dCAPS method: advantages, troubles and solution
In our work, we focus on the evolutionary studies of sex chromosomes. As model organisms we use several species of the plant genus Silene. An important part of our research is represented by genetic mapping based on the assays of DNA length or sequence polymorphisms. Apart from the other methods we...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2007-09-01
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| Series: | Plant, Soil and Environment |
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| Online Access: | https://pse.agriculturejournals.cz/artkey/pse-200709-0007_dcaps-method-advantages-troubles-and-solution.php |
| _version_ | 1797898377299492864 |
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| author | M. Hrubá |
| author_facet | M. Hrubá |
| author_sort | M. Hrubá |
| collection | DOAJ |
| description | In our work, we focus on the evolutionary studies of sex chromosomes. As model organisms we use several species of the plant genus Silene. An important part of our research is represented by genetic mapping based on the assays of DNA length or sequence polymorphisms. Apart from the other methods we also use the dCAPS method, which is very useful for detection of the sequence polymorphisms (SNPs). This method is unique as it is able to detect SNPs that are not situated in any restriction site; a fundamental principle of this method is usage of primer designed with one or two mismatches that bring into the target sequence the mutation in vicinity of SNP. Using this method, we found out some improvements that can make analyses more cost-effective. |
| first_indexed | 2024-04-10T08:12:28Z |
| format | Article |
| id | doaj.art-d8d310037cd1468aa35e82aa946c9802 |
| institution | Directory Open Access Journal |
| issn | 1214-1178 1805-9368 |
| language | English |
| last_indexed | 2024-04-10T08:12:28Z |
| publishDate | 2007-09-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Plant, Soil and Environment |
| spelling | doaj.art-d8d310037cd1468aa35e82aa946c98022023-02-23T03:45:19ZengCzech Academy of Agricultural SciencesPlant, Soil and Environment1214-11781805-93682007-09-0153941742010.17221/2293-PSEpse-200709-0007dCAPS method: advantages, troubles and solutionM. Hrubá0Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech RepublicIn our work, we focus on the evolutionary studies of sex chromosomes. As model organisms we use several species of the plant genus Silene. An important part of our research is represented by genetic mapping based on the assays of DNA length or sequence polymorphisms. Apart from the other methods we also use the dCAPS method, which is very useful for detection of the sequence polymorphisms (SNPs). This method is unique as it is able to detect SNPs that are not situated in any restriction site; a fundamental principle of this method is usage of primer designed with one or two mismatches that bring into the target sequence the mutation in vicinity of SNP. Using this method, we found out some improvements that can make analyses more cost-effective.https://pse.agriculturejournals.cz/artkey/pse-200709-0007_dcaps-method-advantages-troubles-and-solution.phpdetection of polymorphismsnprestriction endonucleasesemi-nested pcrdna agargenetic mappingsilene |
| spellingShingle | M. Hrubá dCAPS method: advantages, troubles and solution Plant, Soil and Environment detection of polymorphism snp restriction endonuclease semi-nested pcr dna agar genetic mapping silene |
| title | dCAPS method: advantages, troubles and solution |
| title_full | dCAPS method: advantages, troubles and solution |
| title_fullStr | dCAPS method: advantages, troubles and solution |
| title_full_unstemmed | dCAPS method: advantages, troubles and solution |
| title_short | dCAPS method: advantages, troubles and solution |
| title_sort | dcaps method advantages troubles and solution |
| topic | detection of polymorphism snp restriction endonuclease semi-nested pcr dna agar genetic mapping silene |
| url | https://pse.agriculturejournals.cz/artkey/pse-200709-0007_dcaps-method-advantages-troubles-and-solution.php |
| work_keys_str_mv | AT mhruba dcapsmethodadvantagestroublesandsolution |