Persicaline, an alkaloid from Salvadora persica, inhibits proliferation and induces apoptosis and cell-cycle arrest in MCF-7 cells
Cancer is the second largest cause of mortality worldwide. Many natural bioactive chemicals generated from plants have favorable impacts on health, including cancer chemoprevention, compared to their manufactured counterparts. Persicaline, a novel sulfur-containing imidazoline alkaloid derived from...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
2023-03-01
|
| Series: | Open Chemistry |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/chem-2022-0302 |
| _version_ | 1797848734984306688 |
|---|---|
| author | Mohany Mohamed Al-zharani Mohammed Nasr Fahd A. El-Wetidy Mohammad S. Farag Mohamed Abdel-Mageed Wael M. El-Gamal Ali Al-Rejaie Salim S. Noman Omar M. Qurtam Ashraf Ahmed Rudayni Hassan A. Aleissa Mohammed S. |
| author_facet | Mohany Mohamed Al-zharani Mohammed Nasr Fahd A. El-Wetidy Mohammad S. Farag Mohamed Abdel-Mageed Wael M. El-Gamal Ali Al-Rejaie Salim S. Noman Omar M. Qurtam Ashraf Ahmed Rudayni Hassan A. Aleissa Mohammed S. |
| author_sort | Mohany Mohamed |
| collection | DOAJ |
| description | Cancer is the second largest cause of mortality worldwide. Many natural bioactive chemicals generated from plants have favorable impacts on health, including cancer chemoprevention, compared to their manufactured counterparts. Persicaline, a novel sulfur-containing imidazoline alkaloid derived from Salvadora persica, has been shown to display promising antioxidant activity. In this study, the antiproliferative activity of persicaline was tested against different cancer cells using (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay. The cell death mode and cell-cycle arrest were examined using flow cytometry analysis. In addition, the proapoptotic and molecular mechanism effects of persicaline against mammary MCF-7 cell line were explored. Furthermore, the impact of persicaline on apoptotic genes markers, generation of reactive oxygen species (ROS), and mitochondrial membrane potential were monitored. It was found that persicaline inhibits cell proliferation in a dose-dependent manner. Persicaline-treated MCF-7 cells also showed initiation of apoptotic events and G1 cell-cycle arrest. In addition, persicaline treatment led to an increase in ROS generation, Bax and caspase upregulation while the Bcl-2 was downregulated. Hence, for the first time, this study showed that persicaline causes G1 phase arrest and apoptosis induction in MCF-7 cells. Increased proapoptotic genes and ROS levels were required for the antiproliferative and apoptotic effects of persicaline. |
| first_indexed | 2024-04-09T18:32:22Z |
| format | Article |
| id | doaj.art-d8cf04454c164cc0a578faea5b292a41 |
| institution | Directory Open Access Journal |
| issn | 2391-5420 |
| language | English |
| last_indexed | 2024-04-09T18:32:22Z |
| publishDate | 2023-03-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Chemistry |
| spelling | doaj.art-d8cf04454c164cc0a578faea5b292a412023-04-11T17:07:15ZengDe GruyterOpen Chemistry2391-54202023-03-01211209711610.1515/chem-2022-0302Persicaline, an alkaloid from Salvadora persica, inhibits proliferation and induces apoptosis and cell-cycle arrest in MCF-7 cellsMohany Mohamed0Al-zharani Mohammed1Nasr Fahd A.2El-Wetidy Mohammad S.3Farag Mohamed4Abdel-Mageed Wael M.5El-Gamal Ali6Al-Rejaie Salim S.7Noman Omar M.8Qurtam Ashraf Ahmed9Rudayni Hassan A.10Aleissa Mohammed S.11Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, P.O. Box 55760, Riyadh – 1145, Saudi ArabiaBiology Department, College of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh 11623, Saudi ArabiaDepartment of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi ArabiaCollege of Medicine, Research Center, King Saud University, P.O. Box 2925, Riyadh 11451, Saudi ArabiaDepartment of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi ArabiaDepartment of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi ArabiaDepartment of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi ArabiaDepartment of Pharmacology and Toxicology, College of Pharmacy, King Saud University, P.O. Box 55760, Riyadh – 1145, Saudi ArabiaDepartment of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi ArabiaBiology Department, College of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh 11623, Saudi ArabiaBiology Department, College of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh 11623, Saudi ArabiaBiology Department, College of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh 11623, Saudi ArabiaCancer