Low-Cost High-Resolution Potentiostat for Electrochemical Detection of Nucleic Acids and Biomolecular Interactions
A handheld USB-powered instrument developed for the electrochemical detection of nucleic acids and biomolecular interactions is presented. The proposed instrument is capable of scanning ± 2.25 V while measuring currents up to ±10 mA, with a minimum current resolution of 6.87 pA. Therefore, it is sui...
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MDPI AG
2022-09-01
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Online Access: | https://www.mdpi.com/2072-666X/13/10/1610 |
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author | Alper Demirhan Ece Eksin Yalin Kilic Arzum Erdem |
author_facet | Alper Demirhan Ece Eksin Yalin Kilic Arzum Erdem |
author_sort | Alper Demirhan |
collection | DOAJ |
description | A handheld USB-powered instrument developed for the electrochemical detection of nucleic acids and biomolecular interactions is presented. The proposed instrument is capable of scanning ± 2.25 V while measuring currents up to ±10 mA, with a minimum current resolution of 6.87 pA. Therefore, it is suitable for nucleic acid sensors, which have high background currents. A low-cost microcontroller with an on-chip 16-bit analog-to-digital converter, 12-bit digital-to-analog converter, and a built-in USB controller were used to miniaturize the system. The offset voltages and gain errors of the analog peripherals were calibrated to obtain a superior performance. Thus, a similar performance to those of the market-leader potentiostats was achieved, but at a fraction of their cost and size. The performance of the application of this proposed architecture was tested successfully and was found to be similar to a leading commercial device through a clinical application in the aspects of the detection of nucleic acids, such as calf thymus ssDNA and dsDNA, and their interactions with a protein (BSA) by using single-use graphite electrodes in combination with the differential pulse voltammetry technique. |
first_indexed | 2024-03-09T19:47:42Z |
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id | doaj.art-d8d2c78a270e44ae9b9acaef076d76d0 |
institution | Directory Open Access Journal |
issn | 2072-666X |
language | English |
last_indexed | 2024-03-09T19:47:42Z |
publishDate | 2022-09-01 |
publisher | MDPI AG |
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series | Micromachines |
spelling | doaj.art-d8d2c78a270e44ae9b9acaef076d76d02023-11-24T01:21:28ZengMDPI AGMicromachines2072-666X2022-09-011310161010.3390/mi13101610Low-Cost High-Resolution Potentiostat for Electrochemical Detection of Nucleic Acids and Biomolecular InteractionsAlper Demirhan0Ece Eksin1Yalin Kilic2Arzum Erdem3Department of Biotechnology, Graduate School of Natural and Applied Sciences, Ege University, Izmir 35100, TurkeyDepartment of Analytical Chemistry, Faculty of Pharmacy, Ege University, Izmir 35100, TurkeyDepartment of Biomedical Engineering, Izmir University of Economics, Izmir 35330, TurkeyDepartment of Analytical Chemistry, Faculty of Pharmacy, Ege University, Izmir 35100, TurkeyA handheld USB-powered instrument developed for the electrochemical detection of nucleic acids and biomolecular interactions is presented. The proposed instrument is capable of scanning ± 2.25 V while measuring currents up to ±10 mA, with a minimum current resolution of 6.87 pA. Therefore, it is suitable for nucleic acid sensors, which have high background currents. A low-cost microcontroller with an on-chip 16-bit analog-to-digital converter, 12-bit digital-to-analog converter, and a built-in USB controller were used to miniaturize the system. The offset voltages and gain errors of the analog peripherals were calibrated to obtain a superior performance. Thus, a similar performance to those of the market-leader potentiostats was achieved, but at a fraction of their cost and size. The performance of the application of this proposed architecture was tested successfully and was found to be similar to a leading commercial device through a clinical application in the aspects of the detection of nucleic acids, such as calf thymus ssDNA and dsDNA, and their interactions with a protein (BSA) by using single-use graphite electrodes in combination with the differential pulse voltammetry technique.https://www.mdpi.com/2072-666X/13/10/1610potentiostatdifferential pulse voltammetrypoint-of-care testing |
spellingShingle | Alper Demirhan Ece Eksin Yalin Kilic Arzum Erdem Low-Cost High-Resolution Potentiostat for Electrochemical Detection of Nucleic Acids and Biomolecular Interactions Micromachines potentiostat differential pulse voltammetry point-of-care testing |
title | Low-Cost High-Resolution Potentiostat for Electrochemical Detection of Nucleic Acids and Biomolecular Interactions |
title_full | Low-Cost High-Resolution Potentiostat for Electrochemical Detection of Nucleic Acids and Biomolecular Interactions |
title_fullStr | Low-Cost High-Resolution Potentiostat for Electrochemical Detection of Nucleic Acids and Biomolecular Interactions |
title_full_unstemmed | Low-Cost High-Resolution Potentiostat for Electrochemical Detection of Nucleic Acids and Biomolecular Interactions |
title_short | Low-Cost High-Resolution Potentiostat for Electrochemical Detection of Nucleic Acids and Biomolecular Interactions |
title_sort | low cost high resolution potentiostat for electrochemical detection of nucleic acids and biomolecular interactions |
topic | potentiostat differential pulse voltammetry point-of-care testing |
url | https://www.mdpi.com/2072-666X/13/10/1610 |
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