A Volatile and Dynamic Longitudinal Microbiome Is Associated With Less Reduction in Lung Function in Adolescents With Cystic Fibrosis
Progressive impairment in lung function caused by chronic polymicrobial airway infection remains the major cause of death in patients with cystic fibrosis (CF). Cross-sectional studies suggest an association between lung function decline and specific lung microbiome ecotypes. However, longitudinal s...
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| Format: | Article |
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Frontiers Media S.A.
2021-12-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2021.763121/full |
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| author | Marisa I. Metzger Marisa I. Metzger Simon Y. Graeber Simon Y. Graeber Simon Y. Graeber Mirjam Stahl Mirjam Stahl Mirjam Stahl Olaf Sommerburg Olaf Sommerburg Olaf Sommerburg Marcus A. Mall Marcus A. Mall Marcus A. Mall Alexander H. Dalpke Sébastien Boutin Sébastien Boutin |
| author_facet | Marisa I. Metzger Marisa I. Metzger Simon Y. Graeber Simon Y. Graeber Simon Y. Graeber Mirjam Stahl Mirjam Stahl Mirjam Stahl Olaf Sommerburg Olaf Sommerburg Olaf Sommerburg Marcus A. Mall Marcus A. Mall Marcus A. Mall Alexander H. Dalpke Sébastien Boutin Sébastien Boutin |
| author_sort | Marisa I. Metzger |
| collection | DOAJ |
| description | Progressive impairment in lung function caused by chronic polymicrobial airway infection remains the major cause of death in patients with cystic fibrosis (CF). Cross-sectional studies suggest an association between lung function decline and specific lung microbiome ecotypes. However, longitudinal studies on the stability of the airway microbiome are missing for adolescents with CF constituting the age group showing the highest rate of decline in lung function. In this study, we analyzed longitudinal lung function data and sputum samples collected over a period of 3 to 5 years from 12 adolescents with CF. The sputum microbiome was analyzed using 16S rRNA gene sequencing. Our results indicate that the individual course of the lung microbiome is associated with longitudinal lung function. In our cohort, patients with a dynamic, diverse microbiome showed a slower decline of lung function measured by FEV1% predicted, whereas a more stable and less diverse lung microbiome was related to worse outcomes. Specifically, a higher abundance of the phyla Bacteroidetes and Firmicutes was linked to a better clinical outcome, while Proteobacteria were correlated with a decline in FEV1% predicted. Our study indicates that the stability and diversity of the lung microbiome and the abundance of Bacteroidetes and Firmicutes are associated with the lung function decline and are one of the contributing factors to the disease severity. |
| first_indexed | 2024-12-14T07:33:43Z |
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| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-14T07:33:43Z |
| publishDate | 2021-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-d8dabac938734fba8d47ad4436fc0d2e2022-12-21T23:11:17ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882021-12-011110.3389/fcimb.2021.763121763121A Volatile and Dynamic Longitudinal Microbiome Is Associated With Less Reduction in Lung Function in Adolescents With Cystic FibrosisMarisa I. Metzger0Marisa I. Metzger1Simon Y. Graeber2Simon Y. Graeber3Simon Y. Graeber4Mirjam Stahl5Mirjam Stahl6Mirjam Stahl7Olaf Sommerburg8Olaf Sommerburg9Olaf Sommerburg10Marcus A. Mall11Marcus A. Mall12Marcus A. Mall13Alexander H. Dalpke14Sébastien Boutin15Sébastien Boutin16Department of Infectious Diseases, Medical Microbiology and Hygiene, University of Heidelberg, Heidelberg, GermanyTranslational Lung Research Center Heidelberg (TLRC), German Center for Lung Research (DZL), University of Heidelberg, Heidelberg, GermanyGerman Centre for Lung Research (DZL), Associated Partner Site, Berlin, GermanyDepartment of Pediatric Respiratory Medicine, Immunology and Critical Care Medicine and Cystic Fibrosis Center, Charité - Universitätsmedizin Berlin, Berlin, GermanyBerlin Institute of Health (BIH), Berlin, GermanyGerman Centre for Lung Research (DZL), Associated Partner Site, Berlin, GermanyDepartment of Pediatric Respiratory Medicine, Immunology and Critical Care Medicine and Cystic Fibrosis Center, Charité - Universitätsmedizin Berlin, Berlin, GermanyBerlin