Astilbe Chinensis ethanol extract suppresses inflammation in macrophages via NF-κB pathway
Abstract Background Macrophages play a crucial role in inflammation. Astilbe chinensis is one of perennial herbs belonging to the genus Astilbe. Plants in the genus have been used for pain, headaches, arthralgia, and chronic bronchitis. However, the effect of A.chinensis on inflammation remains uncl...
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| Format: | Article |
| Language: | English |
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BMC
2020-10-01
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| Series: | BMC Complementary Medicine and Therapies |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12906-020-03073-5 |
| _version_ | 1818199090982289408 |
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| author | Tae-Young Gil Bo-Ram Jin Chul-Hee Hong Jong Hyuk Park Hyo-Jin An |
| author_facet | Tae-Young Gil Bo-Ram Jin Chul-Hee Hong Jong Hyuk Park Hyo-Jin An |
| author_sort | Tae-Young Gil |
| collection | DOAJ |
| description | Abstract Background Macrophages play a crucial role in inflammation. Astilbe chinensis is one of perennial herbs belonging to the genus Astilbe. Plants in the genus have been used for pain, headaches, arthralgia, and chronic bronchitis. However, the effect of A.chinensis on inflammation remains unclear. To study the anti-inflammatory action of A.chinensis ethanol extract (ACE), we investigated the effect of ACE on the production of pro-inflammatory mediators and cytokines in macrophages. Methods We evaluated the effectiveness of ACE in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages and thioglycollate (TG)-elicited peritoneal macrophages from male C57BL/6 mice. We measured the levels of pro-inflammatory mediators and cytokines, and examined the anti-inflammatory actions of ACE on nuclear factor κB (NF-κB) pathway in the macrophages. Western blot analysis and immunofluorescence microscopy were used to determine protein level and translocation, respectively. Results ACE suppressed the output of nitric oxide (NO), prostaglandin E2 (PGE2), and pro-inflammatory cytokines in stimulated macrophages via inhibiting the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) proteins. ACE suppressed mRNA expression of pro-inflammatory cytokines such as interleukin (IL)-6 and tumor necrosis factor-alpha (TNF-α). We examined the efficacies of ACE on NF-κB activation by measuring the expressions including IκB kinase (IKK), inhibitor of κB (IκB), and nuclear p65 proteins. In addition, the inhibition of NF-κB p65’s translocation was determined with immunofluorescence assay. Conclusion Our findings manifested that ACE inhibited LPS or TG-induced inflammation by blocking the NF-κB signaling pathway in macrophages. It indicated that ACE is a potential therapeutic mean for inflammation and related diseases. |
| first_indexed | 2024-12-12T02:16:15Z |
| format | Article |
| id | doaj.art-d8d6e35e2d9e4edfb775251aaa929785 |
| institution | Directory Open Access Journal |
| issn | 2662-7671 |
| language | English |
| last_indexed | 2024-12-12T02:16:15Z |
| publishDate | 2020-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary Medicine and Therapies |
| spelling | doaj.art-d8d6e35e2d9e4edfb775251aaa9297852022-12-22T00:41:48ZengBMCBMC Complementary Medicine and Therapies2662-76712020-10-0120111110.1186/s12906-020-03073-5Astilbe Chinensis ethanol extract suppresses inflammation in macrophages via NF-κB pathwayTae-Young Gil0Bo-Ram Jin1Chul-Hee Hong2Jong Hyuk Park3Hyo-Jin An4Department of Pharmacology, College of Korean Medicine, Sangji UniversityDepartment of Pharmacology, College of Korean Medicine, Sangji UniversityDepartment of Korean Medicine Ophthalmology & Otolaryngology & Dermatology, College of Korean Medicine, Sangji UniversityHallym UniversityDepartment of Pharmacology, College of Korean Medicine, Sangji UniversityAbstract Background Macrophages play a crucial role in inflammation. Astilbe chinensis is one of perennial herbs belonging to the genus Astilbe. Plants in the genus have been used for pain, headaches, arthralgia, and chronic bronchitis. However, the effect of A.chinensis on inflammation remains unclear. To study the anti-inflammatory action of A.chinensis ethanol extract (ACE), we investigated the effect of ACE on the production of pro-inflammatory mediators and cytokines in macrophages. Methods We evaluated the effectiveness of ACE in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages and thioglycollate (TG)-elicited peritoneal macrophages from male C57BL/6 mice. We measured the levels of pro-inflammatory mediators and cytokines, and examined the anti-inflammatory actions of ACE on nuclear factor κB (NF-κB) pathway in the macrophages. Western blot analysis and immunofluorescence microscopy were used to determine protein level and translocation, respectively. Results ACE suppressed the output of nitric oxide (NO), prostaglandin E2 (PGE2), and pro-inflammatory cytokines in stimulated macrophages via inhibiting the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) proteins. ACE suppressed mRNA expression of pro-inflammatory cytokines such as interleukin (IL)-6 and tumor necrosis factor-alpha (TNF-α). We examined the efficacies of ACE on NF-κB activation by measuring the expressions including IκB kinase (IKK), inhibitor of κB (IκB), and nuclear p65 proteins. In addition, the inhibition of NF-κB p65’s translocation was determined with immunofluorescence assay. Conclusion Our findings manifested that ACE inhibited LPS or TG-induced inflammation by blocking the NF-κB signaling pathway in macrophages. It indicated that ACE is a potential therapeutic mean for inflammation and related diseases.http://link.springer.com/article/10.1186/s12906-020-03073-5Astilbe chinensisMacrophagesNF-κB pathwayInflammationIn vitroEx vivo |
| spellingShingle | Tae-Young Gil Bo-Ram Jin Chul-Hee Hong Jong Hyuk Park Hyo-Jin An Astilbe Chinensis ethanol extract suppresses inflammation in macrophages via NF-κB pathway BMC Complementary Medicine and Therapies Astilbe chinensis Macrophages NF-κB pathway Inflammation In vitro Ex vivo |
| title | Astilbe Chinensis ethanol extract suppresses inflammation in macrophages via NF-κB pathway |
| title_full | Astilbe Chinensis ethanol extract suppresses inflammation in macrophages via NF-κB pathway |
| title_fullStr | Astilbe Chinensis ethanol extract suppresses inflammation in macrophages via NF-κB pathway |
| title_full_unstemmed | Astilbe Chinensis ethanol extract suppresses inflammation in macrophages via NF-κB pathway |
| title_short | Astilbe Chinensis ethanol extract suppresses inflammation in macrophages via NF-κB pathway |
| title_sort | astilbe chinensis ethanol extract suppresses inflammation in macrophages via nf κb pathway |
| topic | Astilbe chinensis Macrophages NF-κB pathway Inflammation In vitro Ex vivo |
| url | http://link.springer.com/article/10.1186/s12906-020-03073-5 |
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