Application of <i>Pichia kudriavzevii</i> NBRC1279 and NBRC1664 to Simultaneous Saccharification and Fermentation for Bioethanol Production
Simultaneous saccharification and fermentation (SSF) is capable of performing enzymatic saccharification and fermentation for biofuel production in a single vessel. Thus, SSF has several advantages such as simplifying the manufacturing process, operating easily, and reducing energy input. Here, we d...
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MDPI AG
2021-05-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/7/2/83 |
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| author | Hironaga Akita Tetsuya Goshima Toshihiro Suzuki Yuya Itoiri Zen-ichiro Kimura Akinori Matsushika |
| author_facet | Hironaga Akita Tetsuya Goshima Toshihiro Suzuki Yuya Itoiri Zen-ichiro Kimura Akinori Matsushika |
| author_sort | Hironaga Akita |
| collection | DOAJ |
| description | Simultaneous saccharification and fermentation (SSF) is capable of performing enzymatic saccharification and fermentation for biofuel production in a single vessel. Thus, SSF has several advantages such as simplifying the manufacturing process, operating easily, and reducing energy input. Here, we describe the application of <i>Pichia kudriavzevii</i> NBRC1279 and NBRC1664 to SSF for bioethanol production. When each strain was incubated for 144 h at 35 °C with Japanese cedar particles, the highest ethanol concentrations were reached 21.9 ± 0.50 g/L and 23.8 ± 3.9 g/L, respectively. In addition, 21.6 ± 0.29 g/L and 21.3 ± 0.21 g/L of bioethanol were produced from Japanese eucalyptus particles when each strain was incubated for 144 h at 30 °C. Although previous methods require pretreatment of the source material, our method does not require pretreatment, which is an advantage for industrial use. To elucidate the different characteristics of the strains, we performed genome sequencing and genome comparison. Based on the results of the eggNOG categories and the resulting Venn diagram, the functional abilities of both strains were similar. However, strain NBRC1279 showed five retrotransposon protein genes in the draft genome sequence, which indicated that the stress tolerance of both strains is slightly different. |
| first_indexed | 2024-03-10T10:59:51Z |
| format | Article |
| id | doaj.art-d8d317e0a54a4cb883f8dcb2ba38761a |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-10T10:59:51Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-d8d317e0a54a4cb883f8dcb2ba38761a2023-11-21T21:36:26ZengMDPI AGFermentation2311-56372021-05-01728310.3390/fermentation7020083Application of <i>Pichia kudriavzevii</i> NBRC1279 and NBRC1664 to Simultaneous Saccharification and Fermentation for Bioethanol ProductionHironaga Akita0Tetsuya Goshima1Toshihiro Suzuki2Yuya Itoiri3Zen-ichiro Kimura4Akinori Matsushika5Research Institute for Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-0046, JapanNational Research Institute of Brewing, 3-7-1 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-0046, JapanResearch Institute for Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-0046, JapanDepartment of Civil and Environmental Engineering, National Institute of Technology, Kure College, 2-2-11 Aga-minami, Kure, Hiroshima 737-8506, JapanDepartment of Civil and Environmental Engineering, National Institute of Technology, Kure College, 2-2-11 Aga-minami, Kure, Hiroshima 737-8506, JapanResearch Institute for Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-0046, JapanSimultaneous saccharification and fermentation (SSF) is capable of performing enzymatic saccharification and fermentation for biofuel production in a single vessel. Thus, SSF has several advantages such as simplifying the manufacturing process, operating easily, and reducing energy input. Here, we describe the application of <i>Pichia kudriavzevii</i> NBRC1279 and NBRC1664 to SSF for bioethanol production. When each strain was incubated for 144 h at 35 °C with Japanese cedar particles, the highest ethanol concentrations were reached 21.9 ± 0.50 g/L and 23.8 ± 3.9 g/L, respectively. In addition, 21.6 ± 0.29 g/L and 21.3 ± 0.21 g/L of bioethanol were produced from Japanese eucalyptus particles when each strain was incubated for 144 h at 30 °C. Although previous methods require pretreatment of the source material, our method does not require pretreatment, which is an advantage for industrial use. To elucidate the different characteristics of the strains, we performed genome sequencing and genome comparison. Based on the results of the eggNOG categories and the resulting Venn diagram, the functional abilities of both strains were similar. However, strain NBRC1279 showed five retrotransposon protein genes in the draft genome sequence, which indicated that the stress tolerance of both strains is slightly different.https://www.mdpi.com/2311-5637/7/2/83<i>Pichia kudriavzevii</i>NBRC strainSSFbioethanolOptimash BGAcremonium cellulase |
| spellingShingle | Hironaga Akita Tetsuya Goshima Toshihiro Suzuki Yuya Itoiri Zen-ichiro Kimura Akinori Matsushika Application of <i>Pichia kudriavzevii</i> NBRC1279 and NBRC1664 to Simultaneous Saccharification and Fermentation for Bioethanol Production Fermentation <i>Pichia kudriavzevii</i> NBRC strain SSF bioethanol Optimash BG Acremonium cellulase |
| title | Application of <i>Pichia kudriavzevii</i> NBRC1279 and NBRC1664 to Simultaneous Saccharification and Fermentation for Bioethanol Production |
| title_full | Application of <i>Pichia kudriavzevii</i> NBRC1279 and NBRC1664 to Simultaneous Saccharification and Fermentation for Bioethanol Production |
| title_fullStr | Application of <i>Pichia kudriavzevii</i> NBRC1279 and NBRC1664 to Simultaneous Saccharification and Fermentation for Bioethanol Production |
| title_full_unstemmed | Application of <i>Pichia kudriavzevii</i> NBRC1279 and NBRC1664 to Simultaneous Saccharification and Fermentation for Bioethanol Production |
| title_short | Application of <i>Pichia kudriavzevii</i> NBRC1279 and NBRC1664 to Simultaneous Saccharification and Fermentation for Bioethanol Production |
| title_sort | application of i pichia kudriavzevii i nbrc1279 and nbrc1664 to simultaneous saccharification and fermentation for bioethanol production |
| topic | <i>Pichia kudriavzevii</i> NBRC strain SSF bioethanol Optimash BG Acremonium cellulase |
| url | https://www.mdpi.com/2311-5637/7/2/83 |
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