is the second largest cause of mortality worldwide. Many natural bioactive chemicals generated from plants have favorable impacts on health, including cancer chemoprevention, compared to their manufactured counterparts. Persicaline, a novel sulfur-containing imidazoline alkaloid derived from Salvadora persica, has been shown to display promising antioxidant activity. In this study, the antiproliferative activity of persicaline was tested against different cancer cells using (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay. The cell death mode and cell-cycle arrest were examined using flow cytometry analysis. In addition, the proapoptotic and molecular mechanism effects of persicaline against mammary MCF-7 cell line were explored. Furthermore, the impact of persicaline on apoptotic genes markers, generation of reactive oxygen species (ROS), and mitochondrial membrane potential were monitored. It was found that persicaline inhibits cell proliferation in a dose-dependent manner. Persicaline-treated MCF-7 cells also showed initiation of apoptotic events and G1 cell-cycle arrest. In addition, persicaline treatment led to an increase in ROS generation, Bax and caspase upregulation while the Bcl-2 was downregulated. Hence, for the first time, this study showed that persicaline causes G1 phase arrest and apoptosis induction in MCF-7 cells. Increased proapoptotic genes and ROS levels were required for the antiproliferative and apoptotic effects of persicaline.https://doi.org/10.1515/chem-2022-0302apoptosissalvadora persicareactive oxygen speciescell cycle |
| spellingShingle | Mohany Mohamed Al-zharani Mohammed Nasr Fahd A. El-Wetidy Mohammad S. Farag Mohamed Abdel-Mageed Wael M. El-Gamal Ali Al-Rejaie Salim S. Noman Omar M. Qurtam Ashraf Ahmed Rudayni Hassan A. Aleissa Mohammed S. Persicaline, an alkaloid from Salvadora persica, inhibits proliferation and induces apoptosis and cell-cycle arrest in MCF-7 cells Open Chemistry apoptosis salvadora persica reactive oxygen species cell cycle |
| title | Persicaline, an alkaloid from Salvadora persica, inhibits proliferation and induces apoptosis and cell-cycle arrest in MCF-7 cells |
| title_full | Persicaline, an alkaloid from Salvadora persica, inhibits proliferation and induces apoptosis and cell-cycle arrest in MCF-7 cells |
| title_fullStr | Persicaline, an alkaloid from Salvadora persica, inhibits proliferation and induces apoptosis and cell-cycle arrest in MCF-7 cells |
| title_full_unstemmed | Persicaline, an alkaloid from Salvadora persica, inhibits proliferation and induces apoptosis and cell-cycle arrest in MCF-7 cells |
| title_short | Persicaline, an alkaloid from Salvadora persica, inhibits proliferation and induces apoptosis and cell-cycle arrest in MCF-7 cells |
| title_sort | persicaline an alkaloid from salvadora persica inhibits proliferation and induces apoptosis and cell cycle arrest in mcf 7 cells |
| topic | apoptosis salvadora persica reactive oxygen species cell cycle |
| url | https://doi.org/10.1515/chem-2022-0302 |
| work_keys_str_mv | AT mohanymohamed persicalineanalkaloidfromsalvadorapersicainhibitsproliferationandinducesapoptosisandcellcyclearrestinmcf7cells AT alzharanimohammed persicalineanalkaloidfromsalvadorapersicainhibitsproliferationandinducesapoptosisandcellcyclearrestinmcf7cells AT nasrfahda persicalineanalkaloidfromsalvadorapersicainhibitsproliferationandinducesapoptosisandcellcyclearrestinmcf7cells AT elwetidymohammads persicalineanalkaloidfromsalvadorapersicainhibitsproliferationandinducesapoptosisandcellcyclearrestinmcf7cells AT faragmohamed persicalineanalkaloidfromsalvadorapersicainhibitsproliferationandinducesapoptosisandcellcyclearrestinmcf7cells AT abdelmageedwaelm persicalineanalkaloidfromsalvadorapersicainhibitsproliferationandinducesapoptosisandcellcyclearrestinmcf7cells AT elgamalali persicalineanalkaloidfromsalvadorapersicainhibitsproliferationandinducesapoptosisandcellcyclearrestinmcf7cells AT alrejaiesalims persicalineanalkaloidfromsalvadorapersicainhibitsproliferationandinducesapoptosisandcellcyclearrestinmcf7cells AT nomanomarm persicalineanalkaloidfromsalvadorapersicainhibitsproliferationandinducesapoptosisandcellcyclearrestinmcf7cells AT qurtamashrafahmed persicalineanalkaloidfromsalvadorapersicainhibitsproliferationandinducesapoptosisandcellcyclearrestinmcf7cells AT rudaynihassana persicalineanalkaloidfromsalvadorapersicainhibitsproliferationandinducesapoptosisandcellcyclearrestinmcf7cells AT aleissamohammeds persicalineanalkaloidfromsalvadorapersicainhibitsproliferationandinducesapoptosisandcellcyclearrestinmcf7cells |