Salivary Gland Dysfunction, Protein Glycooxidation and Nitrosative Stress in Children with Chronic Kidney Disease
This study is the first to evaluate protein glycooxidation products, lipid oxidative damage and nitrosative stress in non-stimulated (NWS) and stimulated whole saliva (SWS) of children with chronic kidney disease (CKD) divided into two subgroups: normal salivary secretion (<i>n</i> = 18)...
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MDPI AG
2020-04-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/9/5/1285 |
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| author | Mateusz Maciejczyk Julita Szulimowska Katarzyna Taranta-Janusz Anna Wasilewska Anna Zalewska |
| author_facet | Mateusz Maciejczyk Julita Szulimowska Katarzyna Taranta-Janusz Anna Wasilewska Anna Zalewska |
| author_sort | Mateusz Maciejczyk |
| collection | DOAJ |
| description | This study is the first to evaluate protein glycooxidation products, lipid oxidative damage and nitrosative stress in non-stimulated (NWS) and stimulated whole saliva (SWS) of children with chronic kidney disease (CKD) divided into two subgroups: normal salivary secretion (<i>n</i> = 18) and hyposalivation (NWS flow < 0.2 mL min<sup>−1</sup>; <i>n</i> = 12). Hyposalivation was observed in all patients with severe renal failure (4–5 stage CKD), while saliva secretion > 0.2 mL/min in children with mild-moderate CKD (1–3 stage) and controls. Salivary amylase activity and total protein content were significantly lower in CKD children with hyposalivation compared to CKD patients with normal saliva secretion and control group. The fluorescence of protein glycooxidation products (kynurenine, N-formylkynurenine, advanced glycation end products), the content of oxidative damage to lipids (4-hydroxynonneal, 8-isoprostanes) and nitrosative stress (peroxynitrite, nitrotyrosine) were significantly higher in NWS, SWS, and plasma of CKD children with hyposalivation compared to patients with normal salivary secretion and healthy controls. In CKD group, salivary oxidation products correlated negatively with salivary flow rate, α-amylase activity and total protein content; however, salivary oxidation products do not reflect their plasma level. In conclusion, children with CKD suffer from salivary gland dysfunction. Oxidation of salivary proteins and lipids increases with CKD progression and deterioration of salivary gland function. |
| first_indexed | 2024-03-10T20:09:54Z |
| format | Article |
| id | doaj.art-d8d545f156254be2bff016250112dc33 |
| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-10T20:09:54Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-d8d545f156254be2bff016250112dc332023-11-19T23:01:23ZengMDPI AGJournal of Clinical Medicine2077-03832020-04-0195128510.3390/jcm9051285Salivary Gland Dysfunction, Protein Glycooxidation and Nitrosative Stress in Children with Chronic Kidney DiseaseMateusz Maciejczyk0Julita Szulimowska1Katarzyna Taranta-Janusz2Anna Wasilewska3Anna Zalewska4Department of Hygiene, Epidemiology and Ergonomics, Medical University of Bialystok, 2c Mickiewicza Street, 15-233 Bialystok, PolandDepartment of Pedodontics, Medical University of Bialystok, 24a M. Sklodowskiej-Curie Street, 15-274 Bialystok, PolandDepartment of Pediatrics and Nephrology, Medical University of Bialystok, 24a M. Sklodowskiej-Curie Street, 15-274 Bialystok, PolandDepartment of Pediatrics and Nephrology, Medical University of Bialystok, 24a M. Sklodowskiej-Curie Street, 15-274 Bialystok, PolandExperimental Dentistry Laboratory, Medical University of Bialystok, 24a M. Sklodowskiej-Curie Street, 15-274 Bialystok, PolandThis study is the first to evaluate protein glycooxidation products, lipid oxidative damage and nitrosative stress in non-stimulated (NWS) and stimulated whole saliva (SWS) of children with chronic kidney disease (CKD) divided into two subgroups: normal salivary secretion (<i>n</i> = 18) and hyposalivation (NWS flow < 0.2 mL min<sup>−1</sup>; <i>n</i> = 12). Hyposalivation was observed in all patients with severe renal failure (4–5 stage CKD), while saliva secretion > 0.2 mL/min in children with mild-moderate CKD (1–3 stage) and controls. Salivary amylase activity and total protein content were significantly lower in CKD children with hyposalivation compared to CKD patients with normal saliva secretion and control group. The fluorescence of protein glycooxidation products (kynurenine, N-formylkynurenine, advanced glycation end products), the content of oxidative damage to lipids (4-hydroxynonneal, 8-isoprostanes) and nitrosative stress (peroxynitrite, nitrotyrosine) were significantly higher in NWS, SWS, and plasma of CKD children with hyposalivation compared to patients with normal salivary secretion and healthy controls. In CKD group, salivary oxidation products correlated negatively with salivary flow rate, α-amylase activity and total protein content; however, salivary oxidation products do not reflect their plasma level. In conclusion, children with CKD suffer from salivary gland dysfunction. Oxidation of salivary proteins and lipids increases with CKD progression and deterioration of salivary gland function.https://www.mdpi.com/2077-0383/9/5/1285chronic kidney diseasesalivary gland dysfunctionsalivary biomarkersoxidative stressnitrosative stress |
| spellingShingle | Mateusz Maciejczyk Julita Szulimowska Katarzyna Taranta-Janusz Anna Wasilewska Anna Zalewska Salivary Gland Dysfunction, Protein Glycooxidation and Nitrosative Stress in Children with Chronic Kidney Disease Journal of Clinical Medicine chronic kidney disease salivary gland dysfunction salivary biomarkers oxidative stress nitrosative stress |
| title | Salivary Gland Dysfunction, Protein Glycooxidation and Nitrosative Stress in Children with Chronic Kidney Disease |
| title_full | Salivary Gland Dysfunction, Protein Glycooxidation and Nitrosative Stress in Children with Chronic Kidney Disease |
| title_fullStr | Salivary Gland Dysfunction, Protein Glycooxidation and Nitrosative Stress in Children with Chronic Kidney Disease |
| title_full_unstemmed | Salivary Gland Dysfunction, Protein Glycooxidation and Nitrosative Stress in Children with Chronic Kidney Disease |
| title_short | Salivary Gland Dysfunction, Protein Glycooxidation and Nitrosative Stress in Children with Chronic Kidney Disease |
| title_sort | salivary gland dysfunction protein glycooxidation and nitrosative stress in children with chronic kidney disease |
| topic | chronic kidney disease salivary gland dysfunction salivary biomarkers oxidative stress nitrosative stress |
| url | https://www.mdpi.com/2077-0383/9/5/1285 |
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