Usefulness of COL11A1 as a Prognostic Marker of Tumor Infiltration
Background: Determining the infiltration of carcinomas is essential for the proper follow-up and treatment of cancer patients. However, it continues to be a diagnostic challenge for pathologists in multiple types of tumors. In previous studies (carried out in surgical specimens), the protein COL11A1...
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MDPI AG
2023-09-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/11/9/2496 |
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| author | Javier Freire Pilar García-Berbel Belén Caramelo Lucía García-Berbel Victor J. Ovejero Nuria Cadenas Ainara Azueta Javier Gómez-Román |
| author_facet | Javier Freire Pilar García-Berbel Belén Caramelo Lucía García-Berbel Victor J. Ovejero Nuria Cadenas Ainara Azueta Javier Gómez-Román |
| author_sort | Javier Freire |
| collection | DOAJ |
| description | Background: Determining the infiltration of carcinomas is essential for the proper follow-up and treatment of cancer patients. However, it continues to be a diagnostic challenge for pathologists in multiple types of tumors. In previous studies (carried out in surgical specimens), the protein COL11A1 has been postulated as an infiltration marker mainly expressed in the extracellular matrix (ECM). We hypothesized that a differential expression of COL11A1 may exist in the peritumoral stroma of tumors that have acquired infiltrating properties and that it may be detected in the small biopsies usually available in normal clinical practice. Material and methods: In our study, we performed immunohistochemical staining in more than 350 invasive and noninvasive small samples obtained via core needle biopsy (CNB), colonoscopy, or transurethral resection of bladder tumor (TURBT) of breast, colorectal, bladder, and ovarian cancer. Results: Our results revealed that COL11A1 immunostaining had a sensitivity to classify the samples into infiltrative vs. noninfiltrative tumors of 94% (breast), 97% (colorectal), >90% (bladder), and 74% (ovarian); and a specificity of 97% (breast), 100% (colorectal), and >90% (bladder). In ovarian cancer, the negative predictive value (0.59) did not present improvement over the usual histopathological markers. In all samples tested, the cumulative sensitivity was 86% and the specificity 96% (<i>p</i> < 0.0001). Conclusions: COL11A1-positive immunostaining in small biopsies of breast, colon, bladder and ovarian cancer is an accurate predictive marker of tumor infiltration that can be easily implemented in daily clinical practice. |
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| language | English |
| last_indexed | 2024-03-10T23:00:47Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-d8d20c966cfd4baebaae204a6f4ded6a2023-11-19T09:42:09ZengMDPI AGBiomedicines2227-90592023-09-01119249610.3390/biomedicines11092496Usefulness of COL11A1 as a Prognostic Marker of Tumor InfiltrationJavier Freire0Pilar García-Berbel1Belén Caramelo2Lucía García-Berbel3Victor J. Ovejero4Nuria Cadenas5Ainara Azueta6Javier Gómez-Román7Pathology Department, University Hospital Marques de Valdecilla, Avda. Marqués de Valdecilla s/n, 39008 Santander, SpainPathology and Molecular Pathology Unit, IDIVAL, Avenida Cardenal Herrera Oria s/n, 39011 Santander, SpainPathology and Molecular Pathology Unit, IDIVAL, Avenida Cardenal Herrera Oria s/n, 39011 Santander, SpainBreast Unit, Gynecology Department, University Hospital Puerta del Mar. Av. Ana de Viya, 21, 11009 Cádiz, SpainSurgery Department, University Hospital Marques de Valdecilla, Avda. Marqués de Valdecilla s/n, 39008 Santander, SpainEl Alisal Health Center, Cantabrian Health Service, C. los Ciruelos, 48, 39011 Santander, SpainPathology Department, University Hospital Marques de Valdecilla, Avda. Marqués de Valdecilla s/n, 39008 Santander, SpainPathology Department, University Hospital Marques de Valdecilla, Avda. Marqués de Valdecilla s/n, 39008 Santander, SpainBackground: Determining the infiltration of carcinomas is essential for the proper follow-up and treatment of cancer patients. However, it continues to be a diagnostic challenge for pathologists in multiple types of tumors. In previous studies (carried out in surgical specimens), the protein COL11A1 has been postulated as an infiltration marker mainly expressed in the extracellular matrix (ECM). We hypothesized that a differential expression of COL11A1 may exist in the peritumoral stroma of tumors that have acquired infiltrating properties and that it may be detected in the small biopsies usually available in normal clinical practice. Material and methods: In our study, we performed immunohistochemical staining in more than 350 invasive and noninvasive small samples obtained via core needle biopsy (CNB), colonoscopy, or transurethral resection of bladder tumor (TURBT) of breast, colorectal, bladder, and ovarian cancer. Results: Our results revealed that COL11A1 immunostaining had a sensitivity to classify the samples into infiltrative vs. noninfiltrative tumors of 94% (breast), 97% (colorectal), >90% (bladder), and 74% (ovarian); and a specificity of 97% (breast), 100% (colorectal), and >90% (bladder). In ovarian cancer, the negative predictive value (0.59) did not present improvement over the usual histopathological markers. In all samples tested, the cumulative sensitivity was 86% and the specificity 96% (<i>p</i> < 0.0001). Conclusions: COL11A1-positive immunostaining in small biopsies of breast, colon, bladder and ovarian cancer is an accurate predictive marker of tumor infiltration that can be easily implemented in daily clinical practice.https://www.mdpi.com/2227-9059/11/9/2496COL11A1collagen XI alpha 1tumor metastasistumor activated fibroblast |
| spellingShingle | Javier Freire Pilar García-Berbel Belén Caramelo Lucía García-Berbel Victor J. Ovejero Nuria Cadenas Ainara Azueta Javier Gómez-Román Usefulness of COL11A1 as a Prognostic Marker of Tumor Infiltration Biomedicines COL11A1 collagen XI alpha 1 tumor metastasis tumor activated fibroblast |
| title | Usefulness of COL11A1 as a Prognostic Marker of Tumor Infiltration |
| title_full | Usefulness of COL11A1 as a Prognostic Marker of Tumor Infiltration |
| title_fullStr | Usefulness of COL11A1 as a Prognostic Marker of Tumor Infiltration |
| title_full_unstemmed | Usefulness of COL11A1 as a Prognostic Marker of Tumor Infiltration |
| title_short | Usefulness of COL11A1 as a Prognostic Marker of Tumor Infiltration |
| title_sort | usefulness of col11a1 as a prognostic marker of tumor infiltration |
| topic | COL11A1 collagen XI alpha 1 tumor metastasis tumor activated fibroblast |
| url | https://www.mdpi.com/2227-9059/11/9/2496 |
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