Institute of Health (BIH), Berlin, GermanyTranslational Lung Research Center Heidelberg (TLRC), German Center for Lung Research (DZL), University of Heidelberg, Heidelberg, GermanyDivision of Pediatric Pulmonology & Allergy and Cystic Fibrosis Center, Department of Pediatrics, University of Heidelberg, Heidelberg, GermanyDepartment of Translational Pulmonology, University of Heidelberg, Heidelberg, GermanyGerman Centre for Lung Research (DZL), Associated Partner Site, Berlin, GermanyDepartment of Pediatric Respiratory Medicine, Immunology and Critical Care Medicine and Cystic Fibrosis Center, Charité - Universitätsmedizin Berlin, Berlin, GermanyBerlin Institute of Health (BIH), Berlin, GermanyInstitute of Medical Microbiology and Virology, Technische Universität Dresden, Dresden, GermanyDepartment of Infectious Diseases, Medical Microbiology and Hygiene, University of Heidelberg, Heidelberg, GermanyTranslational Lung Research Center Heidelberg (TLRC), German Center for Lung Research (DZL), University of Heidelberg, Heidelberg, GermanyProgressive impairment in lung function caused by chronic polymicrobial airway infection remains the major cause of death in patients with cystic fibrosis (CF). Cross-sectional studies suggest an association between lung function decline and specific lung microbiome ecotypes. However, longitudinal studies on the stability of the airway microbiome are missing for adolescents with CF constituting the age group showing the highest rate of decline in lung function. In this study, we analyzed longitudinal lung function data and sputum samples collected over a period of 3 to 5 years from 12 adolescents with CF. The sputum microbiome was analyzed using 16S rRNA gene sequencing. Our results indicate that the individual course of the lung microbiome is associated with longitudinal lung function. In our cohort, patients with a dynamic, diverse microbiome showed a slower decline of lung function measured by FEV1% predicted, whereas a more stable and less diverse lung microbiome was related to worse outcomes. Specifically, a higher abundance of the phyla Bacteroidetes and Firmicutes was linked to a better clinical outcome, while Proteobacteria were correlated with a decline in FEV1% predicted. Our study indicates that the stability and diversity of the lung microbiome and the abundance of Bacteroidetes and Firmicutes are associated with the lung function decline and are one of the contributing factors to the disease severity.https://www.frontiersin.org/articles/10.3389/fcimb.2021.763121/fulllung microbiomecystic fibrosislongitudinal studyvolatility analysisPseudomonas aeruginosaadolescent |
| spellingShingle | Marisa I. Metzger Marisa I. Metzger Simon Y. Graeber Simon Y. Graeber Simon Y. Graeber Mirjam Stahl Mirjam Stahl Mirjam Stahl Olaf Sommerburg Olaf Sommerburg Olaf Sommerburg Marcus A. Mall Marcus A. Mall Marcus A. Mall Alexander H. Dalpke Sébastien Boutin Sébastien Boutin A Volatile and Dynamic Longitudinal Microbiome Is Associated With Less Reduction in Lung Function in Adolescents With Cystic Fibrosis Frontiers in Cellular and Infection Microbiology lung microbiome cystic fibrosis longitudinal study volatility analysis Pseudomonas aeruginosa adolescent |
| title | A Volatile and Dynamic Longitudinal Microbiome Is Associated With Less Reduction in Lung Function in Adolescents With Cystic Fibrosis |
| title_full | A Volatile and Dynamic Longitudinal Microbiome Is Associated With Less Reduction in Lung Function in Adolescents With Cystic Fibrosis |
| title_fullStr | A Volatile and Dynamic Longitudinal Microbiome Is Associated With Less Reduction in Lung Function in Adolescents With Cystic Fibrosis |
| title_full_unstemmed | A Volatile and Dynamic Longitudinal Microbiome Is Associated With Less Reduction in Lung Function in Adolescents With Cystic Fibrosis |
| title_short | A Volatile and Dynamic Longitudinal Microbiome Is Associated With Less Reduction in Lung Function in Adolescents With Cystic Fibrosis |
| title_sort | volatile and dynamic longitudinal microbiome is associated with less reduction in lung function in adolescents with cystic fibrosis |
| topic | lung microbiome cystic fibrosis longitudinal study volatility analysis Pseudomonas aeruginosa adolescent |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2021.763121/full |
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