mousavi S.A.Irvani M.Bahar B.Bibordi E.Jahani MIntroduction: Reverse transcriptase-polymerase chain reaction (RT-PCR) assay is a useful tool for the detection of fusion transcript resulting from specific chromosomal translocation of the leukemia cells. A specific chromosomal abnormality, the Philadelphia chromosome (Ph), is present in 90% to 95% of CML patients.The aberration results from a reciprocal translocation between chromosome 9 and 22, creating a BCR-ABL fusion gene.There are two major forms of the BCR/ABL fusion gene, involving ABL exon 2, but including different exons of BCR gene. The transcripts b2a2 or b3a2 code for a p210 protein. Another fusion gene leads to the expression of an e1a2 transcript, which codes for a p190 pro-tein. Another, less common fusion genes are b3a3 or b2a3 (p203) and e19a2 (p230). The incidence of one or other rearrangement in chronic myeloid leukemia (CML) patients varies in different reported se-ries. In general, fusion transcripts are determined individually, a process which is labor intensive in or-der to detect all major fusion transcripts. Methods: This study was designed to determine the frequency of different fusion genes in 75 iranian patients with CML. peripheral blood samples were analyzed by multiplex reverse transcriptase poly-merase chain reaction (RT-PCR) from adult patients to detect all types of BCR-ABL transcripts of the t (9:22) and found that all cases were positive for some type of BCR/ABL rearrangement. Results: Most of our patients showed b3a2 fusion gene (62%), while the remaining showed one of the transcripts of b2a2, b3a3, b2a3, e1a2 or coexpression of b3a2 and b2a2. The rate of coexpression of the b3a2 and b2a2 was 5%. Conclusion: In contrast to the other reports, we did not see any coexpression of p210/p190. This may reflect either the sensitivity of the detection techniques used or the possibility of genetic differences be-tween the populations studied. Coexpression may be due to alternative splicing or to phenotypic varia-tion, with clinical course different from classical CML.http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/3197.pdf&manuscript_id=3197 BCR-ABLCMLMultiplex RT-PC |
| spellingShingle | Yaghmaie M. Ghaffari S.H. Alimoghaddam K. Ghavamzadeh A. Mousavi S.A. Irvani M. Bahar B. Bibordi E. Jahani M Frequency of BCR-ABL Fusion Transcript in Iranian Patients with Chronic Myeloid Leukemia International Journal of Hematology-Oncology and Stem Cell Research BCR-ABL CML Multiplex RT-PC |
| title | Frequency of BCR-ABL Fusion Transcript in Iranian Patients with Chronic Myeloid Leukemia |
| title_full | Frequency of BCR-ABL Fusion Transcript in Iranian Patients with Chronic Myeloid Leukemia |
| title_fullStr | Frequency of BCR-ABL Fusion Transcript in Iranian Patients with Chronic Myeloid Leukemia |
| title_full_unstemmed | Frequency of BCR-ABL Fusion Transcript in Iranian Patients with Chronic Myeloid Leukemia |
| title_short | Frequency of BCR-ABL Fusion Transcript in Iranian Patients with Chronic Myeloid Leukemia |
| title_sort | frequency of bcr abl fusion transcript in iranian patients with chronic myeloid leukemia |
| topic | BCR-ABL CML Multiplex RT-PC |
| url | http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/3197.pdf&manuscript_id=3197 |
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