Seasonal Occurrence and Carbapenem Susceptibility of Bovine Acinetobacter baumannii in Germany
Acinetobacter baumannii is one of the leading causes of nosocomial infections in humans. To investigate its prevalence, distribution of sequence types (STs), and antimicrobial resistance in cattle, we sampled 422 cattle, including 280 dairy cows, 59 beef cattle, and 83 calves over a 14-month period....
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.00272/full |
| _version_ | 1828855769708101632 |
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| author | Peter Klotz Paul G. Higgins Paul G. Higgins Andreas R. Schaubmar Klaus Failing Ursula Leidner Harald Seifert Harald Seifert Sandra Scheufen Sandra Scheufen Torsten Semmler Christa Ewers |
| author_facet | Peter Klotz Paul G. Higgins Paul G. Higgins Andreas R. Schaubmar Klaus Failing Ursula Leidner Harald Seifert Harald Seifert Sandra Scheufen Sandra Scheufen Torsten Semmler Christa Ewers |
| author_sort | Peter Klotz |
| collection | DOAJ |
| description | Acinetobacter baumannii is one of the leading causes of nosocomial infections in humans. To investigate its prevalence, distribution of sequence types (STs), and antimicrobial resistance in cattle, we sampled 422 cattle, including 280 dairy cows, 59 beef cattle, and 83 calves over a 14-month period. Metadata, such as the previous use of antimicrobial agents and feeding, were collected to identify putative determining factors. Bacterial isolates were identified via MALDI-TOF/MS and PCR, antimicrobial susceptibility was evaluated via VITEK2 and antibiotic gradient tests, resistance genes were identified by PCR. Overall, 15.6% of the cattle harbored A. baumannii, predominantly in the nose (60.3% of the A. baumannii isolates). It was more frequent in dairy cows (21.1%) than in beef cattle (6.8%) and calves (2.4%). A seasonal occurrence was shown with a peak between May and August. The rate of occurrence of A. baumannii was correlated with a history of use of 3rd generation cephalosporins in the last 6 months prior to sampling Multilocus sequence typing (Pasteur scheme) revealed 83 STs among 126 unique isolates. Nine of the bovine STs have previously been implicated in human infections. Besides known intrinsic resistance of the species, the isolates did not show additional resistance to the antimicrobial substances tested, including carbapenems. Our data suggest that cattle are not a reservoir for nosocomial A. baumannii but carry a highly diverse population of this species. Nevertheless, some STs seem to be able to colonize both cattle and humans. |
| first_indexed | 2024-12-13T01:00:51Z |
| format | Article |
| id | doaj.art-d8d0932cd64a44fb96fe4f7ac630d5ec |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-13T01:00:51Z |
| publishDate | 2019-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-d8d0932cd64a44fb96fe4f7ac630d5ec2022-12-22T00:04:41ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-02-011010.3389/fmicb.2019.00272441436Seasonal Occurrence and Carbapenem Susceptibility of Bovine Acinetobacter baumannii in GermanyPeter Klotz0Paul G. Higgins1Paul G. Higgins2Andreas R. Schaubmar3Klaus Failing4Ursula Leidner5Harald Seifert6Harald Seifert7Sandra Scheufen8Sandra Scheufen9Torsten Semmler10Christa Ewers11Institute of Hygiene and Infectious Diseases of Animals, Justus Liebig University Giessen, Giessen, GermanyInstitute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Cologne, GermanyGerman Center for Infection Research (DZIF), Partner Site Bonn-Cologne, Cologne, GermanyUnit for Biomathematics and Data Processing, Faculty of Veterinary Medicine, Justus Liebig University Giessen, Giessen, GermanyUnit for Biomathematics and Data Processing, Faculty of Veterinary Medicine, Justus Liebig University Giessen, Giessen, GermanyInstitute of Hygiene and Infectious Diseases of Animals, Justus Liebig University Giessen, Giessen, GermanyInstitute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Cologne, GermanyGerman Center for Infection Research (DZIF), Partner Site Bonn-Cologne, Cologne, GermanyInstitute of Hygiene and Infectious Diseases of Animals, Justus Liebig University Giessen, Giessen, GermanyInstitute for Hygiene and Microbiology, University of Würzburg, Würzburg, GermanyNG1 Microbial Genomics, Robert Koch Institute, Berlin, GermanyInstitute of Hygiene and Infectious Diseases of Animals, Justus Liebig University Giessen, Giessen, GermanyAcinetobacter baumannii is one of the leading causes of nosocomial infections in humans. To investigate its prevalence, distribution of sequence types (STs), and antimicrobial resistance in cattle, we sampled 422 cattle, including 280 dairy cows, 59 beef cattle, and 83 calves over a 14-month period. Metadata, such as the previous use of antimicrobial agents and feeding, were collected to identify putative determining factors. Bacterial isolates were identified via MALDI-TOF/MS and PCR, antimicrobial susceptibility was evaluated via VITEK2 and antibiotic gradient tests, resistance genes were identified by PCR. Overall, 15.6% of the cattle harbored A. baumannii, predominantly in the nose (60.3% of the A. baumannii isolates). It was more frequent in dairy cows (21.1%) than in beef cattle (6.8%) and calves (2.4%). A seasonal occurrence was shown with a peak between May and August. The rate of occurrence of A. baumannii was correlated with a history of use of 3rd generation cephalosporins in the last 6 months prior to sampling Multilocus sequence typing (Pasteur scheme) revealed 83 STs among 126 unique isolates. Nine of the bovine STs have previously been implicated in human infections. Besides known intrinsic resistance of the species, the isolates did not show additional resistance to the antimicrobial substances tested, including carbapenems. Our data suggest that cattle are not a reservoir for nosocomial A. baumannii but carry a highly diverse population of this species. Nevertheless, some STs seem to be able to colonize both cattle and humans.https://www.frontiersin.org/article/10.3389/fmicb.2019.00272/fullESKAPEAcinetobacter baumanniiantimicrobial susceptibilityMLSTcattleepidemiology |
| spellingShingle | Peter Klotz Paul G. Higgins Paul G. Higgins Andreas R. Schaubmar Klaus Failing Ursula Leidner Harald Seifert Harald Seifert Sandra Scheufen Sandra Scheufen Torsten Semmler Christa Ewers Seasonal Occurrence and Carbapenem Susceptibility of Bovine Acinetobacter baumannii in Germany Frontiers in Microbiology ESKAPE Acinetobacter baumannii antimicrobial susceptibility MLST cattle epidemiology |
| title | Seasonal Occurrence and Carbapenem Susceptibility of Bovine Acinetobacter baumannii in Germany |
| title_full | Seasonal Occurrence and Carbapenem Susceptibility of Bovine Acinetobacter baumannii in Germany |
| title_fullStr | Seasonal Occurrence and Carbapenem Susceptibility of Bovine Acinetobacter baumannii in Germany |
| title_full_unstemmed | Seasonal Occurrence and Carbapenem Susceptibility of Bovine Acinetobacter baumannii in Germany |
| title_short | Seasonal Occurrence and Carbapenem Susceptibility of Bovine Acinetobacter baumannii in Germany |
| title_sort | seasonal occurrence and carbapenem susceptibility of bovine acinetobacter baumannii in germany |
| topic | ESKAPE Acinetobacter baumannii antimicrobial susceptibility MLST cattle epidemiology |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.00272/full |
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