Molecular Identification of Mycoplasma Hominis in Vaginal Sample of Women Referred to the Infertility Center of Fatemieh Hospital in Hamadan
Background and Aim: Mycoplasma hominis is one of the smallest bacteria isolated from a natural source and is sometime as pathologic flora in plants, animals, and humans. Some of these bacteria are normal flora of the respiratory and genital systems. The aim of the study was molecular identification...
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Kurdistan University of Medical Sciences
2022-08-01
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| Series: | مجله علمی دانشگاه علوم پزشکی کردستان |
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| Online Access: | http://sjku.muk.ac.ir/article-1-6880-en.html |
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| author | Hadi Hossainpour Mohammad Yousef Alikhani Rasoul Yousefi Mashouf Manouchehr Karami Soghra Rabiee |
| author_facet | Hadi Hossainpour Mohammad Yousef Alikhani Rasoul Yousefi Mashouf Manouchehr Karami Soghra Rabiee |
| author_sort | Hadi Hossainpour |
| collection | DOAJ |
| description | Background and Aim: Mycoplasma hominis is one of the smallest bacteria isolated from a natural source and is sometime as pathologic flora in plants, animals, and humans. Some of these bacteria are normal flora of the respiratory and genital systems. The aim of the study was molecular identification of M. hominis in a vaginal sample of women referred to the infertility center of Fatemieh Hospital in Hamadan.
Materials and Methods: In this Cross-sectional study, vaginal swab samples were collected from symptomatic females and M. hominis was identified by PCR and Real-Time PCR. DNA was extracted using an extraction kit. M. hominis strains were identified using 16 S rRNA gene. Finally, Statistical analysis was performed using SPSS 21 software.
Results: A total of 234 vaginal samples were studied in women with a mean age of 39.8years. The results showed that M. hominis was identified by PCR (13%) and Real-time PCR (15%) methods, respectively. There was a significant relationship between PCR and Real-Time PCR methods (P≤0.05).
Conclusions: Real-time PCR assay was successfully used for rapid and accurate diagnosis of M.hominis. This method has significant potential for rapid, accurate, and is highly sensitive molecular detection of M. hominis compared to the PCR technic. |
| first_indexed | 2024-04-11T00:13:40Z |
| format | Article |
| id | doaj.art-d8dd8d205f5b491d94d5034b35503e6f |
| institution | Directory Open Access Journal |
| issn | 1560-652X 2345-4040 |
| language | fas |
| last_indexed | 2024-04-11T00:13:40Z |
| publishDate | 2022-08-01 |
| publisher | Kurdistan University of Medical Sciences |
| record_format | Article |
| series | مجله علمی دانشگاه علوم پزشکی کردستان |
| spelling | doaj.art-d8dd8d205f5b491d94d5034b35503e6f2023-01-09T06:11:35ZfasKurdistan University of Medical Sciencesمجله علمی دانشگاه علوم پزشکی کردستان1560-652X2345-40402022-08-012733644Molecular Identification of Mycoplasma Hominis in Vaginal Sample of Women Referred to the Infertility Center of Fatemieh Hospital in HamadanHadi Hossainpour0Mohammad Yousef Alikhani1Rasoul Yousefi Mashouf2Manouchehr Karami3Soghra Rabiee4 MSc student of Microbiology, School of Medicine, Hamedan University of Medical Sciences, Hamedan, Iran Professor of bacteriology, School of Medicine, Hamedan University of Medical Sciences, Hamedan, Iran Professor of Microbiology, School of Medicine, Hamedan University of Medical Sciences, Hamedan, Iran, Professor of Epidemiology, Hamadan University of Medical Sciences, Hamadan, Iran Professor of Obstetrics and gynecology, Hamadan University of Medical Sciences, Hamadan, Iran Background and Aim: Mycoplasma hominis is one of the smallest bacteria isolated from a natural source and is sometime as pathologic flora in plants, animals, and humans. Some of these bacteria are normal flora of the respiratory and genital systems. The aim of the study was molecular identification of M. hominis in a vaginal sample of women referred to the infertility center of Fatemieh Hospital in Hamadan. Materials and Methods: In this Cross-sectional study, vaginal swab samples were collected from symptomatic females and M. hominis was identified by PCR and Real-Time PCR. DNA was extracted using an extraction kit. M. hominis strains were identified using 16 S rRNA gene. Finally, Statistical analysis was performed using SPSS 21 software. Results: A total of 234 vaginal samples were studied in women with a mean age of 39.8years. The results showed that M. hominis was identified by PCR (13%) and Real-time PCR (15%) methods, respectively. There was a significant relationship between PCR and Real-Time PCR methods (P≤0.05). Conclusions: Real-time PCR assay was successfully used for rapid and accurate diagnosis of M.hominis. This method has significant potential for rapid, accurate, and is highly sensitive molecular detection of M. hominis compared to the PCR technic.http://sjku.muk.ac.ir/article-1-6880-en.htmlmycoplasma hominisinfertile womenpcrreal-time pcr |
| spellingShingle | Hadi Hossainpour Mohammad Yousef Alikhani Rasoul Yousefi Mashouf Manouchehr Karami Soghra Rabiee Molecular Identification of Mycoplasma Hominis in Vaginal Sample of Women Referred to the Infertility Center of Fatemieh Hospital in Hamadan مجله علمی دانشگاه علوم پزشکی کردستان mycoplasma hominis infertile women pcr real-time pcr |
| title | Molecular Identification of Mycoplasma Hominis in Vaginal Sample of Women Referred to the Infertility Center of Fatemieh Hospital in Hamadan |
| title_full | Molecular Identification of Mycoplasma Hominis in Vaginal Sample of Women Referred to the Infertility Center of Fatemieh Hospital in Hamadan |
| title_fullStr | Molecular Identification of Mycoplasma Hominis in Vaginal Sample of Women Referred to the Infertility Center of Fatemieh Hospital in Hamadan |
| title_full_unstemmed | Molecular Identification of Mycoplasma Hominis in Vaginal Sample of Women Referred to the Infertility Center of Fatemieh Hospital in Hamadan |
| title_short | Molecular Identification of Mycoplasma Hominis in Vaginal Sample of Women Referred to the Infertility Center of Fatemieh Hospital in Hamadan |
| title_sort | molecular identification of mycoplasma hominis in vaginal sample of women referred to the infertility center of fatemieh hospital in hamadan |
| topic | mycoplasma hominis infertile women pcr real-time pcr |
| url | http://sjku.muk.ac.ir/article-1-6880-en.html |
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