Significance of Neuropilin-1 (CD 304) Expression in Paediatric B- Lineage Acute Lymphoblastic Leukemia (ALL)
Background: To determine the prognostic significance of NRP-1 (CD304) expression in paediatric B-lineage Acute Lymphoblastic Leukaemia (ALL) patients. Methods: In this comparative study newly diagnosed cases (aged 1-15 years) of B-ALL were selected. Age and sex matched, 21 healthy controls we...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Rawalpindi Medical University
2019-05-01
|
| Series: | Journal of Rawalpindi Medical College |
| Subjects: | |
| Online Access: | https://www.journalrmc.com/index.php/JRMC/article/view/1075 |
| _version_ | 1818964627441057792 |
|---|---|
| author | Moizza Sahar Fauzia Shafi Khan |
| author_facet | Moizza Sahar Fauzia Shafi Khan |
| author_sort | Moizza Sahar |
| collection | DOAJ |
| description | Background: To determine the prognostic
significance of NRP-1 (CD304) expression in
paediatric B-lineage Acute Lymphoblastic
Leukaemia (ALL) patients.
Methods: In this comparative study newly
diagnosed cases (aged 1-15 years) of B-ALL were
selected. Age and sex matched, 21 healthy controls
were also included in the study to assess the NRP-1
expression on peripheral blood lymphocytes. A
minimum 1ml of blood and bone marrow aspirate
samples were taken in EDTA vacutainer and
immunophenotyping was done on gated blast cells
using an extensive panel of antibodies including
myeloid markers (CD13, CD33 and cytoplasmic anti-
MPO) and lymphoid markers (CD34, CD2, CD3,
CD5, CD7, CD4, CD8, HLA DR, CD10, CD19, CD22;
cytoplasmic CD3, CD22, CD79a and nuclear anti-
TdT). The sample was considered NRP-1 positive if
20% or more of the gated blast cells expressed it .
Man-Witney U test and Kruskal Wallis test were
used for non-parametric data. The p value <0.05 was
considered significant.
Results: Out of 66 B-ALL patients, 53% were males.
There were 20 (30%) NRP-1 positive and 46 (70%)
NRP-1 negative patients. The prognosis of NRP-1
positive group was poor as compared to NRP-1
negative group with high blast percentage (80%) (p=
0.042), low morphological remission rate (21%)
(p=0.004) and low survival rate (29%) (p=0.009). The
mean survival days in dead patients was also less
(22.75 days).
Conclusion: NRP-1 over expression is associated
with disease progression and severity in paediatric
B-ALL patients. |
| first_indexed | 2024-12-20T13:04:07Z |
| format | Article |
| id | doaj.art-d8db1de5648d4580ab68597231918f1e |
| institution | Directory Open Access Journal |
| issn | 1683-3562 1683-3570 |
| language | English |
| last_indexed | 2024-12-20T13:04:07Z |
| publishDate | 2019-05-01 |
| publisher | Rawalpindi Medical University |
| record_format | Article |
| series | Journal of Rawalpindi Medical College |
| spelling | doaj.art-d8db1de5648d4580ab68597231918f1e2022-12-21T19:39:50ZengRawalpindi Medical UniversityJournal of Rawalpindi Medical College1683-35621683-35702019-05-01231Significance of Neuropilin-1 (CD 304) Expression in Paediatric B- Lineage Acute Lymphoblastic Leukemia (ALL)Moizza Sahar0Fauzia Shafi Khan1Department of Haematology, University of Health SciencesChildren Hospital and Institute of Child Health,Ferozpur Road, LahoreBackground: To determine the prognostic significance of NRP-1 (CD304) expression in paediatric B-lineage Acute Lymphoblastic Leukaemia (ALL) patients. Methods: In this comparative study newly diagnosed cases (aged 1-15 years) of B-ALL were selected. Age and sex matched, 21 healthy controls were also included in the study to assess the NRP-1 expression on peripheral blood lymphocytes. A minimum 1ml of blood and bone marrow aspirate samples were taken in EDTA vacutainer and immunophenotyping was done on gated blast cells using an extensive panel of antibodies including myeloid markers (CD13, CD33 and cytoplasmic anti- MPO) and lymphoid markers (CD34, CD2, CD3, CD5, CD7, CD4, CD8, HLA DR, CD10, CD19, CD22; cytoplasmic CD3, CD22, CD79a and nuclear anti- TdT). The sample was considered NRP-1 positive if 20% or more of the gated blast cells expressed it . Man-Witney U test and Kruskal Wallis test were used for non-parametric data. The p value <0.05 was considered significant. Results: Out of 66 B-ALL patients, 53% were males. There were 20 (30%) NRP-1 positive and 46 (70%) NRP-1 negative patients. The prognosis of NRP-1 positive group was poor as compared to NRP-1 negative group with high blast percentage (80%) (p= 0.042), low morphological remission rate (21%) (p=0.004) and low survival rate (29%) (p=0.009). The mean survival days in dead patients was also less (22.75 days). Conclusion: NRP-1 over expression is associated with disease progression and severity in paediatric B-ALL patients.https://www.journalrmc.com/index.php/JRMC/article/view/1075NRP-1/CD304Pediatric B-lineage ALL PatientsMinimal Residual Disease(MRD),Flow Cytometry |
| spellingShingle | Moizza Sahar Fauzia Shafi Khan Significance of Neuropilin-1 (CD 304) Expression in Paediatric B- Lineage Acute Lymphoblastic Leukemia (ALL) Journal of Rawalpindi Medical College NRP-1/CD304 Pediatric B-lineage ALL Patients Minimal Residual Disease(MRD), Flow Cytometry |
| title | Significance of Neuropilin-1 (CD 304) Expression in Paediatric B- Lineage Acute Lymphoblastic Leukemia (ALL) |
| title_full | Significance of Neuropilin-1 (CD 304) Expression in Paediatric B- Lineage Acute Lymphoblastic Leukemia (ALL) |
| title_fullStr | Significance of Neuropilin-1 (CD 304) Expression in Paediatric B- Lineage Acute Lymphoblastic Leukemia (ALL) |
| title_full_unstemmed | Significance of Neuropilin-1 (CD 304) Expression in Paediatric B- Lineage Acute Lymphoblastic Leukemia (ALL) |
| title_short | Significance of Neuropilin-1 (CD 304) Expression in Paediatric B- Lineage Acute Lymphoblastic Leukemia (ALL) |
| title_sort | significance of neuropilin 1 cd 304 expression in paediatric b lineage acute lymphoblastic leukemia all |
| topic | NRP-1/CD304 Pediatric B-lineage ALL Patients Minimal Residual Disease(MRD), Flow Cytometry |
| url | https://www.journalrmc.com/index.php/JRMC/article/view/1075 |
| work_keys_str_mv | AT moizzasahar significanceofneuropilin1cd304expressioninpaediatricblineageacutelymphoblasticleukemiaall AT fauziashafikhan significanceofneuropilin1cd304expressioninpaediatricblineageacutelymphoblasticleukemiaall |