Altered microbiome of serum exosomes in patients with acute and chronic cholecystitis
Abstract Background This study aimed to investigate the differences in the microbiota composition of serum exosomes from patients with acute and chronic cholecystitis. Method Exosomes were isolated from the serum of cholecystitis patients through centrifugation and identified and characterized using...
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BMC
2024-04-01
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| Series: | BMC Microbiology |
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| Online Access: | https://doi.org/10.1186/s12866-024-03269-6 |
| _version_ | 1827277318919290880 |
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| author | Qing Zhu Min-Xian Li Ming-Chin Yu Qi-Wen Ma Ming-Jie Huang Chun-Wei Lu Chun-Bing Chen Wen-Hung Chung Chih-Jung Chang |
| author_facet | Qing Zhu Min-Xian Li Ming-Chin Yu Qi-Wen Ma Ming-Jie Huang Chun-Wei Lu Chun-Bing Chen Wen-Hung Chung Chih-Jung Chang |
| author_sort | Qing Zhu |
| collection | DOAJ |
| description | Abstract Background This study aimed to investigate the differences in the microbiota composition of serum exosomes from patients with acute and chronic cholecystitis. Method Exosomes were isolated from the serum of cholecystitis patients through centrifugation and identified and characterized using transmission electron microscopy and nano-flow cytometry. Microbiota analysis was performed using 16S rRNA sequencing. Results Compared to patients with chronic cholecystitis, those with acute cholecystitis exhibited lower richness and diversity. Beta diversity analysis revealed significant differences in the microbiota composition between patients with acute and chronic cholecystitis. The relative abundance of Proteobacteria was significantly higher in exosomes from patients with acute cholecystitis, whereas Actinobacteria, Bacteroidetes, and Firmicutes were significantly more abundant in exosomes from patients with chronic cholecystitis. Furthermore, functional predictions of microbial communities using Tax4Fun analysis revealed significant differences in metabolic pathways such as amino acid metabolism, carbohydrate metabolism, and membrane transport between the two patient groups. Conclusions This study confirmed the differences in the microbiota composition within serum exosomes of patients with acute and chronic cholecystitis. Serum exosomes could serve as diagnostic indicators for distinguishing acute and chronic cholecystitis. |
| first_indexed | 2024-04-24T07:18:55Z |
| format | Article |
| id | doaj.art-d8d47e4ce40040b08022f21d3d0b91fe |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-04-24T07:18:55Z |
| publishDate | 2024-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-d8d47e4ce40040b08022f21d3d0b91fe2024-04-21T11:10:42ZengBMCBMC Microbiology1471-21802024-04-0124111210.1186/s12866-024-03269-6Altered microbiome of serum exosomes in patients with acute and chronic cholecystitisQing Zhu0Min-Xian Li1Ming-Chin Yu2Qi-Wen Ma3Ming-Jie Huang4Chun-Wei Lu5Chun-Bing Chen6Wen-Hung Chung7Chih-Jung Chang8Department of Surgery, Xiamen Chang Gung Hospital Hua Qiao UniversityDepartment of Surgery, Xiamen Chang Gung Hospital Hua Qiao UniversityDepartment of Surgery, Xiamen Chang Gung Hospital Hua Qiao UniversitySchool of Medicine and Medical Research Center, Xiamen Chang Gung Hospital Hua Qiao UniversitySchool of Medicine and Medical Research Center, Xiamen Chang Gung Hospital Hua Qiao UniversityDrug Hypersensitivity Clinical and Research Center, Department of Dermatology, Chang Gung Memorial HospitalDrug Hypersensitivity Clinical and Research Center, Department of Dermatology, Chang Gung Memorial HospitalSchool of Medicine and Medical Research Center, Xiamen Chang Gung Hospital Hua Qiao UniversitySchool of Medicine and Medical Research Center, Xiamen Chang Gung Hospital Hua Qiao UniversityAbstract Background This study aimed to investigate the differences in the microbiota composition of serum exosomes from patients with acute and chronic cholecystitis. Method Exosomes were isolated from the serum of cholecystitis patients through centrifugation and identified and characterized using transmission electron microscopy and nano-flow cytometry. Microbiota analysis was performed using 16S rRNA sequencing. Results Compared to patients with chronic cholecystitis, those with acute cholecystitis exhibited lower richness and diversity. Beta diversity analysis revealed significant differences in the microbiota composition between patients with acute and chronic cholecystitis. The relative abundance of Proteobacteria was significantly higher in exosomes from patients with acute cholecystitis, whereas Actinobacteria, Bacteroidetes, and Firmicutes were significantly more abundant in exosomes from patients with chronic cholecystitis. Furthermore, functional predictions of microbial communities using Tax4Fun analysis revealed significant differences in metabolic pathways such as amino acid metabolism, carbohydrate metabolism, and membrane transport between the two patient groups. Conclusions This study confirmed the differences in the microbiota composition within serum exosomes of patients with acute and chronic cholecystitis. Serum exosomes could serve as diagnostic indicators for distinguishing acute and chronic cholecystitis.https://doi.org/10.1186/s12866-024-03269-6CholecystitisExosomesMicrobiota composition16S rRNA sequencing |
| spellingShingle | Qing Zhu Min-Xian Li Ming-Chin Yu Qi-Wen Ma Ming-Jie Huang Chun-Wei Lu Chun-Bing Chen Wen-Hung Chung Chih-Jung Chang Altered microbiome of serum exosomes in patients with acute and chronic cholecystitis BMC Microbiology Cholecystitis Exosomes Microbiota composition 16S rRNA sequencing |
| title | Altered microbiome of serum exosomes in patients with acute and chronic cholecystitis |
| title_full | Altered microbiome of serum exosomes in patients with acute and chronic cholecystitis |
| title_fullStr | Altered microbiome of serum exosomes in patients with acute and chronic cholecystitis |
| title_full_unstemmed | Altered microbiome of serum exosomes in patients with acute and chronic cholecystitis |
| title_short | Altered microbiome of serum exosomes in patients with acute and chronic cholecystitis |
| title_sort | altered microbiome of serum exosomes in patients with acute and chronic cholecystitis |
| topic | Cholecystitis Exosomes Microbiota composition 16S rRNA sequencing |
| url | https://doi.org/10.1186/s12866-024-03269-6 |
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