Bioinformatic identification of cassava miRNAs differentially expressed in response to infection by Xanthomonas axonopodis pv. manihotis
<p>Abstract</p> <p>Background</p> <p>microRNAs (miRNAs) are short RNA molecules that control gene expression by silencing complementary mRNA. They play a crucial role in stress response in plants, including biotic stress. Some miRNAs are known to respond to bacterial in...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-02-01
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| Series: | BMC Plant Biology |
| Online Access: | http://www.biomedcentral.com/1471-2229/12/29 |
| _version_ | 1818213107612254208 |
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| author | Pérez-Quintero Álvaro L Quintero Andrés Urrego Oscar Vanegas Pablo López Camilo |
| author_facet | Pérez-Quintero Álvaro L Quintero Andrés Urrego Oscar Vanegas Pablo López Camilo |
| author_sort | Pérez-Quintero Álvaro L |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>microRNAs (miRNAs) are short RNA molecules that control gene expression by silencing complementary mRNA. They play a crucial role in stress response in plants, including biotic stress. Some miRNAs are known to respond to bacterial infection in <it>Arabidopsis thaliana </it>but it is currently unknown whether these responses are conserved in other plants and whether novel species-specific miRNAs could have a role in defense.</p> <p>Results</p> <p>This work addresses the role of miRNAs in the <it>Manihot esculenta </it>(cassava)-<it>Xanthomonas axonopodis </it>pv. manihotis <it>(Xam) </it>interaction. Next-generation sequencing was used for analyzing small RNA libraries from cassava tissue infected and non-infected with <it>Xam</it>. A full repertoire of cassava miRNAs was characterized, which included 56 conserved families and 12 novel cassava-specific families. Endogenous targets were predicted in the cassava genome for many miRNA families. Some miRNA families' expression was increased in response to bacterial infection, including miRNAs known to mediate defense by targeting auxin-responding factors as well as some cassava-specific miRNAs. Some bacteria-repressed miRNAs included families involved in copper regulation as well as families targeting disease resistance genes. Putative transcription factor binding sites (TFBS) were identified in the <it>MIRNA </it>genes promoter region and compared to promoter regions in miRNA target genes and protein coding genes, revealing differences between <it>MIRNA </it>gene transcriptional regulation and other genes.</p> <p>Conclusions</p> <p>Taken together these results suggest that miRNAs in cassava play a role in defense against <it>Xam</it>, and that the mechanism is similar to what's known in <it>Arabidopsis </it>and involves some of the same families.</p> |
| first_indexed | 2024-12-12T05:59:02Z |
| format | Article |
| id | doaj.art-d8dd64ecd3044ea1a83d00beabe01f14 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-12T05:59:02Z |
| publishDate | 2012-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-d8dd64ecd3044ea1a83d00beabe01f142022-12-22T00:35:29ZengBMCBMC Plant Biology1471-22292012-02-011212910.1186/1471-2229-12-29Bioinformatic identification of cassava miRNAs differentially expressed in response to infection by Xanthomonas axonopodis pv. manihotisPérez-Quintero Álvaro LQuintero AndrésUrrego OscarVanegas PabloLópez Camilo<p>Abstract</p> <p>Background</p> <p>microRNAs (miRNAs) are short RNA molecules that control gene expression by silencing complementary mRNA. They play a crucial role in stress response in plants, including biotic stress. Some miRNAs are known to respond to bacterial infection in <it>Arabidopsis thaliana </it>but it is currently unknown whether these responses are conserved in other plants and whether novel species-specific miRNAs could have a role in defense.</p> <p>Results</p> <p>This work addresses the role of miRNAs in the <it>Manihot esculenta </it>(cassava)-<it>Xanthomonas axonopodis </it>pv. manihotis <it>(Xam) </it>interaction. Next-generation sequencing was used for analyzing small RNA libraries from cassava tissue infected and non-infected with <it>Xam</it>. A full repertoire of cassava miRNAs was characterized, which included 56 conserved families and 12 novel cassava-specific families. Endogenous targets were predicted in the cassava genome for many miRNA families. Some miRNA families' expression was increased in response to bacterial infection, including miRNAs known to mediate defense by targeting auxin-responding factors as well as some cassava-specific miRNAs. Some bacteria-repressed miRNAs included families involved in copper regulation as well as families targeting disease resistance genes. Putative transcription factor binding sites (TFBS) were identified in the <it>MIRNA </it>genes promoter region and compared to promoter regions in miRNA target genes and protein coding genes, revealing differences between <it>MIRNA </it>gene transcriptional regulation and other genes.</p> <p>Conclusions</p> <p>Taken together these results suggest that miRNAs in cassava play a role in defense against <it>Xam</it>, and that the mechanism is similar to what's known in <it>Arabidopsis </it>and involves some of the same families.</p>http://www.biomedcentral.com/1471-2229/12/29 |
| spellingShingle | Pérez-Quintero Álvaro L Quintero Andrés Urrego Oscar Vanegas Pablo López Camilo Bioinformatic identification of cassava miRNAs differentially expressed in response to infection by Xanthomonas axonopodis pv. manihotis BMC Plant Biology |
| title | Bioinformatic identification of cassava miRNAs differentially expressed in response to infection by Xanthomonas axonopodis pv. manihotis |
| title_full | Bioinformatic identification of cassava miRNAs differentially expressed in response to infection by Xanthomonas axonopodis pv. manihotis |
| title_fullStr | Bioinformatic identification of cassava miRNAs differentially expressed in response to infection by Xanthomonas axonopodis pv. manihotis |
| title_full_unstemmed | Bioinformatic identification of cassava miRNAs differentially expressed in response to infection by Xanthomonas axonopodis pv. manihotis |
| title_short | Bioinformatic identification of cassava miRNAs differentially expressed in response to infection by Xanthomonas axonopodis pv. manihotis |
| title_sort | bioinformatic identification of cassava mirnas differentially expressed in response to infection by xanthomonas axonopodis pv manihotis |
| url | http://www.biomedcentral.com/1471-2229/12/29 |
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