Endogenous leptin promotes autophagy in EBSS-induced PFCs
Leptin is an important adipokine and plays a vital role in animals. However, the role of leptin in the autophagic response of pig fibroblast cells (PFCs) has not been fully elucidated. In this study, we investigated the relationship between leptin and autophagy as well as underlying molecular basis....
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-09-01
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| Series: | Animal Cells and Systems |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/19768354.2019.1651766 |
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| author | Deling Jiao Zhen Yang Lulu Wang Binyue Hu Jing Wang Anyong Xu Wenmin Cheng Baoyu Jia Yubo Qing Hong-Ye Zhao Hong-Jiang Wei |
| author_facet | Deling Jiao Zhen Yang Lulu Wang Binyue Hu Jing Wang Anyong Xu Wenmin Cheng Baoyu Jia Yubo Qing Hong-Ye Zhao Hong-Jiang Wei |
| author_sort | Deling Jiao |
| collection | DOAJ |
| description | Leptin is an important adipokine and plays a vital role in animals. However, the role of leptin in the autophagic response of pig fibroblast cells (PFCs) has not been fully elucidated. In this study, we investigated the relationship between leptin and autophagy as well as underlying molecular basis. We found that PFCs treated with EBSS could secrete leptin, and the leptin concentration in the supernatant of leptin transgenic PFCs was higher than that of WT PFCs. We found an increase in LC3-II protein level and a decrease in p62 protein level in treated leptin transgenic PFCs compared with treated WT PFCs. Meanwhile, we observed an increase of autophagosomes by transmission electron microscopy and an enhancement of the accumulation of LC3 puncta in the cytoplasm of treated leptin transgenic PFCs, and these effects were further augmented by Baf A1 treatment. Furthermore, we detected the expression levels of 7 autophagy signaling pathway genes and 17 autophagy-related (ATG) genes by q-PCR. We found that between the two types of EBSS-treated cells 3 genes expression pattern were significantly different among the 7 autophagy signaling pathway genes and 8 genes expression pattern were significantly differernt among the ATG genes. These results indicated that leptin may promote autophagy and involving the downregulation of FOXO1 and LMNA genes via an unknown pathway which causes the upregulation of the 4 genes and the downregulation of 4 genes. |
| first_indexed | 2024-04-12T01:52:06Z |
| format | Article |
| id | doaj.art-d8d65fad5ad049f0affb21b9294ce07f |
| institution | Directory Open Access Journal |
| issn | 1976-8354 2151-2485 |
| language | English |
| last_indexed | 2024-04-12T01:52:06Z |
| publishDate | 2019-09-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Animal Cells and Systems |
| spelling | doaj.art-d8d65fad5ad049f0affb21b9294ce07f2022-12-22T03:52:55ZengTaylor & Francis GroupAnimal Cells and Systems1976-83542151-24852019-09-0123531832510.1080/19768354.2019.16517661651766Endogenous leptin promotes autophagy in EBSS-induced PFCsDeling Jiao0Zhen Yang1Lulu Wang2Binyue Hu3Jing Wang4Anyong Xu5Wenmin Cheng6Baoyu Jia7Yubo Qing8Hong-Ye Zhao9Hong-Jiang Wei10Key Laboratory of Animal Gene Editing and Animal Cloning in Yunnan ProvinceKey Laboratory of Animal Gene Editing and Animal Cloning in Yunnan ProvinceKey Laboratory of Animal Gene Editing and Animal Cloning in Yunnan ProvinceYunnan Agricultural UniversityKey Laboratory of Animal Gene Editing and Animal Cloning in Yunnan ProvinceYunnan Agricultural UniversityKey Laboratory of Animal Gene Editing and Animal Cloning in Yunnan ProvinceKey Laboratory of Animal Gene Editing and Animal Cloning in Yunnan ProvinceKey Laboratory of Animal Gene Editing and Animal Cloning in Yunnan ProvinceKey Laboratory of Animal Gene Editing and Animal Cloning in Yunnan ProvinceKey Laboratory of Animal Gene Editing and Animal Cloning in Yunnan ProvinceLeptin is an important adipokine and plays a vital role in animals. However, the role of leptin in the autophagic response of pig fibroblast cells (PFCs) has not been fully elucidated. In this study, we investigated the relationship between leptin and autophagy as well as underlying molecular basis. We found that PFCs treated with EBSS could secrete leptin, and the leptin concentration in the supernatant of leptin transgenic PFCs was higher than that of WT PFCs. We found an increase in LC3-II protein level and a decrease in p62 protein level in treated leptin transgenic PFCs compared with treated WT PFCs. Meanwhile, we observed an increase of autophagosomes by transmission electron microscopy and an enhancement of the accumulation of LC3 puncta in the cytoplasm of treated leptin transgenic PFCs, and these effects were further augmented by Baf A1 treatment. Furthermore, we detected the expression levels of 7 autophagy signaling pathway genes and 17 autophagy-related (ATG) genes by q-PCR. We found that between the two types of EBSS-treated cells 3 genes expression pattern were significantly different among the 7 autophagy signaling pathway genes and 8 genes expression pattern were significantly differernt among the ATG genes. These results indicated that leptin may promote autophagy and involving the downregulation of FOXO1 and LMNA genes via an unknown pathway which causes the upregulation of the 4 genes and the downregulation of 4 genes.http://dx.doi.org/10.1080/19768354.2019.1651766leptinautophagypfcsautophagy-related genesautophagy signaling pathway-related genes |
| spellingShingle | Deling Jiao Zhen Yang Lulu Wang Binyue Hu Jing Wang Anyong Xu Wenmin Cheng Baoyu Jia Yubo Qing Hong-Ye Zhao Hong-Jiang Wei Endogenous leptin promotes autophagy in EBSS-induced PFCs Animal Cells and Systems leptin autophagy pfcs autophagy-related genes autophagy signaling pathway-related genes |
| title | Endogenous leptin promotes autophagy in EBSS-induced PFCs |
| title_full | Endogenous leptin promotes autophagy in EBSS-induced PFCs |
| title_fullStr | Endogenous leptin promotes autophagy in EBSS-induced PFCs |
| title_full_unstemmed | Endogenous leptin promotes autophagy in EBSS-induced PFCs |
| title_short | Endogenous leptin promotes autophagy in EBSS-induced PFCs |
| title_sort | endogenous leptin promotes autophagy in ebss induced pfcs |
| topic | leptin autophagy pfcs autophagy-related genes autophagy signaling pathway-related genes |
| url | http://dx.doi.org/10.1080/19768354.2019.1651766 |
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