Genistein induces apoptosis of colon cancer cells by reversal of epithelial-to-mesenchymal via a Notch1/NF-κB/slug/E-cadherin pathway
Abstract Background Genistein has been known to inhibit proliferation and induce apoptosis in several kinds of cancer cells. While knowledge of genistein in regulating epithelial mesenchymal transition (EMT) of colon cancer cells is unknown. Methods To investigate the effects and mechanisms of genis...
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| Format: | Article |
| Language: | English |
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BMC
2017-12-01
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| Series: | BMC Cancer |
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| Online Access: | http://link.springer.com/article/10.1186/s12885-017-3829-9 |
| _version_ | 1818504313758023680 |
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| author | Panpan Zhou Chunling Wang Zebin Hu Wenruo Chen Wentao Qi Aike Li |
| author_facet | Panpan Zhou Chunling Wang Zebin Hu Wenruo Chen Wentao Qi Aike Li |
| author_sort | Panpan Zhou |
| collection | DOAJ |
| description | Abstract Background Genistein has been known to inhibit proliferation and induce apoptosis in several kinds of cancer cells. While knowledge of genistein in regulating epithelial mesenchymal transition (EMT) of colon cancer cells is unknown. Methods To investigate the effects and mechanisms of genistein on EMT of colon cancer cells, HT-29 cells were used and treated by genistein and TNF-α in this paper. EMT was determined by cell invasion assays using a transwell chamber and the expression changes of EMT-related markers were confirmed by RT–PCR, Western blotting, and immunofluorescence staining. Results Genistein inhibited cell migration at 200 μmol/L. Genistein reversed the EMT of colon cancer cells by upregulation of E-cadherin and downregulation of N-cadherin, accompanied by the suppression of EMT related makers, such as Snail2/slug, ZEB1, ZEB2, FOXC1, FOXC2 and TWIST1. Moreover, genistein can inhibit the expression of notch-1, p-NF-κB and NF-κB, while promote the expression of Bax/Bcl-2 and caspase-3 in HT-29 cells. Conclusion The present study demonstrated that genistein suppressed the migration of colon cancer cells by reversal the EMT via suppressing the Notch1/NF-κB/slug/E-cadherin pathway. Genistein may be developed as a potential antimetastasis agent to colon cancer. |
| first_indexed | 2024-12-10T21:35:31Z |
| format | Article |
| id | doaj.art-d8d8530e7bea4e1ea06f6189acb2a44e |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-12-10T21:35:31Z |
| publishDate | 2017-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-d8d8530e7bea4e1ea06f6189acb2a44e2022-12-22T01:32:40ZengBMCBMC Cancer1471-24072017-12-0117111010.1186/s12885-017-3829-9Genistein induces apoptosis of colon cancer cells by reversal of epithelial-to-mesenchymal via a Notch1/NF-κB/slug/E-cadherin pathwayPanpan Zhou0Chunling Wang1Zebin Hu2Wenruo Chen3Wentao Qi4Aike Li5Cereals & Oils Nutrition Research Group, Academy of State Administration of Grain (ASAG)Key Laboratory of Food Safety and Sanitation, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and TechnologyInstitue for In Vitro Diagnostic Reagents Control, the National Institutes for food and drug Control (NIFDC)Cereals & Oils Nutrition Research Group, Academy of State Administration of Grain (ASAG)Cereals & Oils Nutrition Research Group, Academy of State Administration of Grain (ASAG)Cereals & Oils Nutrition Research Group, Academy of State Administration of Grain (ASAG)Abstract Background Genistein has been known to inhibit proliferation and induce apoptosis in several kinds of cancer cells. While knowledge of genistein in regulating epithelial mesenchymal transition (EMT) of colon cancer cells is unknown. Methods To investigate the effects and mechanisms of genistein on EMT of colon cancer cells, HT-29 cells were used and treated by genistein and TNF-α in this paper. EMT was determined by cell invasion assays using a transwell chamber and the expression changes of EMT-related markers were confirmed by RT–PCR, Western blotting, and immunofluorescence staining. Results Genistein inhibited cell migration at 200 μmol/L. Genistein reversed the EMT of colon cancer cells by upregulation of E-cadherin and downregulation of N-cadherin, accompanied by the suppression of EMT related makers, such as Snail2/slug, ZEB1, ZEB2, FOXC1, FOXC2 and TWIST1. Moreover, genistein can inhibit the expression of notch-1, p-NF-κB and NF-κB, while promote the expression of Bax/Bcl-2 and caspase-3 in HT-29 cells. Conclusion The present study demonstrated that genistein suppressed the migration of colon cancer cells by reversal the EMT via suppressing the Notch1/NF-κB/slug/E-cadherin pathway. Genistein may be developed as a potential antimetastasis agent to colon cancer.http://link.springer.com/article/10.1186/s12885-017-3829-9GenisteinColon cancer cellApoptosisEpithelial mesenchymal transition |
| spellingShingle | Panpan Zhou Chunling Wang Zebin Hu Wenruo Chen Wentao Qi Aike Li Genistein induces apoptosis of colon cancer cells by reversal of epithelial-to-mesenchymal via a Notch1/NF-κB/slug/E-cadherin pathway BMC Cancer Genistein Colon cancer cell Apoptosis Epithelial mesenchymal transition |
| title | Genistein induces apoptosis of colon cancer cells by reversal of epithelial-to-mesenchymal via a Notch1/NF-κB/slug/E-cadherin pathway |
| title_full | Genistein induces apoptosis of colon cancer cells by reversal of epithelial-to-mesenchymal via a Notch1/NF-κB/slug/E-cadherin pathway |
| title_fullStr | Genistein induces apoptosis of colon cancer cells by reversal of epithelial-to-mesenchymal via a Notch1/NF-κB/slug/E-cadherin pathway |
| title_full_unstemmed | Genistein induces apoptosis of colon cancer cells by reversal of epithelial-to-mesenchymal via a Notch1/NF-κB/slug/E-cadherin pathway |
| title_short | Genistein induces apoptosis of colon cancer cells by reversal of epithelial-to-mesenchymal via a Notch1/NF-κB/slug/E-cadherin pathway |
| title_sort | genistein induces apoptosis of colon cancer cells by reversal of epithelial to mesenchymal via a notch1 nf κb slug e cadherin pathway |
| topic | Genistein Colon cancer cell Apoptosis Epithelial mesenchymal transition |
| url | http://link.springer.com/article/10.1186/s12885-017-3829-9 |
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